On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: A case study employing EcoRV as the target

Protein Engineering

Volumn 13, Issue 4, 2000, Pages 275-281

  Lanio, Thomas ^a   Jeltsch, Albert ^a   Pingoud, Alfred ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

DNA recognition; EcoRV; Protein engineering; Rational protein design; Site directed mutagenesis; Specificity

Indexed keywords

DNA; OLIGODEOXYNUCLEOTIDE; RESTRICTION ENDONUCLEASE;

ARTICLE; BASE PAIRING; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME SPECIFICITY; GENETIC ENGINEERING; PRIORITY JOURNAL; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

EID: 0034026486     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/13.4.275     Document Type: Article

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