Overexpression of membrane proteins using Pichia pastoris

Current Protocols in Protein Science

Volumn 1, Issue SUPPL.67, 2012, Pages

  Bornert, Olivier ^a   Alkhalfioui, Fatima ^a   Logez, Christel ^a   Wagner, Renaud ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

Coupled receptors; G protein; Immunodetection; Ligand binding; Membrane preparation; Membrane proteins; Pichia pastoris; Recombinant expression

Indexed keywords

ALPHA 1A ADRENERGIC RECEPTOR; BUFFER; G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; ADRA1A PROTEIN, HUMAN; ALPHA 1 ADRENERGIC RECEPTOR;

AGAR MEDIUM; ALPHA 1A ADRENERGIC RECEPTOR GENE; ARTICLE; BACTERIAL CELL; CLINICAL PROTOCOL; CLONING VECTOR; CRYSTALLIZATION; CYTOLYSIS; DNA SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; G PROTEIN COUPLED RECEPTOR GENE; IMMUNOBLOTTING; IMMUNODETECTION; LIGAND BINDING; NONHUMAN; PICHIA PASTORIS; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS DESIGN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN ISOLATION; RECEPTOR GENE; YEAST CELL; CELL MEMBRANE; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PICHIA; PRACTICE GUIDELINE; PROTEIN ENGINEERING; STANDARD;

EUKARYOTA; PICHIA PASTORIS;

CELL MEMBRANE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC VECTORS; GUIDELINES AS TOPIC; HUMANS; IMMUNOBLOTTING; MEMBRANE PROTEINS; PICHIA; PLASMIDS; PROTEIN ENGINEERING; RECEPTORS, ADRENERGIC, ALPHA-1; RECEPTORS, G-PROTEIN-COUPLED; TRANSFORMATION, GENETIC;

EID: 84861146561     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps2902s67     Document Type: Article

References (45)

