Restriction endonucleases: Natural and directed evolution

Applied Microbiology and Biotechnology

Volumn 94, Issue 3, 2012, Pages 583-599

  Gupta, Richa ^a   Capalash, Neena ^a   Sharma, Prince ^a  

^a PANJAB UNIVERSITY   (India)

Author keywords

Applications; Engineering; Evolution; Mutation; Restriction enzymes

Indexed keywords

ACTIVE SITE; CLASSIFICATION SYSTEM; CLEAVAGE MECHANISM; CLEAVAGE SPECIFICITY; DIRECTED EVOLUTION; ENHANCED PROPERTIES; ENVIRONMENTAL PRESSURES; EVOLUTION; FUNCTIONAL PROPERTIES; GENETIC RELATEDNESS; HOMOLOGOUS RECOMBINATION; ISOSCHIZOMERS; MUTATION; PROTEIN VARIANTS; RANDOM MUTATION; RESTRICTION ENDONUCLEASES; RESTRICTION ENZYMES; SEQUENCE SIMILARITY; STRUCTURAL CHANGE; STRUCTURAL CORE; SYNTHETIC MOLECULES; TYPE II;

ALKYLATION; AMINO ACIDS; APPLICATIONS; ENGINEERING; ENZYMES; MOLECULAR BIOLOGY; PROTEINS; RATIONAL FUNCTIONS;

SCAFFOLDS (BIOLOGY);

TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

AMINO ACID; BIOCHEMICAL COMPOSITION; DATABASE; ENZYME ACTIVITY; MOLECULAR ANALYSIS; MUTATION; PROTEIN; SUBSTRATE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; HUMAN; MOLECULAR EVOLUTION; MOLECULAR RECOGNITION; NONHUMAN; PHYLOGENETIC TREE; PROTEIN FUNCTION; REVIEW;

DIRECTED MOLECULAR EVOLUTION; DNA; DNA RESTRICTION ENZYMES;

EID: 84862638845     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-3961-z     Document Type: Review

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