Glutamate 636 of the Escherichia coli pyruvate dehydrogenase-E1 participates in active center communication and behaves as an engineered acetolactate synthase with unusual stereoselectivity

Journal of Biological Chemistry

Volumn 280, Issue 22, 2005, Pages 21473-21482

  Nemeria, Natalia ^a   Tittmann, Kai ^b   Joseph, Ebenezer ^a   Zhou, Leon ^a   Vazquez Coll, Michelle B ^a,c   Arjunan, Palaniappa ^d,e   Hübner, Gerhard ^b   Furey, William ^d,e   Jordan, Frank ^a  

^a RUTGERS UNIVERSITY   (United States)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^c UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^d UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^e VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; COMPLEXATION; ENZYME KINETICS; ESCHERICHIA COLI; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; REACTION KINETICS;

COVALENT COMPLEX; FLUORESCENCE BINDING; TAUTOMERS; VARIANTS;

AMINO ACIDS;

1',4' IMINOPYRIMIDINE; ACETOLACTATE SYNTHASE; ACETOLACTIC ACID; BACTERIAL ENZYME; COCARBOXYLASE; GLUTAMIC ACID; LACTIC ACID DERIVATIVE; PHOSPHONOLACTYLTHIAMINE DIPHOSPHATE; PYRIMIDINE DERIVATIVE; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVATE DEHYDROGENASE E1; THIAMINE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CIRCULAR DICHROISM; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBUNIT; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN FOLDING; STEREOCHEMISTRY;

ACETOLACTATE SYNTHASE; ASPARTIC ACID; BINDING SITES; CATALYSIS; CIRCULAR DICHROISM; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; GLUTAMIC ACID; KINETICS; LACTATES; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXYGEN; PEPTIDES; PLASMIDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PYRIMIDINE NUCLEOSIDES; PYRUVATE DEHYDROGENASE (LIPOAMIDE); SPECTROMETRY, FLUORESCENCE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; STEREOISOMERISM; TEMPERATURE; THIAMINE PYROPHOSPHATE; TRYPSIN; ULTRAVIOLET RAYS; VARIATION (GENETICS);

ESCHERICHIA COLI;

EID: 20444368424     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M502691200     Document Type: Article

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