Effects of alpha particle radiation on gene expression in human pulmonary epithelial cells

International Journal of Hygiene and Environmental Health

Volumn 215, Issue 5, 2012, Pages 522-535

  Chauhan, Vinita ^a   Howland, Matthew ^a   Mendenhall, Amy ^b   O'Hara, Shifawn ^a   Stocki, Trevor J ^a   McNamee, James P ^a   Wilkins, Ruth C ^a  

^a HEALTH CANADA   (Canada)

^b Ingenuity Systems Inc   (United States)

Author keywords

137Cs; 214Po; 222Rn; 238Pu; 241Am; Alpha particles; AROS; CT; Cy; DCT; EDTA; Epithelial cells; FBS; FC; FDR; Gene expression; IPA; MD; Microarray; NTP; OD; PA; PBS; QPCR; Radon; TBS; TST

Indexed keywords

ALPHA RADIATION; ARTICLE; CYTOLOGY; EPITHELIUM CELL; GENE EXPRESSION PROFILING; HUMAN; LUNG; METABOLISM; RADIATION EXPOSURE; TUMOR CELL LINE;

ALPHA PARTICLES; CELL LINE, TUMOR; EPITHELIAL CELLS; GENE EXPRESSION PROFILING; HUMANS; LUNG;

EID: 84861931182     PISSN: 14384639     EISSN: 1618131X     Source Type: Journal    
DOI: 10.1016/j.ijheh.2012.04.004     Document Type: Article

References (75)