1. Alkhalfioui, F., Logez, C., Bornert, O., andWagner, R. 2011. Expression systems: Pichia pastoris. In Production of Membrane Proteins-Strategies for Expression and Isolation (A.S. Robinson, ed.) pp. 75-108. Wiley-VCH, Weinheim, Germany.
2. Aller, S.G. and Unger, V.M. 2006. Projection structure of the human copper transporter CTR1 at 6-°A resolution reveals a compact trimer with a novel channel-like architecture. Proc. Natl. Acad. Sci. U.S.A. 103:3627-3632.
3. Aller, S.G., Yu, J., Ward, A., Weng, Y., Chittaboina, S., Zhuo, R., Harrell, P.M., Trinh, Y.T., Zhang, Q., Urbatsch, I.L., and Chang, G. 2009. Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding. Science 323:1718-1722.
4. André, N., Cherouati, N., Prual, C., Steffan, T., Zeder-Lutz, G., Magnin, T., Pattus, F., Michel, H., Wagner, R., and Reinhart, C. 2006. Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci. 15:1115-1126.
5. Bernier, V., Lagacé, M., Bichet, D.G., and Bouvier, M. 2004. Pharmacological chaperones: Potential treatment for conformational diseases. Trends Endocrinol. Metab. 15:222-228.
6. Binda, C., Newton-Vinson, P., Hubalek, F., Edmondson, D.E., and Mattevi, A. 2002. Structure of human monoamine oxidase B, a drug target for the treatment of neurological disorders. Nat. Struct. Biol. 9:22-26.
7. Bylund, D.B. 2007. Characterization ofAdrenoceptors. Curr. Protoc. Pharmacol. 36:1.5.1-1.5.18.
8. Cereghino, J.L. and Cregg, J.M. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:45-66.
9. Cereghino, G.P.L., Cereghino, J.L., Ilgen, C., and Cregg, J.M. 2002. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13:329-332.
10. Cohen, E., Goldshleger, R., Shainskaya, A., Tal, D.M., Ebel, C., Le Maire, M., and Karlish, S.J.D. 2005. Purification of Na, K-ATPase expressed in Pichia pastoris reveals an essential role of phospholipid-protein interactions. J. Biol. Chem. 280:16610-16618.
11. Cos, O., Ramón, R., Montesinos, J.L., and Valero, F. 2006. Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review. Microb. Cell Fact. 5:17.
12. Cregg, J.M. and Madden, K.R. 1987. Development of yeast transformation systems and construction of methanol-utilization-defective mutants of Pichia pastoris gene disruption. Biol. Res. Yeasts II:1-18.
13. Cregg, J. M., Tolstorukov, I., Kusari, A., Sunga, A. J., Madden, K., and Chappell, T. 2010. Expression of recombinant genes in the yeast Pichia pastoris. Curr. Protoc. Essential Lab. Techn. 4:13.2.1-13.2.14.
14. Eckart, M.R. and Bussineau, C.M. 1996. Quality and authenticity of heterologous proteins synthesized in yeast. Curr. Opin. Biotechnol. 7:525-530.
15. Elion, E. A., Marina, P., andYu, L. 2007. Constructing recombinant DNA molecules by PCR. Curr. Protoc. Mol. Biol. 78:3.17.1-3.17.12.
16. Fischer, G., Kosinska-Eriksson, U., Aponte-Santamaria, C., Palmgren, M., Geijer, C., Hedfalk, K., Hohmann, S., de Groot, B.L., Neutze, R., and Lindkvist-Petersson, K. 2009. Crystal structure of a yeast aquaporin at 1.15 Å reveals a novel gating mechanism. PLOS Biol. 7:e1000130.
17. Grinna, L.S. and Tschopp, J.F. 1989. Size distribution and general structural features of N-linked oligosaccharides from the methylotrophic yeast, Pichia pastoris. Yeast 5:107-115.
18. Grunewald, S., Haase, W., Molsberger, E., Michel, H., and Reilander, H. 2004. Production of the humanD2S receptor in the methylotrophic yeast P. pastoris. Receptors Channels 10:37-50.
19. Hamilton, S.R. and Gerngross, T.U. 2007. Glycosylation engineering in yeast: The advent of fully humanized yeast. Curr. Opin. Biotechnol. 18:387-392.
20. Hartner, F.S. and Glieder, A. 2006. Regulation of methanol utilisation pathway genes in yeasts. Microb. Cell Fact. 5:39.
21. +-ATPase purified from Pichia pastoris membranes by specific interactions with lipids. Biochemistry 46:12855-12867.
22. Horsefield, R., Nordén, K., Fellert, M., Backmark, A., Tornroth-Horsefield, S., Terwisscha van Scheltinga, A.C., Kvassman, J., Kjellbom, P., Johanson, U., and Neutze, R. 2008. Highresolution x-ray structure of human aquaporin 5. Proc. Natl. Acad. Sci. U.S.A. 105:13327-13332.
23. Li, P., Anumanthan, A., Gao, X.G., Ilangovan, K., Suzara, V.V., Düzgünes, N., and Renugopalakrishnan, V. 2007. Expression of recombinant proteins in Pichia pastoris. Appl. Biochem. Biotechnol. 142:105-124.
