Live imaging of induced and controlled DNA double-strand break formation reveals extremely low repair by homologous recombination in human cells

Oncogene

Volumn 31, Issue 30, 2012, Pages 3495-3504

  Shahar, O D ^a   Ram, E V S R ^a   Shimshoni, E ^a   Hareli, S ^a   Meshorer, E ^a   Goldberg, M ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

DNA double strand breaks; DNA repair; genomic stability; homologous recombination; ISceI

Indexed keywords

DNA;

ACCURACY; ARTICLE; CONTROLLED STUDY; DNA END JOINING REPAIR; DNA REPAIR; DOUBLE STRANDED DNA BREAK; GENOMIC INSTABILITY; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; HUMAN GENOME; IMAGE ANALYSIS; IMAGING SYSTEM; PRIORITY JOURNAL;

CELL CYCLE; CELL LINE; CELL TRACKING; DEOXYRIBONUCLEASE I; DNA BREAKS, DOUBLE-STRANDED; GENOME, HUMAN; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY; RECOMBINATIONAL DNA REPAIR; TIME-LAPSE IMAGING;

MAMMALIA;

EID: 84864342422     PISSN: 09509232     EISSN: 14765594     Source Type: Journal    
DOI: 10.1038/onc.2011.516     Document Type: Article

References (29)

1. Barlow JH, Lisby M, Rothstein R. (2008). Differential regulation of the cellular response to DNA double-strand breaks in G1. Mol Cell 30: 73-85. (Pubitemid 351470149)
2. Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM. (2009). Limiting the persistence of a chromosome break diminishes its mutagenic potential. PLoS Genet 5: e1000683.
3. Brandeis M, Hunt T. (1996). The proteolysis of mitotic cyclins in mammalian cells persists from the end of mitosis until the onset of S phase. EMBO J 15: 5280-5289. (Pubitemid 26336211)
4. Chang DC, Xu N, Luo KQ. (2003). Degradation of cyclin B is required for the onset of anaphase in Mammalian cells. J Biol Chem 278: 37865-37873. (Pubitemid 37175315)
5. Covo S, de Villartay JP, Jeggo PA, Livneh Z. (2009). Translesion DNA synthesis-assisted non-homologous end-joining of complex doublestrand breaks prevents loss of DNA sequences in mammalian cells. Nucleic Acids Res 37: 6737-6745.
6. Fung TK, Poon RY. (2005). A roller coaster ride with the mitotic cyclins. Semin Cell Dev Biol 16: 335-342.
7. Hartlerode AJ, Scully R. (2009). Mechanisms of double-strand break repair in somatic mammalian cells. Biochem J 423: 157-168.
8. Honma M, Sakuraba M, Koizumi T, Takashima Y, Sakamoto H, Hayashi M. (2007). Non-homologous end-joining for repairing ISceI-induced DNA double strand breaks in human cells. DNA Repair (Amst) 6: 781-788. (Pubitemid 46670087)
9. Jeggo PA. (1998). DNA breakage and repair. Adv Genet 38: 185-218.
10. Lukusa T, Fryns JP. (2008). Human chromosome fragility. Biochim Biophys Acta 1779: 3-16.
11. Mao Z, Bozzella M, Seluanov A, Gorbunova V. (2008). DNA repair by nonhomologous end joining and homologous recombination during cell cycle in human cells. Cell Cycle 7: 2902-2906.
12. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G et al. (2006). A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 124: 1283-1298.
13. Nakanishi K, Yang YG, Pierce AJ, Taniguchi T, Digweed M, D'Andrea AD et al. (2005). Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair. Proc Natl Acad Sci USA 102: 1110-1115. (Pubitemid 40170734)
14. Pierce AJ, Johnson RD, Thompson LH, Jasin M. (1999). XRCC3 promotes homology-directed repair of DNA damage in mammalian cells. Genes Dev 13: 2633-2638. (Pubitemid 29508550)
15. Rothkamm K, Kruger I, Thompson LH, Lobrich M. (2003). Pathways of DNA double-strand break repair during the mammalian cell cycle. Mol Cell Biol 23: 5706-5715. (Pubitemid 36951334)
16. Saleh-Gohari N, Helleday T. (2004). Conservative homologous recombination preferentially repairs DNA double-strand breaks in the S phase of the cell cycle in human cells. Nucleic Acids Res 32: 3683-3688. (Pubitemid 39157716)
17. Salpeter SJ, Klochendler A, Weinberg-Corem N, Porat S, Granot Z, Shapiro AM et al. (2011). Glucose regulates cyclin D2 expression in quiescent and replicating pancreatic B-cells through glycolysis and calcium channels. Endocrinology 152: 2589-2598.
18. Sartori AA, Lukas C, Coates J, Mistrik M, Fu S, Bartek J et al. (2007). Human CtIP promotes DNA end resection. Nature 450: 509-514. (Pubitemid 350190253)
19. Schaaf MJ, Cidlowski JA. (2003). Molecular determinants of glucocorticoid receptor mobility in living cells: the importance of ligand affinity. Mol Cell Biol 23: 1922-1934. (Pubitemid 36278806)
20. Schwartz M, Zlotorynski E, Goldberg M, Ozeri E, Rahat A, le Sage C et al. (2005). Homologous recombination and nonhomologous endjoining repair pathways regulate fragile site stability. Genes Dev 19: 2715-2726. (Pubitemid 41627938)
21. Shibata A, Conrad S, Birraux J, Geuting V, Barton O, Ismail A et al. (2011). Factors determining DNA double-strand break repair pathway choice in G2 phase. EMBO J 30: 1079-1092.
22. Shrivastav M, De Haro LP, Nickoloff JA. (2008). Regulation of DNA double-strand break repair pathway choice. Cell Res 18: 134-147.
23. Soutoglou E, Dorn JF, Sengupta K, Jasin M, Nussenzweig A, Ried T et al. (2007). Positional stability of single double-strand breaks in mammalian cells. Nat Cell Biol 9: 675-682. (Pubitemid 47214730)
24. Takashima Y, Sakuraba M, Koizumi T, Sakamoto H, Hayashi M, Honma M. (2009). Dependence of DNA double strand break repair pathways on cell cycle phase in human lymphoblastoid cells. Environ Mol Mutagen 50: 815-822.
25. Takata M, Sasaki MS, Sonoda E, Morrison C, Hashimoto M, Utsumi H et al. (1998). Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells. EMBO J 17: 5497-5508. (Pubitemid 28427063)
26. Villalobos MJ, Betti CJ, Vaughan AT. (2006). Detection of DNA double-strand breaks and chromosome translocations using ligation-mediated PCR and inverse PCR. Methods Mol Biol 314: 109-121.
27. Wersto RP, Chrest FJ, Leary JF, Morris C, Stetler-Stevenson MA, Gabrielson E. (2001). Doublet discrimination in DNA cell-cycle analysis. Cytometry 46: 296-306. (Pubitemid 33055231)
28. Yanez RJ, Porter AC. (1998). Therapeutic gene targeting. Gene Ther 5: 149-159. (Pubitemid 28077741)
29. Zur A, Brandeis M. (2001). Securin degradation is mediated by fzy and fzr, and is required for complete chromatid separation but not for cytokinesis. EMBO J 20: 792-801. (Pubitemid 32167643)