Rapid and reliable cloning of antibody variable regions and generation of recombinant single chain antibody fragments

Tissue Antigens

Volumn 47, Issue 1, 1996, Pages 1-20

  Gilliland, L K ^a,b   Norris, N A ^a   Marquardt, H ^a   Tsu, T T ^a   Hayden, M S ^a   Neubauer, M G ^a   Yelton, D E ^a   Mittler, R S ^a   Ledbetter, J A ^a  

^a BRISTOL MYERS SQUIBB   (United States)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Cloning; COS; Expression mammalian; Single chain; V region

Indexed keywords

ANTIBODY; CD2 ANTIGEN; CD28 ANTIGEN; CD3 ANTIGEN; CD4 ANTIGEN; CD40 ANTIGEN; CD45 ANTIGEN; CD8 ANTIGEN; CELL SURFACE RECEPTOR; HYBRID PROTEIN; IMMUNOGLOBULIN G1; IMMUNOTOXIN; LEUKOCYTE ANTIGEN; RECOMBINANT PROTEIN; SINGLE CHAIN ANTIBODY VARIABLE REGION FRAGMENT; T LYMPHOCYTE RECEPTOR; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; CELLULAR DISTRIBUTION; CONSENSUS SEQUENCE; CONTROLLED STUDY; DNA FLANKING REGION; DNA TRANSFECTION; DRUG CLEARANCE; DRUG PENETRATION; EXPRESSION VECTOR; GENE EXPRESSION; GENE THERAPY; HUMAN; HUMAN CELL; HYBRID CELL; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RAT; T LYMPHOCYTE;

EID: 0030026795     PISSN: 00012815     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1399-0039.1996.tb02509.x     Document Type: Article

References (79)

