Communication between thiamin cofactors in the Escherichia coli pyruvate dehydrogenase complex E1 component active centers: Evidence for a "direct pathway" between the 4′-aminopyrimidine N1′ atoms

Journal of Biological Chemistry

Volumn 285, Issue 15, 2010, Pages 11197-11209

  Nemeria, Natalia S ^a   Arjunan, Palaniappa ^b,c   Chandrasekhar, Krishnamoorthy ^c   Mossad, Madouna ^a   Tittmann, Kai ^d   Furey, William ^b,c   Jordan, Frank ^a  

^a RUTGERS UNIVERSITY   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDIC RESIDUES; ACTIVE CENTER; ACTIVE SITE; AMINOPYRIMIDINES; CIRCULAR DICHROISM; COFACTOR BINDING; COFACTORS; DIPHOSPHATES; DIRECT CONTACT; FLUORESCENCE QUENCHING; HYDROGEN/DEUTERIUM EXCHANGE; PYRUVATE DEHYDROGENASE COMPLEX; SIGNAL TRANSDUCTION PATHWAYS; STEADY-STATE KINETICS; STRUCTURAL BASIS; SUBSTITUTION AFFECTS;

ATOMIC SPECTROSCOPY; DICHROISM; ESCHERICHIA COLI; SIGNAL TRANSDUCTION; SPECTROSCOPIC ANALYSIS; STRUCTURAL ANALYSIS;

CRYSTAL ATOMIC STRUCTURE;

ARGININE; COCARBOXYLASE; DEUTERIUM; GLUTAMIC ACID; HYDROGEN; PHOSPHONIC ACID DERIVATIVE; PYRIMIDINE; PYRUVATE DEHYDROGENASE COMPLEX; THIAMINE;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; COVALENT BOND; CRYSTAL STRUCTURE; DECARBOXYLATION; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; FLUORESCENCE SPECTROSCOPY; KINETICS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; SIGNAL TRANSDUCTION; STEADY STATE; STRUCTURE ANALYSIS; TITRIMETRY;

4-AMINOPYRIDINE; CATALYSIS; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; PROTEIN BINDING; PYRUVATE DEHYDROGENASE (LIPOAMIDE); THIAMINE; THIAMINE PYROPHOSPHATE; VITAMIN B COMPLEX;

ESCHERICHIA COLI;

EID: 77951218305     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.069179     Document Type: Article

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