Characterization of mutant xylanases using fourier transform ion cyclotron resonance mass spectrometry: Stabilizing contributions of disulfide bridges and N-terminal extensions

Biochemistry

Volumn 43, Issue 29, 2004, Pages 9556-9566

  Jänis, Janne ^a   Turunen, Ossi ^b   Leisola, Matti ^b   Derrick, Peter J ^c   Rouvinen, Juha ^a   Vainiotalo, Pirjo ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

^b AALTO UNIVERSITY   (Finland)

^c UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION; AMINO ACIDS; CONFORMATIONS; CYCLOTRON RESONANCE; DNA; ENZYMES; FOURIER TRANSFORMS; MASS SPECTROMETRY; REDUCTION; THERMODYNAMIC STABILITY;

CONFORMERS; ELECTROSPRAY IONIZATION (ESI);

BIOCHEMISTRY;

AMINO ACID; DISULFIDE; DNA; ENDO 1,4 BETA XYLANASE II; MUTANT PROTEIN; PEPTIDE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ALKYLATION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONFORMATIONAL TRANSITION; CROSS LINKING; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME INACTIVATION; ENZYME STABILITY; ENZYME STRUCTURE; ION CYCLOTRON RESONANCE MASS SPECTROMETRY; LOW TEMPERATURE; MELTING POINT; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; REDUCTION; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; TRICHODERMA REESEI;

AMINO ACID SEQUENCE; DISULFIDES; FOURIER ANALYSIS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; XYLOSIDASES;

HYPOCREA JECORINA; TRICHODERMA;

EID: 3242663327     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049597b     Document Type: Article

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