Evolution of sequence specificity in a restriction endonuclease by a point mutation

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 30, 2008, Pages 10344-10347

  Saravanan, Matheshwaran ^a   Vasu, Kommireddy ^a   Nagaraja, Valakunja ^a,b  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

Author keywords

Genetic recombination; Metal ion coordination; Promiscuous activity; Protein engineering; R.KpnI

Indexed keywords

RESTRICTION ENDONUCLEASE;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DNA CLEAVAGE; ENZYME ACTIVITY; ENZYME KINETICS; GENE SEQUENCE; METAL BINDING; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; WILD TYPE;

BINDING SITES; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; DNA RESTRICTION ENZYMES; IONS; KINETICS; MAGNESIUM; METALS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; POINT MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC;

EID: 48749094277     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0804974105     Document Type: Article

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