Improved thermal performance of Thermomyces lanuginosus GH11 xylanase by engineering of an N-terminal disulfide bridge

Bioresource Technology

Volumn 112, Issue , 2012, Pages 275-279

  Wang, Yawei ^a,b   Fu, Zheng ^a,b   Huang, Huoqing ^b   Zhang, Huashan ^c   Yao, Bin ^b   Xiong, Hairong ^a   Turunen, Ossi ^d  

^a SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c HUBEI UNIVERSITY OF TECHNOLOGY   (China)

^d AALTO UNIVERSITY   (Finland)

Author keywords

Disulfide bridge; GH11 xylanase; Protein engineering; Thermomyces lanuginosus DSM10635; Thermostability

Indexed keywords

DISULFIDE BRIDGE; PROTEIN ENGINEERING; THERMOMYCES LANUGINOSUS DSM10635; THERMOSTABILITY; XYLANASES;

ALKALINITY; CIRCULAR DICHROISM SPECTROSCOPY; ENZYMES; GENETIC ENGINEERING;

COVALENT BONDS;

DISULFIDE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

BIOENGINEERING; ENZYME; FUNGUS; INDUSTRIAL PRODUCTION; MELTING; PH; PROTEIN; SUBSTRATE; SULFIDE (ORGANIC); TEMPERATURE PROFILE; THERMODYNAMIC PROPERTY; THERMOPHILY;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME STABILITY; HALF LIFE TIME; MELTING POINT; NONHUMAN; PH; PRIORITY JOURNAL; TEMPERATURE MEASUREMENT; THERMAL STIMULATION; THERMODYNAMICS; THERMOMYCES LANUGINOSUS; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

ALKALIES; ASCOMYCOTA; CIRCULAR DICHROISM; DISULFIDES; ENDO-1,4-BETA XYLANASES; ENZYME ACTIVATION; ENZYME STABILITY; HALF-LIFE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MUTATION; PROTEIN ENGINEERING; TEMPERATURE; TIME FACTORS; TRANSITION TEMPERATURE;

THERMOMYCES LANUGINOSUS;

EID: 84862812009     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2012.02.092     Document Type: Article

References (33)

