Nonspecific amine immobilization of ligand can be a potential source of error in BIAcore binding experiments and may reduce binding affinities

Analytical Biochemistry

Volumn 253, Issue 1, 1997, Pages 103-111

  Kortt, Alexander A ^a   Oddie, Geoffrey W ^a   Iliades, Peter ^a   Gruen, L Clem ^a   Hudson, Peter J ^a  

^a CSIRO   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BINDING ENERGY;

AMINE IMMOBILIZATION; ANTIBODY FRAGMENT; BINDING AFFINITIES; DIRECTED IMMOBILIZATION; KINETIC MEASUREMENT; POTENTIAL PROBLEMS; POTENTIAL SOURCES; SOLUTION EQUILIBRIUM;

ANTIBODIES;

IMMUNOGLOBULIN F(AB) FRAGMENT;

ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BIOSENSOR; IMMOBILIZATION; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

EID: 0031282109     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1997.2333     Document Type: Article

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