Cloning and characterization of the gene for the thermostable xylanase XynA from Thermomyces lanuginosus

Journal of Biotechnology

Volumn 49, Issue 1-3, 1996, Pages 211-218

  Schlacher, A ^a   Holzmann, K ^a,c   Hayn, M ^b   Steiner, W ^a   Schwab, H ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b UNIVERSITY OF GRAZ   (Austria)

^c UNIVERSITY OF VIENNA   (Austria)

Author keywords

Fusion protein; Thermomyces lanuginosus; Xylanase

Indexed keywords

AMINO ACIDS; COLIFORM BACTERIA; DNA SEQUENCES; ELECTROPHORESIS; ENZYMES; FUNGI; GENES; POLYPEPTIDES; PURIFICATION;

POLYACRYLAMIDE GEL ELECTROPHORESIS; THERMOMYCES LANUGINOSUS; THERMOSTABLE XYLANASE;

CLONING;

COMPLEMENTARY DNA; GLUCAN SYNTHASE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; DNA SEQUENCE; ENZYME ASSAY; ENZYME CHEMISTRY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; THERMOSTABILITY;

FUNGI; THERMOMYCES LANUGINOSUS;

EID: 0030595046     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01516-7     Document Type: Article

References (40)

1. Abelson, J.N. and Simon, M.I. (1987) Isolation of RNA using guanidinium salts. Methods Enzymol. 152, 219-227.
2. Anand, L., Krishnamurthgy, S. and Vithayathil, P.J. (1990) Purification and properties of a xylanase from the thermophilic fungus, Humicola lanuginosa (Griffon and Maublanc) Bunce. Arch. Biochem. Biophys. 276, 546-553.
3. Apel, P.C., Panaccione, D.G., Holden, F.R. and Walton, J.D. (1993) Cloning and targeted gene disruption of xyl1, a beta-1,4-xylanase gene from the maize pathogen Cochliobolus carbonum. Mol. Plant-Microbe Interact. 6, 467-473.
4. Arase, A., Yomo, C.A., Urabe, I., Hata, Y., Katsube, Y. and Okada, H. (1993) Stabilization of xylanase by random mutagenesis. FEBS Lett. 316, 123-127.
5. Ausubel, F.A., Brent, R., Kingston, RE., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (Eds.) (1995) Current Protocols in Molecular Biology, Wiley, New York.
6. Bailey, M.J., Biely, P. and Poutanen, K. (1992) Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 23, 257-270.
7. Ballance, D.J. (1986) Sequences important for gene expression in filamentous fungi. Yeast 2, 229-236.
8. Black, G.W., Hazlewood, G.P., Millward-Sadler, S.J., Laurie, J.I. and Gilbert, H.J. (1995) A modular xylanase containing a novel non-catalytic xylan-specific binding domain. Biochem. J. 307, 191-195.
9. Derewenda, U., Swenson, L., Green, R., Wei, Y.Y., Morosoli, R., Shareck, F., Kluepfel, D. and Derewenda, Z.S. (1994) Crystal-structure, at 2.6-Angstrom resolution, of the Streptomyces lividans xylanase-A, a member of the F-family of beta-1,4-D-glycanases. J. Biol. Chem. 269, 20811-20814.
10. Ferreira, L.M.A., Wood, T.M., Williamson, G., Faulds, C., Hazlewood, G.P., Black, G.W. and Gilbert, H.J. (1993) A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a noncatalytic cellulose-binding domain. Biochem. J. 294, 349-355.
11. Gomes, J., Gomes, I., Kreiner, W., Esterbauer, H., Sinner, M. and Steiner, W. (1993a) Production of high-level of cellulase-free and thermostable xylanase by a wild strain of Thermomyces lanuginosus using beechwood xylan. J. Biotechnol. 30, 283-297.
12. Gomes, J., Purkarthofer, H., Hayn, M., Kapplmüller, J., Sinner, M. and Steiner, W. (1993b) Production of a high-level of cellulase-free xylanase by the thermophilic fungus Thermomyces lanuginosus in laboratory and pilot scales using lignocellulosic materials. Appl. Microbiol. Biotechnol. 39, 700-707.
13. Haas, H., Friedlin, E., Stoffler, G. and Redl, B. (1993) Cloning and structural organization of a xylanase-encoding gene from Penicillium chrysogenum. Gene 126, 237-242.
14. Harlow, E. and Lane, D. (Eds.) (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Harris, G.W., Jenkins, J.A., Connerton, I., Cummings, N., Loleggio, L., Scott, M., Hazlewood, G.P., Laurie, J.I., Gilbert, H.J. and Pickersgill, R.W. (1994) Structure of the catalytic core of the family F-xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites. Structure 2, 1107-1116.
16. Hay, B. and Short, J.M. (1992) ExAssist helper phage and SOLR cells for lambda ZapII excissions. Stratagies 5, 16-18.
17. Hazlewood, G.P., Davidson, K., Laurie, J.I., Huskisson, N.S. and Gilbert, H.J. (1993) Gene sequence and properties of celi, a family-E endoglucanase from Clostridium thermocellum. J. Gen. Microbiol. 139, 307-316.
18. Henrissat, B. (1992) Analysis of hemicellulases sequences. Relationship to other glycanases. In: Visser, J., Kusters-van Someren, M.A., Beldman, G. and Voragen, A.G.J. (Eds.), Xylans and Xylanases, Elsevier, Amsterdam, pp. 97-110.
