Structural biology: Structure of a V3-containing HIV-1 gp120 core

Science

Volumn 310, Issue 5750, 2005, Pages 1025-1028

  Huang, Chih Chin ^a   Tang, Min ^a   Zhang, Mei Yun ^b   Majeed, Shahzad ^a   Montabana, Elizabeth ^a   Stanfield, Robyn L ^d   Dimitrov, Dimiter S ^c   Korber, Bette ^e   Sodroski, Joseph ^f   Wilson, Ian A ^d   Wyatt, Richard ^a   Kwong, Peter D ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b SAIC FREDERICK INC   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

^e LOS ALAMOS NATIONAL LABORATORY   (United States)

^f HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CELL MEMBRANES; IMMUNOLOGY; VIRUSES;

CELL-SURFACE; IMMUNODOMINANCE; NEUTRALIZATION;

ANTIBODIES;

CD4 ANTIGEN; ENVELOPE PROTEIN; GLYCOPROTEIN GP 120; NEUTRALIZING ANTIBODY; VIRUS GLYCOPROTEIN; CHEMOKINE RECEPTOR CCR5; CHEMOKINE RECEPTOR CXCR4; EPITOPE; HIV ENVELOPE PROTEIN GP120 (305 321); HIV ENVELOPE PROTEIN GP120 (305-321); HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY; PEPTIDE FRAGMENT;

CELLS AND CELL COMPONENTS;

AMINO ACID SEQUENCE; ANIMAL CELL; ANTIBODY RESPONSE; ANTIGEN BINDING; ANTIGENIC VARIATION; ARTICLE; CELL SURFACE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; HUMAN IMMUNODEFICIENCY VIRUS 1; IMMUNE RESPONSE; IMMUNOGLOBULIN VARIABLE REGION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; RECEPTOR BINDING; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; VIRUS ADSORPTION; VIRUS CORE; VIRUS NEUTRALIZATION; X RAY CRYSTALLOGRAPHY; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; CRYSTALLIZATION; HUMAN; HYDROGEN BOND; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS 1;

AMINO ACID SEQUENCE; ANTIGENS, CD4; BINDING SITES; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; HIV ANTIBODIES; HIV ENVELOPE PROTEIN GP120; HIV-1; HUMANS; HYDROGEN BONDING; IMMUNODOMINANT EPITOPES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CCR5; RECEPTORS, CXCR4;

EID: 27744597054     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1118398     Document Type: Article

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40. We thank J. Robinson for CD4i antibodies 17b, 48d, and 412d; J. Hoxie and G. Lin for discussions regarding the effects of V3 deletion on HIV entry; E. Berger, G. Nabel, and L. Shapiro for comments on the manuscript; and Syrrx for assistance with robotic screening. Supported by the Intramural Research Program of the NIH, Vaccine Research Center (R.W. and P.D.K.), and National Cancer Institute (D.S.D.) and by the International AIDS Vaccine Initiative (J.S. and I.A.W.), NIH grants AI24755, AI31783, AI39429, AI40895 (J.S.) and GM46192 (I.A.W.), and a grant from the Bill and Melinda Gates Foundation Grand Challenges in Global Health Initiative (D.S.D., R.W., and P.D.K.). Use of SER-CAT at the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science, under contract W-31-109-Eng-38. Coordinates and structure factors have been deposited with the Protein Data Bank under accession number 2B4C and are also available from the authors.