Engineering of an anti-epidermal growth factor receptor antibody to single chain format and labeling by sortase A-mediated protein ligation

Biotechnology and Bioengineering

Volumn 109, Issue 6, 2012, Pages 1461-1470

  Madej, Mariusz P ^a   Coia, Gregory ^a   Williams, Charlotte C ^a   Caine, Joanne M ^a   Pearce, Lesley A ^a   Attwood, Rebecca ^a   Bartone, Nick A ^a   Dolezal, Olan ^a   Nisbet, Rebecca M ^a   Nuttall, Stewart D ^a   Adams, Timothy E ^a  

^a CSIRO   (Australia)

Author keywords

Bioconjugation; Biosensor; EGFR; Enzyme mediated ligation; ScFv

Indexed keywords

ACCEPTOR MOLECULES; BIO-CONJUGATION; CARCINOMA CELLS; CELL WALLS; CLINICAL SETTINGS; CONJUGATION SYSTEM; EGFR; ENZYME-MEDIATED LIGATION; EPIDERMAL GROWTH FACTOR RECEPTORS; EXTRACELLULAR DOMAINS; FLOW CELLS; GROWTH FACTOR; HUMAN TUMORS; IN-SITU; NEGATIVE CONTROL; PEPTIDE RECOGNITION; PROTEIN LIGATION; RECOMBINANT ANTIBODY; SCFV; SINGLE CHAINS; SINGLE-CHAIN ANTIBODIES; SITE-SPECIFIC; SORTASE; STAPHYLOCOCCUS AUREUS; THREE-COMPONENT SYSTEM;

AMINO ACIDS; BACTERIA; BIOSENSORS; COENZYMES; CYTOLOGY; DISEASES; GENETIC ENGINEERING; MONOCLONAL ANTIBODIES; TISSUE;

CELLS;

EPIDERMAL GROWTH FACTOR RECEPTOR; EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY; MONOCLONAL ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; SORTASE; SORTASE A; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTIBODY ENGINEERING; ANTIBODY LABELING; ARTICLE; BIOSENSOR; CARBOXY TERMINAL SEQUENCE; CONJUGATION; CONTROLLED STUDY; DRUG CONJUGATION; ESCHERICHIA COLI; MOLECULAR RECOGNITION; MOUSE; NONHUMAN; PROTEIN DOMAIN; PROTEIN EXPRESSION; SORTASE A MEDIATED PROTEIN LIGATION; STAPHYLOCOCCUS AUREUS;

AMINOACYLTRANSFERASES; ANTIBODIES, BLOCKING; BACTERIAL PROTEINS; BIOTIN; CELL LINE, TUMOR; CYSTEINE ENDOPEPTIDASES; HUMANS; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES; STAINING AND LABELING;

STAPHYLOCOCCUS AUREUS;

EID: 84859891859     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24407     Document Type: Article

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