1. Al-Zoughool M., Krewski D. Health effects of radon: a review of the literature. Int. J. Radiat. Biol. 2009, 85:57-69.
2. Appleton J.D. Radon: sources, health risks, and hazard mapping. Ambio 2007, 36:85-89.
3. Batty D.P., Wood R.D. Damage recognition in nucleotide excision repair of DNA. Gene 2000, 241:193-204.
4. Bayram H., Devalia J.L., Sapsford R.J., Ohtoshi T., Miyabara Y., Sagai M., Davies R.J. The effect of diesel exhaust particles on cell function and release of inflammatory mediators from human bronchial epithelial cells in vitro. Am. J. Respir. Cell Mol. Biol. 1998, 18:441-448.
5. Beaton L.A., Burn T.A., Stocki T.J., Chauhan V., Wilkins R.C. Development and characterization of an in vitro alpha radiation exposure system. Phys. Med. Biol. 2011, 56:3645-3658.
6. Benjamini Y., Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J. R. Stat. Soc. 1995, 57:289-300.
7. Bolstad B.M., Irizarry R.A., Astrand M., Speed T.P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 2003, 19:185-193.
8. Brooks A.L., Khan M.A., Jostes R.F., Cross F.T. Metaphase chromosome aberrations as markers of radiation exposure and dose. J. Toxicol. Environ. Health 1993, 40:277-288.
9. Calvano S.E., Xiao W., Richards D.R., Felciano R.M., Baker H.V., Cho R.J., Chen R.O., Brownstein B.H., Cobb J.P., Tschoeke S.K., Miller-Graziano C., Moldawer L.L., Mindrinos M.N., Davis R.W., Tompkins R.G., Lowry S.F. A network-based analysis of systemic inflammation in humans. Nature 2005, 437:1032-1037.
10. Carmena M., Earnshaw W.C. The cellular geography of Aurora kinases. Nat. Rev. Mol. Cell Biol. 2003, 4:842-854.
11. Caswell R.S., Coyne J.J. Microdosimetry of radon and radon daughters. Radiat. Prot. Dosim. 1990, 31:395-398.
12. Crighton D., Wilkinson S., O'Prey J., Syed N., Smith P., Harrison P.R., Gasco M., Garrone O., Crook T., Ryan K.M. DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. Cell 2006, 126:121-134.
13. Crighton D., Wilkinson S., Ryan K.M. DRAM links autophagy to p53 and programmed cell death. Autophagy 2007, 3:72-74.
14. Cui X., Hwang J.T., Qiu J., Blades N.J., Churchill G.A. Improved statistical tests for differential gene expression by shrinking variance components estimates. Biostatistics 2005, 6:59-75.
15. Darby S.C., Hill D.C. European collaborative group on residential radon and lung cancer. Health effects of residential radon: a European perspective at the end of 2002. Radiat. Prot. Dosim. 2003, 104(4):321-329.
16. Darby S., Hill D., Auvinen A., Barros-Dios J.M., Baysson H., Bochicchio F., Deo H., Falk R., Forastiere F., Hakama M., Heid I., Kreienbrock L., Kreuzer M., Lagarde F., Mäkeläinen I., Muirhead C., Oberaigner W., Pershagen G., Ruano-Ravina A., Ruosteenoja E., Rosario A.S., Tirmarche M., Tomásek L., Whitley E., Wichmann H.E., Doll R. Radon in homes and risk of lung cancer: collaborative analysis of individual data from 13 European case-control studies. Br. Med. J. 2005, 330:223-231.
17. Feng C.J., Li H.J., Li J.N., Lu Y.J., Liao G.Q. Expression of Mcm7 and Cdc6 in oral squamous cell carcinoma and precancerous lesions. Anticancer Res. 2008, 28:3763-3769.
18. Feng L., Barnhart J.R., Seeger R.C., Wu L., Keshelava N., Huang S.H., Jong A. Cdc6 knockdown inhibits human neuroblastoma cell proliferation. Mol. Cell. Biochem. 2008, 311:189-197.
19. Fujii T., Hayashi S., Hogg J.C., Vincent R., Vincent S.F. Particulate matter induces cytokine expression in human bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 2001, 25:265-271.
20. Ghandhi S.A., Yaghoubian B., Amundson S.A. Global gene expression analyses of bystander and alpha particle irradiated normal human lung fibroblasts: synchronous and differential responses. BMC Med. Genomics 2008, 1:63.
21. Goodnight J.H., Harvey W.R. Least-Squares Means in the Fixed-Effects General Linear Models, SAS Technical Report R-103 1978, SAS Institute Inc, Cary, NC.
22. Henshaw D.L., Eatough J.P., Richardson R.B. Radon as a causative factor in induction of myeloid leukaemia and other cancers. Lancet 1990, 335:1008-1012.
23. Hill R., Bodzak E., Blough M.D., Lee P.W. p53 Binding to the p21 promoter is dependent on the nature of DNA damage. Cell Cycle 2008, 7:2535-2543.
24. Hu Z., Wang Y., Wang X., Liang G., Miao X., Xu Y., Tan W., Wei Q., Lin D., Shen H. DNA repair gene XPC genotypes/haplotypes and risk of lung cancer in a Chinese population. Int. J. Cancer 2005, 115:478-483.
25. IPA, Calculating and Interpreting the p-Values for Functions, Pathways, and Lists in Ingenuity Pathways Analysis, 2009. Ingenuity Systems. http://www.ingenuity.com/.
26. IPA Network Generation Algorithm, 2005. Ingenuity systems, . http://www.ingenuity.com/.
