High-throughput RNA interference screening using pooled shRNA libraries and next generation sequencing

Genome Biology

Volumn 12, Issue 10, 2011, Pages

  Sims, David ^a   Mendes Pereira, Ana M ^a   Frankum, Jessica ^a   Burgess, Darren ^a   Cerone, Maria Antonietta ^a   Lombardelli, Cristina ^a   Mitsopoulos, Costas ^a   Hakas, Jarle ^a   Murugaesu, Nirupa ^a   Isacke, Clare M ^a   Fenwick, Kerry ^a   Assiotis, Ioannis ^a   Kozarewa, Iwanka ^a   Zvelebil, Marketa ^a   Ashworth, Alan ^a   Lord, Christopher J ^a  

^a INSTITUTE OF CANCER RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

ANALYTIC METHOD; ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; DNA BARCODING; DNA DETERMINATION; DNA PACKAGING; GENE TECHNOLOGY; HIGH THROUGHPUT SCREENING; INTERMETHOD COMPARISON; MATHEMATICAL COMPUTING; MOLECULAR LIBRARY; NONHUMAN; PROCESS OPTIMIZATION; REPRODUCIBILITY; RNA INTERFERENCE; SENSITIVITY ANALYSIS; SEQUENCE ANALYSIS; VIRAL GENE DELIVERY SYSTEM; ALGORITHM; BIOLOGY; CELL STRAIN HEK293; COMPUTER INTERFACE; GENE LIBRARY; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HUMAN; LENTIVIRINAE; METHODOLOGY; NUCLEOTIDE SEQUENCE; PLASMID; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT;

ALGORITHMS; BASE SEQUENCE; COMPUTATIONAL BIOLOGY; GENE LIBRARY; GENETIC VECTORS; HEK293 CELLS; HUMANS; LENTIVIRUS; PLASMIDS; REPRODUCIBILITY OF RESULTS; RNA INTERFERENCE; RNA, SMALL INTERFERING; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, RNA; TRANSFECTION; USER-COMPUTER INTERFACE;

EID: 80054744229     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/gb-2011-12-10-r104     Document Type: Article

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