Potential hydrophobic interaction between two cysteines in interior hydrophobic region improves thermostability of a family 11 xylanase from Neocallimastix patriciarum

Biotechnology and Bioengineering

Volumn 105, Issue 5, 2010, Pages 861-870

  You, Chun ^a   Huang, Qiang ^a   Xue, Huping ^a   Xu, Yang ^a   Lu, Hong ^a,b  

^a FUDAN UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

Cysteine; Hydrophobic interaction; Thermostability; Xylanase

Indexed keywords

CIRCULAR DICHROISM; DIRECTED EVOLUTION; DISULFIDE BOND FORMATION; DISULFIDE BONDS; FAMILY 11; HOMOLOGY MODELING; HYDROPHOBIC INTERACTIONS; HYDROPHOBIC REGIONS; MELTING TEMPERATURES; SIDE-CHAIN; SITE DIRECTED MUTAGENESIS; SPECIFIC ACTIVITY; WILD TYPES; XYLANASES;

DICHROISM; MUTAGENESIS;

HYDROPHOBICITY;

5,5' DITHIOBIS(2 NITROBENZOIC ACID); CYSTEINE; DISULFIDE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; CHROMATOGRAPHY; CIRCULAR DICHROISM; DISULFIDE BOND; ENZYME ACTIVITY; MUTATION; NEOCALLIMASTIX; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; TITRIMETRY; ULTRAVIOLET RADIATION;

AMINO ACID SUBSTITUTION; CIRCULAR DICHROISM; DIRECTED MOLECULAR EVOLUTION; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; FUNGAL PROTEINS; HOT TEMPERATURE; HYDROPHOBICITY; MODELS, MOLECULAR; MUTANT PROTEINS; MUTATION, MISSENSE; NEOCALLIMASTIX; PROTEIN STRUCTURE, TERTIARY;

NEOCALLIMASTIX PATRICIARUM;

EID: 77949419505     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22623     Document Type: Article

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