Production of large quantifies of isotopically labeled protein in Pichia pastoris by fermentation

Journal of Biomolecular NMR

Volumn 13, Issue 2, 1999, Pages 149-159

  Wood, Matthew J ^a   Komives, Elizabeth A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

(15NH4)2SO4; 13C glucose; Disulfide bond; Glycosylation; Protein expression

Indexed keywords

AMMONIUM SULFATE; CARBON 13; GLUCOSE; GLYCEROL; NITROGEN 15; THROMBOMODULIN; CARBON; EPIDERMAL GROWTH FACTOR; NITROGEN; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN;

ARTICLE; CELL DENSITY; FERMENTATION; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN SYNTHESIS; AMINO ACID SEQUENCE; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MYCOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PICHIA; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN PROCESSING; PROTEIN TERTIARY STRUCTURE; TEMPERATURE;

AMINO ACID SEQUENCE; AMMONIUM SULFATE; CARBON; CARBON ISOTOPES; CULTURE MEDIA; EPIDERMAL GROWTH FACTOR; FERMENTATION; GLUCOSE; GLYCEROL; GLYCOSYLATION; ISOTOPE LABELING; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MYCOLOGY; NITROGEN; NITROGEN ISOTOPES; PEPTIDE FRAGMENTS; PICHIA; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; TEMPERATURE; THROMBOMODULIN;

EID: 0032587512     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008398313350     Document Type: Article

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