19. Hu, P., Chase, T. and Eveleigh, D.E. (1993) Cloning of a Microbispora bispora cellobiohydrolase gene in Streptomyces lividans. Appl. Microbiol. Biotechnol. 38, 631-637.
20. Ko, E.P., Akatsuka, H., Moriyama, H., Shinmyo, A., Hata, Y., Katsube, Y., Urabe, I. and Okada, H. (1992) Site-Directed mutagenesis at aspartate and glutamate residues of xylanase from Bacillus pumilus. Biochem. J. 288, 117-121.
21. Kolar, M., Punt, P.J., van den Hondel, C.A.M.J.J. and Schwab, H. (1988) Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene. Gene 62, 127-134.
22. Li, X.L. and Ljungdahl, L.G. (1994) Cloning, sequencing, and regulation of a xylanase gene from the fungus Aureobasidium pullulans-Y-2311-1. Appl. Environ. Microbiol. 60, 4647-4647. Correction: Appl. Environ. Microbiol. 60, 4647.
23. Mcintosh, L., Plesniak, L., Johnson, P., Withers, S. and Wakarchuk, W. (1995) The structure and enzymatic mechanism of Bacillus circulans xylanase. J. Cell. Biochem. S21B, 30-30
24. Miao, S.C., Ziser, L., Aebersold, R. and Withers, S.G. (1994) Identification of glutamic-acid-78 as the active-site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass-spectrometry. Biochemistry 33, 7027-7032
25. Millwardsadler, S.J., Poole, D.M., Henrissat, B., Hazlewood, G.P., Clarke, J.H. and Gilbert, H.J. (1994) Evidence for a general role for high-affinity noncatalytic cellulose-binding domains in microbial plant-cell wall hydrolases. Mol. Microbiol. 11, 375-382
26. Oku, T., Roy, C., Watson, D.C., Wakarchuk, W., Campbell, R., Yaguchi, M., Jurasek, L. and Paice, M.G. (1993) Amino-acid-sequence and thermostability of xylanase-A from Schizophyllum commune. FEBS Lett. 334, 296-300.
27. Purkarthofer, H., Sinner, M. and Steiner, W. (1993a) Cellulase-free xylanase from Thermomyces lanuginosus - optimization of production in submerged and solid-state culture. Enzyme Microbe Technol. 115, 677-682
28. Purkarthofer, H., Sinner, M. and Steiner, W. (1993b) Effect of shear rate and culture pH on the production of xylanase by Thermomyces lanuginosus. Biotechnol. Lett. 15, 405-410
29. Sakka, K., Kojima, Y., Kondo, T., Karita, S., Ohmiya, K. and Shimada, K. (1993) Nucleotide-sequence of the Clostridium stercorarium xyna gene encoding xylanase-A: identification of catalytic and cellulose binding domains. Biosci. Biotechnol. Biochem. 57, 273-277.
30. Tatu, U., Murthy, S.K. and Vithayathil, P.J. (1990) Role of a disulfide cross-link in the conformational stability of a thermostable xylanase. J. Protein Chem. 9, 641-646.
31. Theberge, M., Lacaze, P., Shareck, F., Morosoli, R. and Kluepfel, D. (1992) Purification and characterization of an endoglucanase from Streptomyces lividans-66 and DNA-sequence of the gene. Appl. Environ, Microbiol. 58, 815-820.
32. Törrönen, A., Mach, R.L., Messner, R., Gonzales, R., Kalkkinen, N., Harrki, A. and Kubicek, C.P. (1992) The two major xylanases from Trichoderma reesei: characterization of both enzymes and genes. Bio/Technology 10, 1461-1465.
33. Törrönen, A., Harkki, A. and Rouvinen, J. (1994) Three-dimensional structure of endo-1,4-beta-xylanase II from Trichoderma reesei: two conformational states in the active site. EMBO J. 13, 2493-2501.
34. Törrönen, A. and Rouvinen, J. (1995) Structural comparison of 2 major endo-1,4-xylanases from Trichoderma reesei. Biochemistry 34, 847-856.
35. Unkles, S.E. (1992) Gene Organization in Industrial Filamentous Fungi. In: Kinghorn, J.R. and Turner, G. (Eds.), Applied Molecular Genetics of Filamentous Fungi, Chapman and Hall, London, pp. 28-53.
36. von Heijne, G. (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14, 4683-4690.
37. Wakarchuk, W.W., Campbell, R.L., Sung W.L., Davoodi, J. and Yaguchi, M. (1994a) Mutational and crystallographic analyses of the active-site residues of the Bacillus circulans xylanase. Protein Sci. 3, 467-475.
38. Wakarchuk, W.W., Sung, W.L., Campbell, R.L., Cunningham, A., Watson, D.C. and Yaguchi, M. (1994b) Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds. Protein Eng. 7, 1379-1386.
39. Yaguchi, M., Roy, C., Ujiie, M., Watson, D.C. and Wakarchuk, W. (1992) Aminoacid sequence of the low molecular weight xylanase from Trichoderma viride. Relationship to other glycanases. In: Visser, J., Kusters-van Someren, M.A., Beldman, G. and Voragen, A.G.J. (Eds.), Xylans and Xylanases, Elsevier, Amsterdam, pp. 149-154.
40. Young, R.A. and Davis, R.W. (1983) Efficient isolation of genes by using antibody probes. Proc. Natl. Acad. Sci. USA 80, 1194-1198.