The Corynebacterium diphtheriae shaft pilin SpaA is built of tandem Ig-like modules with stabilizing isopeptide and disulfide bonds

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 40, 2009, Pages 16967-16971

  Hae, Joo Kang ^a   Paterson, Neil G ^a   Gaspar, Andrew H ^b   Hung, Ton That ^b,c   Baker, Edward N ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

^c UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

Bacterial pilus; Crystal structure; Mass spectrometry; Pilin motif; Polymerization

Indexed keywords

ADHESIN; ASPARAGINE; BACTERIAL PROTEIN; CALCIUM ION; DISULFIDE; IMMUNOGLOBULIN; ISOPEPTIDE; LYSINE; MONOMER; PEPTIDE DERIVATIVE; PILIN; SPAA PROTEIN; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS CEREUS; CONTROLLED STUDY; CORYNEBACTERIUM DIPHTHERIAE; CROSS LINKING; CRYSTAL STRUCTURE; DISULFIDE BOND; GENETIC ANALYSIS; GRAM POSITIVE BACTERIUM; MASS SPECTROMETRY; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN TERTIARY STRUCTURE; STREPTOCOCCUS PYOGENES;

AMINO ACID SEQUENCE; ASPARAGINE; BACTERIAL PROTEINS; CORYNEBACTERIUM DIPHTHERIAE; CRYSTALLOGRAPHY, X-RAY; DISULFIDES; FIMBRIAE PROTEINS; IMMUNOGLOBULINS; LYSINE; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; THREONINE;

BACILLUS CEREUS; BACTERIA (MICROORGANISMS); CORYNEBACTERIUM DIPHTHERIAE; POSIBACTERIA; STREPTOCOCCUS PYOGENES;

EID: 70350133602     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0906826106     Document Type: Article

References (39)

