Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens

PLoS ONE

Volumn 7, Issue 8, 2012, Pages

  Strezoska, Žaklina ^a   Licon, Abel ^a   Haimes, Josh ^a   Spayd, Katie Jansen ^a   Patel, Kruti M ^a   Sullivan, Kevin ^a   Jastrzebski, Katarzyna ^b   Simpson, Kaylene J ^b,c   Leake, Devin ^a   van Brabant Smith, Anja ^a   Vermeulen, Annaleen ^a  

^a Thermo Fisher Scientific   (United States)

^b PETER MACCALLUM CANCER CENTRE   (Australia)

^c UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

SHORT HAIRPIN RNA;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; DNA BARCODING; DNA ISOLATION; EMBRYO; GENE AMPLIFICATION; GENE DOSAGE; GENE LIBRARY; HUMAN; HUMAN CELL; MICROARRAY ANALYSIS; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; RNA INTERFERENCE; RNA SEQUENCE; SIGNAL TRANSDUCTION;

GENE LIBRARY; HEK293 CELLS; HELA CELLS; HUMANS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING;

EID: 84864755778     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0042341     Document Type: Article

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