Cloning, microbial expression and structure-activity relationship of polyphenol oxidases from Camellia sinensis

Journal of Biotechnology

Volumn 145, Issue 1, 2010, Pages 66-72

  Wu, Yi Liang ^a   Pan, Li Ping ^a   Yu, Si Li ^a   Li, Hai Hang ^a  

^a SOUTH CHINA NORMAL UNIVERSITY   (China)

Author keywords

Camellia sinensis; Pichia pastoris; Polyphenol oxidase; Prokaryotic expression; Structure activity relationship; Yeast expression

Indexed keywords

CAMELLIA SINENSIS; PICHIA PASTORIS; POLYPHENOL OXIDASE; PROKARYOTIC EXPRESSION; STRUCTURE ACTIVITY RELATIONSHIPS; YEAST EXPRESSION;

AMINATION; AMINES; AMINO ACIDS; BLOCK CODES; CHLOROPHYLL; CLONING; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; NUCLEIC ACIDS; ORGANIC ACIDS; YEAST;

ENZYMES;

CATECHOL OXIDASE;

ARTICLE; BINDING SITE; BIOTECHNOLOGICAL PRODUCTION; CARBOXY TERMINAL SEQUENCE; CHLOROPLAST; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE EXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; TEA;

AMINO ACID SEQUENCE; CAMELLIA SINENSIS; CATECHOL OXIDASE; CLONING, MOLECULAR; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTATION; PICHIA; PLANT PROTEINS; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP;

CAMELLIA SINENSIS; ESCHERICHIA COLI; PICHIA PASTORIS; PROKARYOTA;

EID: 71549130403     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.10.008     Document Type: Article

References (26)

1. Bleve G., Lezzi C., Mita G., Rampino P., Perrotta C., Villanova L., and Grieco F. Molecular cloning and heterologous expression of a laccase gene from Pleurotus eryngii in free and immobilized Saccharomyces cerevisiae cells. Appl. Microbiol. Biotechnol. 79 (2008) 731-741
2. Bradford M.A. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
3. Chevalier T., de Rigal D., Mbéguié-A-Mbéguié D., Gauillard F., Richard-Forget F., and Fils-Lycaon B.R. Molecular cloning and characterization of apricot fruit polyphenol oxidase. Plant Physiol. 119 (1999) 1261-1270
4. Coseteng M.Y., and Lee C.Y. Changes in apple polyphenol oxidase and polyphenol concentrations in relation to degree of browning. J. Food Sci. 52 (1987) 985-989
5. Cout S.R., and Herrera J.L.T. Industrial and biotechnological applications of laccases: a review. Biotechnol. Adv. 24 (2006) 500-513
6. Dubé E., Shareck F., Hurtubise Y., Daneault C., and Beauregard M. Homologous cloning, expression, and characterisation of a laccase from Streptomyces coelicolor and enzymatic decolourisation of an indigo dye. Appl. Microbiol. Biotechnol. 79 (2008) 597-603
7. Faraco V., Ercole C., Festa G., Giardina P., Piscitelli A., and Sannia G. Heterologous expression of heterodimeric laccase from Pleurotus ostreatus in Kluyveromyces lactis. Appl. Microbiol. Biotechnol. 77 (2008) 1329-1335
8. Fernandes S.C., Moccelini S.K., Scheeren C.W., Migowski P., Dupont J., Heller M., Micke G.A., and Vieira I.C. Biosensor for chlorogenic acid based on an ionic liquid containing iridium nanoparticles and polyphenol oxidase. Talanta 79 (2009) 222-228
9. Guo M., Lu F., Du L., Pu J., and Bai D. Optimization of the expression of a laccase gene from Trametes versicolor in Pichia methanolica. Appl. Microbiol. Biotechnol. 71 (2006) 848-852
10. Hong Y.Z., Zhou H.M., Tu X.M., Li J.F., and Xiao Y.Z. Cloning of a laccase gene from a novel basidiomycete Trametes sp. 420 and its heterologous expression in Pichia pastoris. Curr. Microbiol. 54 (2007) 260-265
11. Hunt M.D., Eannetta N.T., Yu H., Newmann S.M., and Steffens J.C. cDNA cloning and expression of potato polyphenol oxidase. Plant Mol. Biol. 21 (1993) 59-68
12. Khan A.A., and Husain Q. Decolorization and removal of textile and non-textile dyes from polluted wastewater and dyeing effluent by using potato (Solanum tuberosum) soluble and immobilized polyphenol oxidase. Bioresour. Technol. 98 (2007) 1012-1019
13. Kiiskinen L.L., and Saloheimo M. Molecular cloning and expression in Saccharomyces cerevisiae of a laccase gene from the ascomycete Melanocarpus albomyces. Appl. Environ. Microbiol. 70 (2004) 137-144
14. Kunamneni A., Camarero S., García-Burgos C., Plou F.J., Ballesteros A., and Alcalde M. Engineering and applications of fungal laccases for organic synthesis. Microb. Cell Fact. 7 (2008) 32
15. Liu W., Chao Y., Liu S., Bao H., and Qian S. Molecular cloning and characterization of a laccase gene from the basidiomycete Fome lignosus and expression in Pichia pastoris. Appl. Microbiol. Biotechnol. 63 (2003) 174-181
16. Marston F.A., Lowe P.A., Doel M.T., Schoemaker J.M., White S., and Angal S. Purification of calf prochymosin (Prorennin) synthesized in Escherichia coli. Bio/Technology 2 (1984) 800-804
17. Mayer A.M. Polyphenol oxidases in plants and fungi: going places? A review. Phytochemistry 67 (2006) 2318-2331
18. Mikolasch A., and Schauer F. Fungal laccases as tools for the synthesis of new hybrid molecules and biomaterials. Appl. Microbiol. Biotechnol. 82 (2009) 605-624
19. Murray M.G., and Thompson W.F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8 (1980) 4321-4326
20. Riva S. Laccases: blue enzymes for green chemistry. Trends Biotechnol. 24 (2006) 219-226
21. Salony G.N., Baranwal R., Chhabra M., Mishra S., Chaudhuri T.K., and Bisaria V.S. Laccase of Cyathus bulleri: structural, catalytic characterization and expression in Escherichia coli. Biochim. Biophys. Acta 1784 (2008) 259-268
22. Shahar T., Hennig N., Gutfinger T., Hareven D.E., and Lifschitz E. The tomato 66.3-kD polyphenoloxidase gene: Molecular identification and developmental expression. Plant Cell 4 (1992) 135-147
23. Sullivan M.L., Hatfield R.D., Thoma S.L., and Samac D.A. Cloning and characterization of red clover polyphenol oxidase cDNAs and expression of activity protein in Escherichia coli and transgenic alfalfa. Plant Physiol. 136 (2004) 3234-3244
24. Tanaka T., Mine C., Inoue K., Matsuda M., and Kouno I. Synthesis of theaflavin from epicatechin and epigallocatechin by plant homogenates and role of epicatechin quinone in the syntesis and degradation of theaflavin. Agric. Food Chem. 50 (2002) 2142-2148
25. Tu Y.Y., Xu X.Q., Xia H.L., and Watanabe N. Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodology. Biotechnol. Lett. 27 (2005) 269-274
26. Zhao Y.C., Fu R., Mo C.H., and Li M.H. Degradation dynamics of DDT in contaminated soil using laccase. Environ. Chem. 27 (2008) 476-480