1. Ton-That H, Schneewind O (2004) Assembly of pili in Gram-positive bacteria. Trends Microbiol 12:228-234.
2. Proft T, Baker EN (2009) Pili in Gram-negative and Gram-positive bacteria - structure, assembly and their role in disease. Cell Mol Life Sci 66:613-635.
3. Telford JL, Barocchi MA, Margarit I, Rappuoli R, Grandi G (2006) Pili in gram-positive pathogens. Nat Rev Microbiol 4:509-519.
4. Budzik JM, et al. (2008) Amide bonds assemble pili on the surface of bacilli. Proc Natl Acad Sci USA 105:10215-10220.
5. Kang HJ, Coulibaly F, Clow F, Proft T, Baker EN (2007) Stabilizing isopeptide bonds revealed in Gram-positive bacterial pilus structure. Science 318:1625-1628. (Pubitemid 350233661)
6. Ton-That H, Schneewind O (2003) Assembly of pili on the surface of Corynebacterium diphtheriae. Mol Microbiol 50:1429-1438.
7. Ton-That H, Marraffini LA, Schneewind O (2004) Sortases and pilin elements involved in pilus assembly of Corynebacterium diphtheriae. Mol Microbiol 53:251-261.
8. Gaspar AH, Ton-That H (2006) Assembly of distinct pilus structures on the surface of Corynebacterium diphtheriae. J Bacteriol 188:1526-1533.
9. Mandlik A, Das A, Ton-That H (2008) The molecular switch that activates the cell wall anchoring step of pilus assembly in Gram-positive bacteria. Proc Natl Acad Sci USA 105:14147-14152.
10. Swierczynski A, Ton-That H (2006) Type III pilus of corynebacteria: Pilus length is determined by the level of its major pilin subunit. J Bacteriol 188:6318-6325. (Pubitemid 44448574)
11. Swaminathan A, et al. (2007) Housekeeping sortase facilitates the cell wall anchoring of pilus polymers in Corynebacterium diphtheriae. Mol Microbiol 66:961-974.
12. Budzik JM, Oh SY, Schneewind O (2009) Sortase D forms the covalent bond that links BcpB to the tip of Bacillus cereus pili. J Biol Chem 284:12989-12997.
13. Deivanayagam CC, et al. (2000) Novel fold and assembly of the repetitive B region of the Staphylococcus aureus collagen-binding surface protein. Structure 8:67-78.
14. Symersky J, et al. (1997) Structure of the collagen-binding domain from a Staphylococcus aureus adhesin. Nat Struct Biol 4:833-838. (Pubitemid 27435349)
15. Krishnan V, et al. (2007) An IgG-like domain in the minor pilin GBS52 of Streptococcus agalactiae mediates lung epithelial cell adhesion. Structure 15:893-903. (Pubitemid 47212752)
16. Deivanayagam CC, et al. (2002) A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: Crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A. EMBO J 21:6660-6672.
17. Liu Q, et al. (2007) The Enterococcus faecalis MSCRAMM ACE binds its ligand by the collagen hug model. J Biol Chem 282:19629-19637. (Pubitemid 47100065)
18. Wikoff WR, et al. (2000) Topologically linked protein rings in the bacteriophage HK97 capsid. Science 289:2129-2133.
19. Barocchi MA, et al. (2006) A pneumococcal pilus influences virulence and host inflammatory responses. Proc Natl Acad Sci USA 103:2857-2862.
20. Budzik JM, Schneewind O (2006) Pili prove pertinent to enterococcal endocarditis. J Clin Invest 116:2582-2584.
21. Lauer P, et al. (2005) Genome analysis reveals pili in Group B Streptococcus. Science 309:105. (Pubitemid 40934983)
22. Patti JM, Allen BL, McGavin MJ, Hook M (1994) MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol 48:585-617. (Pubitemid 2157780)
23. Rich RL, et al. (1998) Domain structure of the Staphylococcus aureus collagen adhesin. Biochemistry 37:15423-15433. (Pubitemid 28516004)
24. Kang HJ, Baker EN (2009) Intramolecular isopeptide bonds give thermodynamic and proteolytic stability to the major pilin protein of Streptococcus pyogenes. J Biol Chem, in press.
25. Dutton RJ, et al. (2008) Bacterial species exhibit diversity in their mechanisms and capacity for protein disulfide bond formation. Proc Natl Acad Sci USA 105:11933-11938.
26. Heras B, et al. (2009) DSB proteins and bacterial pathogenicity. Nat Rev Microbiol 7:215-225.
27. Yeates TO, Clubb RT (2007) How some pili pull. Science 318:1558-1559.
28. Sokurenko EV, Vogel V, Thomas WE (2008) Catch-bond mechanism of force-enhanced adhesion: counterintuitive, elusive, but...widespread? Cell Host Microbe 4:314-323.
29. Zong Y, et al. (2005) A 'Collagen Hug' model for Staphylococcus aureus CNA binding to collagen. EMBO J 24:4224-4236.
30. Kang HJ, Paterson NG, Baker EN (2009) Expression, purification, crystallization and preliminary crystallographic analysis of SpaA, a major pilin from Corynebacterium diphtheriae. Acta Crystallogr Sect F Struct Biol Cryst Commun, in press.
31. VanDuyne GD, Standaert RF, Karplus PA, Schreiber SL, Clardy J (1993) Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin. JMol Biol 229:105-124. (Pubitemid 23037917)
32. Collaborative Computational Project, Number 4 (1994) The CCP4 suite: Programs for protein crystallography. Acta Crystallogr D Biol Crystallogr 50:760-763.
33. Schneider TR, SheldrickGM(2002) Substructure solution with SHELXD. Acta Crystallogr D Biol Crystallogr 58:1772-1779.
34. Vonrhein C, Blanc E, Roversi P, Bricogne G (2007) Automated structure solution with autoSHARP. Methods Mol Biol 364:215-230.
35. Adams PD, et al. (2004) Recent developments in the PHENIX software for automated crystallographic structure determination. J Synchrotron Radiat 11:53-55. (Pubitemid 40085633)
36. Terwilliger TC (2003) Automated main-chain model building by template matching and iterative fragment extension. Acta Crystallogr D Biol Crystallogr 59:38-44. (Pubitemid 36117204)
37. Emsley P, Cowtan K (2004) Coot: Model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60:2126-2132. (Pubitemid 41742764)
38. Murshudov GN, Vagin AA, Dodson EJ (1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr 53:240-255.
39. Krissinel E, Henrick K (2004) Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions. Acta Crystallogr D Biol Crystallogr 60:2256-2268. (Pubitemid 41742778)