Bifunctionality of the thiamin diphosphate cofactor: Assignment of tautomeric/ionization states of the 4′-aminopyrimidine ring when various intermediates occupy the active sites during the catalysis of yeast pyruvate decarboxylase

Journal of the American Chemical Society

Volumn 134, Issue 8, 2012, Pages 3873-3885

  Balakrishnan, Anand ^b   Gao, Yuhong ^b   Moorjani, Prerna ^b   Nemeria, Natalia S ^b   Tittmann, Kai ^a   Jordan, Frank ^b  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID BASE; ACTIVE CENTER; ACTIVE SITE; AMINO ACID METABOLISM; AMINOPYRIMIDINES; BIOSYNTHETIC PATHWAY; C-C BOND FORMING; COFACTORS; ELECTROSTATIC MODELS; ENZYMATIC REACTION; IONIZATION STATE; NON-OXIDATIVE; PYRUVATE DECARBOXYLASE; PYRUVATES; RATE-LIMITING STEPS; STEADY STATE; SUBSTRATE ACTIVATION; TAUTOMERIZATIONS; THIAMIN DIPHOSPHATE; TIME-RESOLVED; TIME-RESOLVED CIRCULAR DICHROISM;

AMINO ACIDS; CARBOXYLATION; CATALYSIS; CHEMICAL BONDS; ENZYMES; IONIZATION; SUGARS; YEAST;

REACTION INTERMEDIATES;

4' AMINOPYRIMIDINE; CARBON; COCARBOXYLASE; PHOSPHINE DERIVATIVE; PROPIONYLPHOSPHINATE; PYRIMIDINE; PYRUVATE DECARBOXYLASE; PYRUVIC ACID; UNCLASSIFIED DRUG;

AMINO ACID METABOLISM; ARTICLE; CARBOHYDRATE METABOLISM; CATALYSIS; CATALYST; CHEMICAL BOND; CIRCULAR DICHROISM; CONTROLLED STUDY; COVALENT BOND; DECARBOXYLATION; ELECTROPHILICITY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; IONIZATION; NONHUMAN; NONOXIDATIVE DECARBOXYLATION; NUCLEAR MAGNETIC RESONANCE; PROTEIN FAMILY; STATIC ELECTRICITY; SUBSTITUTION REACTION; TAUTOMER; TAUTOMERIZATION; YEAST;

BIOCATALYSIS; CATALYTIC DOMAIN; MODELS, MOLECULAR; MOLECULAR STRUCTURE; PYRIMIDINES; PYRUVATE DECARBOXYLASE; SACCHAROMYCES CEREVISIAE; THIAMINE PYROPHOSPHATE;

EID: 84863277043     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja211139c     Document Type: Article

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