27. Iwaoka K., Tokonami S., Yonehara H., Ishikawa T., Doi M., Kobayashi Y., Yatabe Y., Takahashi H., Yamada Y. Continuous measurements of bronchial exposure induced by radon decay products during inhalation. Rev. Sci. Instrum. 2007, 78:093301-1-093301-4.
28. Jostes R.F. Genetic, cytogenetic, and carcinogenic effects of radon: a review. Mutat. Res. 1996, 340:125-139.
29. Kennedy C.A., Gray A.M., Denman A.R., Phillips P.S. The cost-effectiveness of residential radon remediation programmes: assumptions about benefits stream profiles over time. Journal of Environmental Radioactivity 2002, 59:19-28.
30. Kerr M.K., Churchill G.A. Statistical design and the analysis of gene expression microarray data. Genet. Res. 2001, 77:123-128.
31. Kerr M.K. Design considerations for efficient and effective microarray studies. Biometrics 2003, 59:822-828.
32. Kokkinakis D.M., Brickner A.G., Kirkwood J.M., Liu X., Goldwasser J.E., Kastrama A., Sander C., Bocangel D., Chada S. Mitotic arrest, apoptosis, and sensitization to chemotherapy of melanomas by methionine deprivation stress. Mol. Cancer Res. 2006, 4:575-589. ].
33. Krewski D., Lubin J.H., Zielinski J.M., Alavanja M., Catalan V.S., Field R.W., Klotz J.B., Létourneau E.G., Lynch C.F., Lyon J.I., Sandler D.P., Schoenberg J.B., Steck D.J., Stolwijk J.A., Weinberg C., Wilcox H.B. Residential radon and risk of lung cancer: a combined analysis of 7 North American case-control studies. Epidemiology 2005, 16(2):137-145.
34. Lei M. The MCM complex: its role in DNA replication and implications for cancer therapy. Curr. Cancer Drug Targets 2005, 5:365-380.
35. Levkau B., Koyama H., Raines E.W., Clurman B.E., Herren B., Orth K., Roberts J.M., Ross R. Cleavage of p21Cip1/Waf1 and p27Kip1 mediates apoptosis in endothelial cells through activation of Cdk2: role of a caspase cascade. Mol. Cell 1998, 1:553-563.
36. Li J.X., Fu C.L., Chen R., Sun J., Nie J.H., Tong J. Screening of differential expression genes in bone marrow cells of radon-exposed mice. J. Toxicol. Environ. Health A 2007, 70:964-969.
37. Liebermann D.A., Hoffman B. Gadd45 in the response of hematopoietic cells to genotoxic stress. Blood Cells Molecules and Diseases 2007, 39:329-335.
38. Little J.B., Nagasawa H., Li G.C., Chen D.J. Involvement of the nonhomologous end joining DNA repair pathway in the bystander effect for chromosomal aberrations. Radiat. Res. 2003, 159:262-267.
39. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408.
40. Löbrich M., Jeggo P.A. The impact of a negligent G2/M checkpoint on genomic instability and cancer induction. Nat. Rev. Cancer 2007, 7:861-869.
41. Longphre M., Li D., Li J., Matovinovic E., Gallup E., Samet J.M., Basbaum C.B. Lung mucin production is stimulated by the air pollutant residual oil fly ash. Toxicol. Appl. Pharmacol. 2000, 162:86-92.
42. Lutze L.H., Winegar R.A., Jostes R., Cross F.T., Cleaver J.E. Radon-induced deletions in human cells: role of nonhomologous strand rejoining. Cancer Res. 1992, 52:5126-5129.
43. Nagasawa H., Little J.B. Bystander effect for chromosomal aberrations induced in wild-type and repair deficient CHO cells by low fluences of alpha particles. Mutat. Res. 2002, 508:121-129.
44. National Research Council Health Effects of Exposure to Radon (BEIR VI) 1999, National Academy Press, Washington, DC.
45. Neuberger J.S., Gesell T.F. Childhood cancers, radon, and gamma radiation. Lancet 2002, 360:1437-1438.
46. Ohtoshi T., Takizawa H., Okazaki H., Kawasaki S., Takeuchi N., Ohta K., Ito K. Diesel exhaust particles stimulate human airway epithelial cells to produce cytokines relevant to airway inflammation in vitro. J. Allergy Clin. Immunol. 1998, 101:778-785.
47. Pohl-Rüling J., Lettner H., Hofmann W., Eckl P., Haas O.A., Obe G., Grell-Büchtmann I., van Buul P.P., Schroeder-Kurth T., Atzmüller C., Sasaki M.S., Fischer P., Kubiak R., Natarajan A.T. Chromosomal aberrations of blood lymphocytes induced in vitro by radon-222 daughter alpha-irradiation. Mutat. Res. 2000, 449:7-19.
48. Porté S., Valencia E., Yakovtseva E.A., Borràs E., Shafqat N., Debreczeny J.E., Pike A.C., Oppermann U., Farrés J., Fita I., Parés X. Three-dimensional structure and enzymatic function of proapoptotic human p53 inducible quinone oxidoreductase PIG3. J. Biol. Chem. 2009, 284:17194-17205.
49. R Development Core Team R: A Language and Environment for Statistical Computing 2005, R Foundation for Statistical Computing, Vienna, Austria. http://www.r-project.org/.
50. Samet J.M., Pathak D.R., Morgan M.V., Key C.R., Valdivia A.A., Lubin J.H. Lung cancer mortality and exposure to radon progeny in a cohort of New Mexico underground uranium miners. Health Phys. 1991, 61(6):745-752.