1. Bird RE, Hardman KD, Joacobson JW. Single-chain antigen-binding proteins. Science 1988: 242: 423-426.
2. Huston JS, Levinson D, Mudgett-Hunter M et al. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci U S A 1988: 85: 5879-5883.
3. Colcher D, Bird R, Roselli M et al. In vivo tumor targeting of a recombinant single-chain antigen-binding protein. J Natl Cancer Inst 1990: 82: 1191-1197.
4. Yokota T, Milenic DE, Whitlow M, Schlom J. Rapid tumor penetration of a single chain Fv and comparison with other immunoglobulin forms. Cancer Res 1992: 52: 3402-3408.
5. Traunecker A, Lanzavecchia A, Karjalainen K. Bispecific single chain molecules (Janusins) target cytotoxic lymphocytes on HIV infected cells. EMBO J 1991: 10: 3655-3659.
6. Hayden MS, Linsley PS, Gayle MG et al. Single-chain mono- and bispecific antibody derivatives with novel biological properties and anti-tumor activity from a COS cell transient expression system. Ther Immunol 1994: 1: 3-15.
7. Gruber M, Schodin BA, Wilson ER, Kranz DM. Efficient tumor cell lysis mediated by a bispecific single chain antibody expressed in Escherichia coli. J Immunol 1994: 152: 5368-5374.
8. Kurucz I, Titus JA, Jost CR, Jacobus CM, Segal DM. Retargeting of CTL by an efficiently refolded bispecific single-chain Fv dimer produced in bacteria. J Immunol 1995: 154: 4576-4582.
9. Batra JK, FitzGerald DJ, Gately M, Chaudhary VK, Pastan I. Anti-Tac(Fv)-PE40, a single chain antibody Pseudomonas fusion protein directed at interleukin 2 receptor bearing cells. J Biol Chem 1990: 265: 15198-15202.
10. Kreitman RJ, Chaudhary VK, Waldmann T, Willingham MC, FitzGerald DJ, Pastan I. The recombinant immunotoxin anti-Tac(Fv)-Pseudomonas exotoxin 40 is cytotoxic toward peripheral blood malignant cells from patients with adult T-cell leukemia. Proc Natl Acad Sci U S A 1990: 87: 8291-8295.
11. Brinkmann U, Pai LH, FitzGerald DJ, Willingham M, Pastan I. B3 (Fv)-PE38KDEL, a single-chain immunotoxin that causes complete regression of a human carcinoma in mice. Proc Natl Acad Sci U S A 1991: 88: 8616-8620.
12. Francisco JA, Gilliland LK, Stebbins MR, Norris NA, Ledbetter JA, Siegall CB. Activity of a single-chain immunotoxin that selectively kills lymphoma and other B-lineage cells expressing the CD40 antigen. Cancer Res 1995: 55: 3099-3104.
13. Jones PT, Dear PH, Foote J, Neuberger MS, Winter G. Replacing the complementarity-determining regions in a human antibody with those from a mouse. Nature 1986: 321: 522-525.
14. Verhoeyen M, Milstein C, Winter G. Reshaping human antibodies: grafting an anti-lysozyme activity. Science 1988: 239: 1534-1536.
15. Riechmann L, Clark M, Waldmann H, Winter G. Reshaping human antibodies for therapy. Nature 1988: 332: 323-327.
16. Queen C, Schneider WP, Selick HE et al. A humanized antibody that binds to the interleukin 2 receptor. Proc Natl Acad Sci U S A 1989: 86: 10029-10033.
17. Tempest PR, Bremner P, Lambert M. Reshaping a human monoclonal antibody to inhibit human respiratory syncitial virus infection in vivo. Biotechnology 1991: 9: 266-271.
18. Roguska MA, Pedersen JT Keddy CA et al. Humanization of murine monoclonal antibodies through variable domain resurfacing. Proc Natl Acad Sci U S A 1994: 91: 969-973.
19. Werge TM, Baldari CT, Telford JL. Intracellular single chain Fv antibody inhibits Ras activity in T-cell antigen receptor stimulated Jurkat cells. FEBS Lett 1994: 351: 393-396.
20. Marasco WA, Haseltine WA, Chen SY. Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc Natl Acad Sci USA 1993: 90: 7889-7893.
21. Duan L, Bagasra O, Laughlin MA, Oakes JW, Pomerantz RJ. Potent inhibition of human immunodeficiency virus type 1 replication by an intracelluar anti-Rev single chain antibody. Proc Natl Acad Sci U S A 1994: 91: 5075-5079.
22. Cabilly S, Riggs AD. lmmunoglobulin transcripts and molecular history of a hybridoma that produces antibody to carcinoembryonic antigen. Gene 1985: 40: 157-161.
23. Carroll WL, Mendel E, Levy S. Hybridoma fusion cell lines contain an aberrant kappa transcript. Mol Immunol 1988: 25: 991-995.
24. Hamlyn PH, Gait MJ, Milstein C. Complete sequence of an immunoglobulin mRNA using specific priming and the dideoxynucleotide method of RNA sequencing. Nucleic Acids Res 1981: 9: 4485-4494.
25. Darveau RP, Somerville JE, Fell HP. Expression of antibody fragments in Escherichia coli. J Clin Immunoassay 1992: 15: 25-29.