24. Lifshitz, Y., Petrovich, E., Haviv, H., Goldshleger, R., Tal, D.M., Garty, H., and Karlish, S.J.D. 2007. Purification of the human α2 isoform of Na, K-ATPase expressed in Pichia pastoris. Stabilization by lipids and FXYD1. Biochemistry 46:14937-14950.
25. + channel. Science 309:897-903.
26. Long, S.B., Tao, X., Campbell, E.B., and MacKinnon, R. 2007. Atomic structure of a voltage dependent K+channel in a lipid membranelike environment. Nature 450:376-382.
27. Lundstrom, K., Wagner, R., Reinhart, C., Desmyter, A., Cherouati, N., Magnin, T., Zeder-Lutz, G., Courtot, M., Prual, C., André, N., Hassaine, G., Michel, H., Cambillau, C., and Pattus, F. 2006. Structural genomics on membrane proteins: Comparison of more than 100 GPCRs in 3 expression systems. J. Struct. Funct. Genomics 7:77-91.
28. Martinez Molina, D., Wetterholm, A., Kohl, A., McCarthy, A.A., Niegowski, D., Ohlson, E., Hammarberg, T., Eshaghi, S., Haeggstrom, J.Z., and Nordlund, P. 2007. Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase. Nature 448:613-617.
29. Opekarova, M. and Tanner, W. 2003. Specific lipid requirements of membrane protein-a putative bottleneck in heterologous expression. Biochim. Biophys. Acta 1610:11-22.
30. + channels heterologously expressed in Pichia pastoris. J. Mol. Biol. 333:103-116.
31. Reina, C., Padoani, G., Carotti, C., Merico, A., Tripodi, G., Ferrari, P., and Popolo, L. 2007. Expression of the α3/β1 isoform of human Na, KATPase in the methylotrophic yeast Pichia pastoris. FEMS Yeast Res. 7:585-594.
32. Reinhart, C. and Krettler, C. 2006. Expression of membrane proteins in yeasts. In Structural Genomics and Rational Drug Design inMembrane Proteins (K. Lundstrom, ed.) pp. 115-152. Marcel Dekker, New York.
33. Sarramegna, V., Talmont, F., Seree de Roch, M., Milon, A., and Demange, P. 2002. Green fluorescent protein as a reporter of human μ-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris. J. Biotechnol. 99:23-39.
34. Sarramegna, V., Talmont, F., Demange, P., and Milon, A. 2003. Heterologous expression of Gprotein-coupled receptors: comparison of expression systems fron the standpoint of largescale production and purification. Cell Mol. Life Sci. 60:1529-1546.
35. Shukla, A.K., Haase, W., Reinhart, C., and Michel, H. 2007. Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells. Int. J. Biochem. Cell Biol. 39:931-942.
36. Struhl, K. 2000. Subcloning of DNA fragments. Curr. Protoc. Mol. Biol. 13:3.16.1-3.16.2.
37. + channel Kir2.2 at 3.1 Å resolution. Science 326:1668-1674.
38. Tate, C.G., Haase, J., Baker, C., Boorsma, M., Magnani, F., Vallis, Y., and Williams, D.C. 2003. Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter. Biochim. Biophys. Acta 1160:141-153.
39. Tornroth-Horsefield, S., Wang, Y., Hedfalk, K., Johanson, U., Karlsson, M., Tajkhorshid, E., Neutze, R., and Kjellbom, P. 2006. Structural mechanism of plant aquaporin gating. Nature 439:688-694.
40. Weiss, H.M., Haase, W., Michel, H., and Reilander, H. 1995. Expression of functional mouse 5-HT5A serotonin receptor in the methylotrophic yeast Pichia pastoris: Pharmacological characterization and localization. FEBS Lett. 377:451-456.
41. Weiss, H.M., Haase, W., Michel, H., and Reilander, H. 1998. Comparative biochemical and pharmacological characterization of the mouse 5HT5A 5-hydroxytryptamine receptor and the human β2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris. Biochem. J. 330:1137-1147.
42. White, C.E., Hunter, M.J., Meininger, D.P., White, L.R., and Komives, E.A. 1995. Large-scale expression, purification and characterization of small fragments of thrombomodulin: The roles of the sixth domain and of methionine 388. Protein Eng. 8:1177-1187.
43. Whittaker, M.M. and Whittaker, J.W. 2005. Construction and characterization of Pichia pastoris strains for labeling aromatic amino acids in recombinant proteins. Protein Expr. Purif. 41:266-274.
44. Wood, M.J. and Komives, E.A. 1999. Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation. J. Biomol. NMR 13:149-159.
45. Yurugi-Kobayashi, T., Asada, H., Shiroishi, M., Shimamura, T., Funamoto, S., Katsuta, N., Ito, K., Sugawara, T., Tokuda, N., Tsujimoto, H., Murata, T., Nomura, N., Haga, K., Haga, T., Iwata, S., and Kobayashi, T. 2009. Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris. Biochem. Biophys. Res. Commun. 380:271-276.