Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells

International Journal of Biochemistry and Cell Biology

Volumn 39, Issue 5, 2007, Pages 931-942

  Shukla, Arun Kumar ^a   Haase, Winfried ^a   Reinhart, Christoph ^a   Michel, Hartmut ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

Author keywords

BHK cells; Glycosylation; GPCR; Localization; Neuromedin receptor; Overexpression; Pichia pastoris

Indexed keywords

HISTIDINE; HYBRID PROTEIN; NEUROMEDIN U; NEUROMEDIN U TYPE 2 RECEPTOR; RECOMBINANT RECEPTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIUM; BIOTINYLATION; CELL CULTURE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DEGLYCOSYLATION; DRUG MECHANISM; GENE OVEREXPRESSION; HAMSTER; HETEROLOGOUS EXPRESSION; IMMUNOGOLD LABELING; MAMMAL CELL; METHYLOTROPHIC YEAST; NONHUMAN; PICHIA PASTORIS; PROTEIN BINDING; PROTEIN DOMAIN; SEMLIKI FOREST ALPHAVIRUS; WILD TYPE;

ANIMALS; BLOTTING, WESTERN; CELL MEMBRANE; CELLS, CULTURED; CRICETINAE; DIMETHYL SULFOXIDE; GENE EXPRESSION; GENETIC VECTORS; GLYCOSYLATION; IODINE RADIOISOTOPES; KINETICS; MEMBRANE PROTEINS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, TRANSMISSION; NEUROPEPTIDES; PICHIA; PROTEIN BINDING; RECEPTORS, NEUROTRANSMITTER; RECOMBINANT PROTEINS;

CRICETINAE; MAMMALIA; PICHIA PASTORIS; SEMLIKI FOREST VIRUS;

EID: 34147097998     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biocel.2007.01.016     Document Type: Article

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