1. Anbarasan S., Paloheimo M., Jänis J., Leisola M., Turunen O. Effect of pH, glycosylation and additional domains on the thermostability of family 10 xylanase of Thermopolyspora flexuosa. Appl. Environ. Microbiol. 2010, 76:356-360.
2. Bajpai P. Biological bleaching of chemical pulps. Crit. Rev. Biotechnol. 2004, 24:1-58.
3. Beg Q.K., Kapoor M., Mahajan L., Hoondal G.S. Microbial xylanases and their industrial applications: a review. Appl. Microbiol. Biotechnol. 2001, 56:326-338.
4. Chapla, D., Pandit, P., Shah, A., 2011. Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics. Bioresour. Technol. (In Press).
5. Collins T., Gerday C., Feller G. Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol. Rev. 2005, 29:3-23.
6. Fenel F., Leisola M., Jänis J., Turunen O. A de novo designed N-terminal disulfide bridge stabilizes the Trichoderma reesei endo-1,4-beta-xylanase II. J. Biotechnol. 2004, 108:137-143.
7. Gruber K., Klintschar G., Hayn M., Schlacher A., Steiner W., Kratky C. Thermophilic xylanase from Thermomyces lanuginosus: high-resolution X-ray structure and modeling studies. Biochemistry 1998, 37:13475-13485.
8. Guex N., Peitsch M.C. SWISS-MODEL and the Swiss-Pdb-Viewer: an environment for comparative protein modelling. Electrophoresis 1997, 18:2714-2723.
9. Haki G.D., Rakshit S.K. Developments in industrially important thermostable enzymes: a review. Bioresour. Technol. 2003, 89:17-34.
10. Hakulinen N., Turunen O., Jänis J., Leisola M., Rouvinen J. Three-dimensional structures of thermophilic β-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa comparison of twelve xylanases in relation to their thermal stability. Eur. J. Biochem. 2003, 270:1399-1412.
11. Heckman K.L., Pease L.R. Gene splicing and mutagenesis by PCR-driven overlap extension. Nat. Protoc. 2007, 2:924-932.
12. Huang Y.-C., Yu-Fen Chen Y.-F., Chen C.-Y., Chen W.-L., Ciou Y.-P., Liu W.-H., Yang C.-H. Production of ferulic acid from lignocellulolytic agricultural biomass by Thermobifida fusca thermostable esterase produced in Yarrowia lipolytica transformant. Bioresour. Technol. 2011, 102:8117-8122.
13. Jänis J., Turunen O., Leisola M., Derrick P., Rouvinen J., Vainiotalo P. Characterization of mutant xylanases using fourier transform ion cyclotron resonance mass spectrometry: stabilizing contributions of disulfide bridges and N-terminal extensions. Biochemistry 2004, 43:9556-9566.
14. Li X.T., Jiang Z.Q., Li L.T., Yang S.Q., Feng W.Y., Fan J.Y., Kusakabe I. Characterization of a cellulase-free, neutral xylanase from Thermomyces lanuginosus CBS 288.54 and its biobleaching effect on wheat straw pulp. Bioresour. Technol. 2005, 96:1370-1379.
15. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193:265-275.
16. Nishimoto M., Kitaoka M., Fushinobu S., Hayashi K. The role of conserved arginine residue in loop 4 of glycoside hydrolase family 10 xylanases. Biosci. Biotechnol. Biochem. 2005, 69:904-910.
17. Paës G., O'Donohue M.J. Engineering increased thermostability in the thermostable GH-11 xylanase from Thermobacillus xylanilyticus. J. Biotechnol. 2006, 125:338-350.
18. Purmonen M., Valjakka J., Valjakka K., Laitinen T., Rouvinen J. Molecular dynamics studies on the thermostability of family 11 xylanases. Protein. Eng. Des. Sel. 2007, 20:551-559.
19. Schlacher A., Holzmann K., Hayn M., Steiner W., Schwab H. Cloning and characterization of the gene for the thermostable xylanase XynA from Thermomyces lanuginosus. J. Biotechnol. 1996, 49:211-218.
20. Singh S., Madlala A.M., Prior B.A. Thermomyces lanuginosus: properties of strains and their hemicellulases. FEMS Microbiol. Rev. 2003, 27:3-16.
21. Teng C., Yan Q., Jiang Z., Fan G., Shi B. Production of xylooligosaccharides from the steam explosion liquor of corncobs coupled with enzymatic hydrolysis using a thermostable xylanase. Bioresour. Technol. 2010, 101:7679-7682.
22. Tian J., Wang P., Gao S., Chu X., Wu N., Fan Y. Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation. FEBS J. 2010, 277:4901-4908.
23. Tolan, J., Popovici, C., Foody, P.J., 2009. Alkaline extraction stages comprising xylanase. Patent, US 7541,175 B1.
24. Turner P., Mamo G., Karlsson E.N. Potential and utilization of thermophiles and thermostable enzymes in biorefining. Microb. Cell Fact. 2007, 6:9.
25. Turunen O., Etuaho K., Fenal F., Vehmaanpera J., Wu X., Rouvinen J., Leisola M. A combination of weakly stabilizing mutations with a disulfide bridge in the α-helix region of Trichoderma reesei endo-1,4-β-xylanase II increases the thermal stability through synergism. J. Biotechnol. 2001, 88:37-46.
26. Törrönen A., Rouvinen J. Structural and functional properties of low molecular weight endo-1,4-beta-xylanases. J. Biotechnol. 1997, 57:137-149.
27. Wakarchuk W.W., Sung W.L., Campbell R.L., Cunningham A., Watson D.C., Yaguchi M. Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds. Protein Eng. Des. Sel. 1994, 7:1379-1386.
28. Xiong H., Fenel F., Leisola M., Turunen O. Engineering the thermostability of Trichoderma reesei endo-1,4-β-xylanase II by combination of disulfide bridges. Extremophiles 2004, 8:393-400.
29. Xiong H., Nyyssölä A., Jänis J., Pastinen O., von Weymarn N., Leisola M., Turunen O. Characterization of the xylanase produced by submerged cultivation of Thermomyces lanuginosus DSM10635. Enzyme Microb. Technol. 2004, 35:93-99.
30. Xiong H., Weymarn N., Turunen O., Leisola M., Pastinen O. Xylanase production by Trichoderma reesei Rut C-30 grown on l-arabinose-rich plant hydrolysates. Bioresour. Technol. 2005, 96:753-759.
31. Yang H.M., Yao B., Meng K., Wang Y.R., Bai Y.G., Wu N.F. Introduction of a disulfide bridge enhances the thermostability of a Streptomyces olivaceoviridis xylanase mutant. J. Ind. Microbiol. Biotechnol. 2007, 34:213-218.
32. You C., Huang Q., Xue H., Xu Y., Lu H. Potential hydrophobic interaction between two cysteines in interior hydrophobic region improves thermostability of a family 11 xylanase from Neocallimastix patriciarum. Biotechnol. Bioeng. 2010, 105:861-870.
33. Zhang J., Tuomainen P., Siika-aho M., Viikari L. Comparison of the synergistic action of two thermostable xylanases from GH families 10 and 11 with thermostable cellulases in lignocellulose hydrolysis. Bioresour. Technol. 2011, 102:9090-9095.