26. Hellstrom I, Horn D, Linsley P, Brown JP, Brankovan V, Hellstrom KE. Monoclonal mouse antibodies raised against human lung carcinoma. Cancer Res 1986: 46: 3917-3923.
27. Field J, Mikawa J, Broek D et al. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol 1988: 8: 2159-2165.
28. Chaudhary VK, Batra JK, Gallo MG, Willingham MC, FitzGerald DJ, Pastan I. A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins. Proc Natl Acad Sci U S A 1990: 87: 1066-1070.
29. Gilman M. Preparation of cytoplasmic RNA from tissue culture cells. In: Ausubel FM, Brent R, Kingston RE et al., ed. Current protocols in molecular biology. New York: Greene Publishing Associates and Wiley-Interscience, 1990.
30. Loh EY, Elliot JF, Cwirla S, Lanier LL, Davis MM. Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain. Science 1989: 243: 217-220.
31. Huston JS, Mudgett-Hunter M, Tai MS. Protein engineering of single-chain Fv analogs and fusion proteins. Methods Enzymol 1991: 203: 46-88.
32. Seed B. An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2. Nature 1987: 329: 840-842.
33. Aruffo A, Seed B. Molecular cloning of a CD28 cDNA by a high efficiency COS cell expression system. Proc Natl Acad Sci U S A 1987: 84: 8573-8577.
34. Linsley PS, Brady W, Grosmaire L, Aruffo A, Damle NK, Ledbetter JA. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. J Exp Med 1991: 173: 721-730.
35. Rabinovitch PS, June CH, Grossmann A, Ledbetter JA. Heterogeneity amoung T cells in intracellular free calcium responses after mitogen stimulation with PHA or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry. J Immunol 1986: 137: 952-961.
36. Kabat EA, Wu TT, Perry HM, Gottesman KS, Foeller C. Sequences of proteins of immunological interest. 5th edn. Bethesda, MD: Public Health Service, National Institutes of Health, 1991.
37. Hollenbaugh D, Grosmaire LS, Kullas CD et al. The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity. EMBO J 1992: 11: 4313-4321.
38. Turka LA, Linsley PS, Paine R, Schieven GL, Thompson CB, Ledbetter JA. Signal transduction via CD4, CD8, and CD28 in mature and immature thymocytes: Implications for thymic selection. J Immunol 1991: 146: 1428-1436.
39. Ledbetter JA, Norris NA, Grossmann A et al. Enhanced transmembrane signalling activity of monoclonal antibody heteroconjugates suggests molecular interactions between receptors on the T cell surface. Mol Immunol 1989: 26: 137-145.
40. Desplancq D, King DJ, Lawson AD, Mountain A. Multimerization behavior of single chain Fv variants for the tumor-binding antibody B72.3. Protein Eng 1994: 7: 1027-1033.
41. Whitlow M, Filpula D, Rollence ML, Feng SL, Wood JF. Multivalent Fvs: characterization of single-chain Fv oligomers and preparation of a bispecific Fv. Protein Eng 1994: 7: 1017-1026.
42. Valitutti S, Muller S, Cella M, Padovan E, Lanzavecchia A. Serial triggering of many T-cell receptors by a few peptide-MHC complexes. Nature 1995: 375: 148-151.
43. Tavladoraki P, Benvenuto E, Trinca S, DeMartinis D, Cattaneo A, Galeffi P. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 1993: 366: 469-472.
44. Somia NV, Zoppe M, Verma IM. Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery. Proc Natl Acad Sci U S A 1995: 92: 7570-7574.
45. Chu TH, Dornburg R. Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. J Virol 1995: 69: 2659-2663.
46. Selsing E, Miller J, Wilson R, Storb U. Evolution of mouse immunoglobulin lambda genes. Proc Natl Acad Sci U S A 1982: 79: 4681-4685.
47. Steen ML, Hellman L, Pettersson U. The immunoglobulin lambda locus in rat consists of two C lambda genes and a single V lambda gene. Gene 1987: 55: 75-84.
48. Gavilondo-Cowley JV, Coloma MJ et al. Specific amplification of rearranged immunoglobulin variable region genes from mouse hybridoma cells. Hybridoma 1990: 9: 407-417.
49. Orlandi R, Gussow DH, Jones PT, Winter G. Cloning of immunoglobulin variable domains for expression by the polymerase chain reaction. Proc Natl Acad Sci U S A 1989: 86: 3833.
50. Larrick JW, Wallace EF, Coloma MJ, Bruderer U, Lang AB, Fry KE. Therapeutic human antibodies derived from PCR amplification of B-cell variable regions. Immunol Rev 1992: 130: 69-85.
51. Larrick JW, Danielson L, Brenner C. Polymerase chain reaction using mixed primers: cloning of human monoclonal antibody variable region genes from single hybridoma cells. Biotechnology 1989: 7: 934.
52. Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol 1976: 6: 511-519.
53. Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975: 256: 495-497.
54. Kutemeier G, Harloff C, Mocikat R. Rapid isolation of immunoglobulin variable genes from cell lysates of rat hybridomas by polymerase chain reaction. Hybridoma 1992: 11: 23-32.
55. Cabilly S. Growth at sub-optimal temperatures allows the production of functional, antigen-binding Fab fragments in Escherichia coli. Gene 1989: 85: 553-557.
56. Buchner J, Rudolph R. Renaturation, purification and characterization of recombinant Fab fragments in Escherichia coli. Biotechnology 1991: 9: 157-162.
57. Better M, Chang CP, Robinson RR, Horwitz AH. Escherichia coli secretion of an active chimeric antibody fragment. Science 1988: 240: 1041-1043.
58. Skerra A, Pluckthun A. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 1988: 240: 1038-1041.
59. Pluckthun A, Skerra A. Expression of functional antibody Fv and Fab fragments in Escherichia coli. Methods Enzymol 1989: 178: 497-515.
60. Whittle N, Adair J, Lloyd C et al. Expression in COS cells of a mouse-human chimaeric B72.3 antibody. Protein Eng 1987: 1: 499-505.
61. Cattaneo A, Neuberger MS. Polymeric immunoglobulin M is secreted by transfectants of non-lymphoid cells in the absence of immunoglobulin J chain. EMBO J 1987: 6: 2753-2758.
62. Colcher D, Milenic D, Roselli M et al. Characterization and biodistribution of recombinant and recombinant/ chimeric constructs of monoclonal antibody B72.3. Cancer Res 1989: 49: 1738-1745.
63. Horwitz AH, Chang CP, Better M, Hellstrom KE, Robinson RR. Secretion of functional antibodies and Fab fragment from yeast cells. Proc Natl Acad Sci U S A 1988: 85: 8678-8682.
64. Better M, Horwitz AH. Expression of engineered antibodies and antibody fragments in microorganisms. Methods Enzymol 1989: 178: 476-496.
65. Hasemann CA, Capra JD. High-level production of a functional immunoglobulin heterodimer in a baculovirus expression system. Proc Natl Acad Sci U S A 1990: 87: 3942-3946.
66. Potter KN, Li Y, Capra JD. Antibody production in the baculovirus expression system. Int Rev Immunol 1993: 10: 103-112.
67. Kukuruzinska MA, Bergh MLE, Kackson BJ. Protein glycosylation in yeast. Annu Rev Biochem 1987: 915-944.
68. Mallender WD, Voss Jr. EW. Construction, expression and activity of a bivalent bispecific single-chain antibody. J Biol Chem 1995: 269: 199-206.
69. Kabat EA, Wu TT, Reid-Miller M, Perry HM, Gottesman KS. Sequences of proteins of immunological interest. 4th edn. Bethesda, MD: Public Health Service, National Institutes of Health, 1987.
70. Altenburger W, Neumaier P, Steinmetz M, Zachau HG. DNA sequence of the constant gene region of the mouse immunoglobulin kappa chain. Nucleic Acids Res 1981: 9: 971-981.
71. Honjo T, Obata M, Yamawaki-Katoaka Y et al. Cloning and complete nucleotide sequence of mouse immunoglobulin γ1 chain gene. Cell 1979: 18: 559-568.
72. Ollo R, Auffray C, Morchamps C, Rougeon F. Comparison of mouse immunoglobulin γ2a and γ2b chain genes suggests that exons can be exchanged between genes in a multigenic family. Proc Natl Acad Sci U S A 1981: 78: 2442-2446.
73. Tucker PW, Marcu KB, Newell N, Richards J, Blattner FR. Sequence of the cloned gene for the constant region of murine γ2b immunoglobulin heavy chain. Science 1979: 206: 1303-1306.
74. Wels JA, Word CJ, Rimm D. Structural analysis of the murine IgG3 constant region gene. EMBO J 1984: 3: 2041-2046.
75. Sheppard HW, Gutman GA. Allelic forms of rat kappa chain genes: evidence for strong selection at the level of nucleotide sequence. Proc Natl Acad Sci U S A 1981: 78: 7064-7068.
76. Bruggemann M. Evolution of the rat immunoglobulin gamma heavy-chain gene family. Gene 1988: 74: 473-482.
77. Bruggemann M, Delmastro-Galfre P, Waldmann H, Calabi F. Sequence of a rat immunoglobulin γ2c heavy chain constant region cDNA: extensive homology to mouse γ3. Eur J Immunol 1988: 18: 317-319.
78. Diegel ML, Moran PA, Gilliland LK et al. Regulation of HIV production by blood mononuclear cells from HIV-infected donors. II. HIV-1 production depends on T cell-monocyte interaction. AIDS Res Hum Retroviruses 1993: 9: 465-473.
79. Linsley PS, Brady W, Urnes M, Grosmaire LS, Damle NK, Ledbetter JA. CTLA-4 is a second receptor for the B cell activation antigen B7. J Exp Med 1991: 174: 561-569.