Online size-exclusion high-performance liquid chromatography light scattering and differential refractometry methods to determine degree of polymer conjugation to proteins and protein-protein or protein-ligand association states

Analytical Biochemistry

Volumn 299, Issue 2, 2001, Pages 136-146

  Kendrick, Brent S ^a   Kerwin, Bruce A ^a   Chang, Byeong S ^a   Philo, John S ^b  

^a AMGEN INC   (United States)

^b ALLIANCE PROTEIN LABORATORIES   (United States)

Author keywords

Differential refractometry; Glycosylation; Light scattering; Online SEC HPLC; Pegylation; Protein ligand interactions

Indexed keywords

GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGANDS; LIGHT SCATTERING; MOLECULES; REFRACTIVE INDEX; SIZE EXCLUSION CHROMATOGRAPHY;

BRAIN-DERIVED NEUROTROPHIC FACTORS; EXTINCTION COEFFICIENTS; PEGYLATION; PROTEIN-LIGAND ASSOCIATION; PROTEIN-LIGAND INTERACTIONS; PROTEIN-POLYMER CONJUGATES; REFRACTOMETRY; SEC-HPLC;

PROTEINS;

BRAIN DERIVED NEUROTROPHIC FACTOR; ERYTHROPOIETIN; MACROGOL; POLYPEPTIDE; PROTEIN; RIBONUCLEASE A;

ANIMAL CELL; ARTICLE; BIOTECHNOLOGY; CONTROLLED STUDY; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGAND BINDING; LIGHT SCATTERING; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN GLYCOSYLATION; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; REFRACTOMETRY; ULTRAVIOLET RADIATION;

EID: 0035894420     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5411     Document Type: Article

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