51. Samet J.M., Eradze G.R. Radon and lung cancer risk: taking stock at the millennium. Environ. Health Perspect. 2000, 108(Suppl. 4):635-641.
52. Schwartz J.L., Shadley J.D., Atcher R.W., Tang J., Whitlock J.L., Rotmensch J. Comparison of radon-daughter-induced effects in repair-proficient and repair-deficient CHO cell lines. Environ. Mol. Mutagen. 1990, 16:178-184.
53. Seagrave J.C., Nikula K.J. Multiple modes of responses to air pollution particulate materials in A549 alveolar type II cells. Inhal. Toxicol. 2000, 12:247-260.
54. Searle S.R., Speed F.M., Milliken G.A. The population marginal means in the linear model: an alternative to least squares means. Am. Statistician 1980, 34:216-221.
55. Shadley J.D., Whitlock J.L., Rotmensch J., Atcher R.W., Tang J., Schwartz J.L. The effects of radon daughter alpha-particle irradiation in K1 and xrs-5 CHO cell lines. Mutat. Res. 1991, 248:73-83.
56. Shannon K.B., Salmon E.D. Chromosome dynamics: new light on Aurora B kinase function. Curr. Biol. 2002, 12:R458-R460.
57. Shieh A.D., Hung Y.S. Detecting outlier samples in microarray data. Stat. Appl. Genet. Mol. Biol. 2009, 8. (Article 13. Epub 2009 Feb 11).
58. Snyder A.R., Morgan W.F. Gene expression profiling after irradiation: clues to understanding acute and persistent responses?. Cancer Metastasis Rev. 2004, 23:259-268.
59. Soto J., Sainz C., González-Lamuño D., Cos S. Low doses of alpha particle irradiation modify the expression of genes regulating apoptosis in human MCF-7 breast cancer cells. Oncol. Rep. 2006, 15:577-581.
60. Stather J.W. Dosimetric and epidemiological approaches to assessing radon doses - can the differences be reconciled?. Radiat. Prot. Dosim. 2004, 112:487-492.
61. Strauss G.H. Non-random cell killing in cryopreservation: implications for performance of the battery of leukocyte tests (BLT), I. Toxic and immunotoxic effects. Mutat. Res. 1991, 252:1-15.
62. Stringer B., Kobzik L. Environmental particulate-mediated cytokine production in lung epithelial cells (A549): role of preexisting inflammation and oxidant stress. J. Toxicol. Environ. Health A 1998, 55:31-44.
63. Taya A., Morgan A., Baker S.T., Humphreys J.A., Bisson M., Collier C.G. Changes in the rat lung after exposure to radon and its progeny: effects on incorporation of bromodeoxyuridine in epithelial cells and on the incidence of nuclear aberrations in alveolar macrophages. Radiat. Res. 1994, 139:170-177.
64. Turtoi A., Schneeweiss F.H. Effect of (211)At alpha-particle irradiation on expression of selected radiation responsive genes in human lymphocytes. Int. J. Radiat. Biol. 2009, 85:403-412.
65. Turtoi A., Brown I., Schläger M., Schneeweiss F.H. Gene expression profile of human lymphocytes exposed to (211)At alpha particles. Radiat. Res. 2010, 174:125-136.
66. Twu N.F., Yuan C.C., Yen M.S., Lai C.R., Chao K.C., Wang P.H., Wu H.H., Chen Y.J. Expression of Aurora kinase A and B in normal and malignant cervical tissue: high Aurora A kinase expression in squamous cervical cancer. Eur. J. Obstet. Gynecol. Reprod. Biol. 2008, 142:57-63.
67. Vischioni B., Oudejans J.J., Vos W., Rodriguez J.A., Giaccone G. Frequent overexpression of Aurora B kinase, a novel drug target, in non-small cell lung carcinoma patients. Mol. Cancer Ther. 2006, 5:2905-2913.
68. Wilson G.D. Radiation and the cell cycle, revisited. Cancer Metastasis Rev. 2004, 23:209-225.
69. Wood R.D., Shivji M.K. Which DNA polymerases are used for DNA-repair in eukaryotes?. Carcinogenesis 1997, 18:605-610.
70. Wright J.A., Chan A.K., Choy B.K., Hurta R.A., McClarty G.A., Tagger A.Y. Regulation and drug resistance mechanisms of mammalian ribonucleotide reductase, and the significance to DNA synthesis. Biochem. Cell Biol. 1990, 68:1364-1371.
71. Wu H., Kerr M.K., Cui X., Churchill G.A. MAANOVA: a software package for the analysis of spotted cDNA microarray experiments. The Analysis of Gene Expression Data: Methods and Software 2003, 313-431. Springer-Verlag. G. Parmigiani, E.S. Garrett, R.A. Irizarry, S. Zeger (Eds.).
72. Ying L., Sarwal M. In praise of arrays. Pediatr. Nephrol. 2009, 24:1643-1659.
73. Yu J., Zhang L. No PUMA, no death: implications for p53-dependent apoptosis. Cancer Cell 2003, 4:248-249.
74. Zhou Q., Su Y., Bai M. Effect of antisense RNA targeting Polo-like kinase 1 on cell growth in A549 lung cancer cells. J. Huazhong Univ. Sci. Technol. 2008, 28:22-26.
75. Zhu Y., Yang H., Chen Q., Lin J., Grossman H.B., Dinney C.P., Wu X., Gu J. Modulation of DNA damage/DNA repair capacity by XPC polymorphisms. DNA Repair 2008, 7:141-148.