Partial purification and characterization of latent polyphenol oxidase in iceberg lettuce (Lactuca sativa L.)

Journal of Agricultural and Food Chemistry

Volumn 44, Issue 4, 1996, Pages 984-988

  Chazarra, Soledad ^a   Cabanes, Juana ^a   Escribano, Josefa ^a   Garcia Carmona, Francisco ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

Activation; Latency; Lettuce; Plant polyphenol oxidase; Triton X 114

Indexed keywords

LACTUCA; LACTUCA SATIVA;

EID: 0001566768     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf9501352     Document Type: Article

References (32)

1. Angleton, E. L.; Flurkey, W. H. Activation and alteration of plant and fungal polyphenoloxidase isoenzymes in sodium dodecyl sulfate electrophoresis. Phytochemistry 1984, 23, 2723-2725.
2. Arnon, D. I. Cooper enzymes in isolated chloroplast polyphenoloxidase in Beta vulgaris. Plant Physiol. 1949, 24, 1-15.
3. Bordier, C. Phase separation of integral membrane proteins in Triton X-114 solution. J. Biol. Chem. 1981, 256, 1604-1607.
4. Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
5. Flurkey, W. H. Polyphenoloxidase in higher plants. Inmunological detection and analysis of in vitro translation products. Plant Physiol. 1986, 81, 614-618.
6. Fujita, S.; Tono, T. Enzymatic browning of inmature fruit of Satsuma mandarin and its chlorogenic acid oxidase activity. Nippon Shokuhin Kogyo Gakkaishi 1981, 28, 600-605.
7. Fujita, S.; Tono, T.; Kawahara, H. Purification and properties of polyphenoloxidase in head lettuce (Lactuca sativa L). J. Sci. Food Agric. 1991, 55, 643-651.
8. Garcia-Carmona, F.; Valero, E.; Cabanes, J. Effects of L-proline on mushroom tyrosinase. Phytochemistry 1988, 27, 1961-1964.
9. Golbeck, J. H.; Cammarata, K. V. Spinach thylakoid polyphenoloxidase. Isolation, activation and properties of native chloroplast enzyme. Plant Physiol. 1981, 67, 977-984.
10. Horowitz, W., Ed.; Official and Tentative Methods of Analysis, 11th ed.; Association of Official Agricultural Chemists: Washington, DC, 1970; p 154.
11. Hutcheson, S. W.; Buchanan, B. B. Polyphenol oxidation by Vicia faba chloroplast membranes. Studies on the latent membrane-bound polyphenol oxidase and on the mechanism of photochemical polyphenol oxidation. Plant Physiol. 1980, 66, 1150-1154.
12. Jimenez-Atienzar, M.; Pedren̄o, M. A.; Garcia-Carmona, F. Activation of polyphenol oxidase by polyamines. Biochem. Int. 1991, 25, 861-868.
13. Ke, D.; Salveit, M. E., Jr. Plant hormone interaction and phenolic metabolism in the regulation of russet spotting in iceberg lettuce. Plant Physiol. 1988, 88, 1136-1140.
14. Kenten, R. H. Latent phenolase in extracts of broad bean (Vicia faba L.) 1. Activation by acid and alkali. Biochem. J. 1957, 67, 300-307.
15. Kenten, R. H. Latent phenolase in extracts of broad bean (Vicia faba L.) 1. Activation by anionic wetting agents. Biochem. J. 1958, 68, 244-251.
16. King, R. S.; Flurkey, W. H. Effects of limited proteolysis on broad bean polyphenoloxidase. J. Sci. Food Agric. 1987, 41, 231-240.
17. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
18. Link, G. E.; Gardner, M, W. Market disease of vegetables. Phytopathology 1919, 9, 497-520.
19. Matheis, G.; Whitaker, J. R. Modifications of proteins by polyphenoloxidase and peroxidase and their products. J. Food Biochem. 1984, 8, 137-162.
20. Mayer, A. M. Polyphenol oxidases in plants - recent progress. Phytochemistry 1987, 26, 11-20.
21. Mayer, A. M.; Harel, E. Polyphenol oxidases in plants - review. Phytochemistry 1979, 18, 193-218.
22. Moore, B. M.; Flurkey, W. H. Sodium dodecyl sulfate activation of a plant polyphenoloxidase. Effect of sodium dodecyl sulfate on enzymatic and physical characteristics of purified broad bean polyphenoloxidase. J. Biol Chem. 1990, 265, 4982-4990.
23. Paulson, A. T.; Vondersteoep J.; Porritt, S. W. Enzymatic browning of peaches: effect of gibberellic acid and ethephon on phenolic compounds and polyphenol oxidase activity. J. Food Sci. 1980, 45, 341-351.
24. Ródriguez-López, J. N.; Escribano, J.; García-Cánovas, F. A continuous spectophotometric method for the determination of monophenolase activity of tyrosinase using 3-methyl-2-benzothiazoline hydrazone. Anal. Biochem. 1994, 216, 205-212.
25. Sánchez-Ferrer, A.; Villalba, J.; Garcia-Carmona, F. Triton X-114 as a tool for purifying spinach polyphenol oxidase. Phytochemistry 1989, 91, 1321-1325.
26. Sánchez-Ferrer, A.; Bru, R.; Garcia-Carmona, F. Partial purification of a thylakoid-bound enzyme using temperature-induced phase partitioning. Anal. Biochem. 1990, 184, 279-282.
27. Sánchez-Ferrer, A.; Laveda, F.; Garcia-Carmona, F. Substrate-dependent activation of latent potato leaf polyphenol oxidase by anionic surfactans. J. Agric. Food Chem. 1993, 41, 1583-1586.
28. Schaller, D. R.; Von Elbe, J. H. Polyphenolics in Montmorency cherries. J. Food Sci. 1970, 35, 762-765.
29. Sharples, G. C.; Fazio, S.; Bessey, P. M. Oxidase activity and rib discoloration in Great Lakes lettuce in relation to seasonal temperature. Proc. Am. Soc. Hortic. Sci. 1963, 391-396.
30. Soderhall, I.; Soderhall, K. Purification of prophenol oxidase from Daucus carota cell cultures. Phytochemistry 1989, 28, 1805-1808.
31. Swain, T.; Mapson, L. W.; Robb, D. A. Activation of Vicia faba (L) tyrosinase as effected by denaturing agents. Phytochemistry 1966, 5, 469-482.
32. Waite, J. H. Calculating extinction coefficients for enzymatically produced o-quinones. Anal. Biochem. 1976, 75, 211-218.