Protein-Protein Fusion Catalyzed by Sortase A

PLoS ONE

Volumn 6, Issue 4, 2011, Pages

  Levary, David A ^b   Parthasarathy, Ranganath ^c   Boder, Eric T ^c   Ackerman, Margaret E ^a  

^a DARTMOUTH COLLEGE   (United States)

^b HARVARD UNIVERSITY   (United States)

^c UNIVERSITY OF TENNESSEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; CARCINOEMBRYONIC ANTIGEN; GELONIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; IMMUNOGLOBULIN G; SORTASE; SORTASE A; UNCLASSIFIED DRUG; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME BINDING; ENZYME MECHANISM; IN VITRO STUDY; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN HYDROLYSIS; REACTION OPTIMIZATION; AMINO ACID SEQUENCE; BIOCATALYSIS; CELL STRAIN HEK293; CHEMISTRY; HUMAN; METABOLISM; MOLECULAR GENETICS; PROTEIN MOTIF; PROTEIN TERTIARY STRUCTURE;

BACTERIA (MICROORGANISMS);

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; BIOCATALYSIS; CYSTEINE ENDOPEPTIDASES; HEK293 CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

EID: 79953733187     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0018342     Document Type: Article

References (28)

1. Dawson PE, Kent SB, (2000) Synthesis of native proteins by chemical ligation. Annu Rev Biochem 69: 923-960.
2. Blaschke UK, Silberstein J, Muir TW, (2000) Protein engineering by expressed protein ligation. Methods Enzymol 328: 478-496.
3. Jackson DY, Burnier J, Quan C, Stanley M, Tom J, et al. (1994) A designed peptide ligase for total synthesis of ribonuclease A with unnatural catalytic residues. Science 266: 243-247.
4. Ayers B, Blaschke UK, Camarero JA, Cotton GJ, Holford M, et al. (1999) Introduction of unnatural amino acids into proteins using expressed protein ligation. Biopolymers 51: 343-354.
5. Bentley ML, Lamb EC, McCafferty DG, (2008) Mutagenesis studies of substrate recognition and catalysis in the sortase A transpeptidase from Staphylococcus aureus. J Biol Chem 283: 14762-14771.
6. Mazmanian SK, Liu G, Ton-That H, Schneewind O, (1999) Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science 285: 760-763.
7. Mao H, Hart SA, Schink A, Pollok BA, (2004) Sortase-mediated protein ligation: a new method for protein engineering. J Am Chem Soc 126: 2670-2671.
8. Popp MW, Antos JM, Grotenbreg GM, Spooner E, Ploegh HL, (2007) Sortagging: a versatile method for protein labeling. Nat Chem Biol 3: 707-708.
9. Tanaka T, Yamamoto T, Tsukiji S, Nagamune T, (2008) Site-specific protein modification on living cells catalyzed by Sortase. Chembiochem 9: 802-807.
10. Pritz S, Wolf Y, Kraetke O, Klose J, Bienert M, et al. (2007) Synthesis of biologically active peptide nucleic acid-peptide conjugates by sortase-mediated ligation. J Org Chem 72: 3909-3912.
11. Samantaray S, Marathe U, Dasgupta S, Nandicoori VK, Roy RP, (2008) Peptide-sugar ligation catalyzed by transpeptidase sortase: a facile approach to neoglycoconjugate synthesis. J Am Chem Soc 130: 2132-2133.
12. Parthasarathy R, Subramanian S, Boder ET, (2007) Sortase A as a novel molecular "stapler" for sequence-specific protein conjugation. Bioconjug Chem 18: 469-476.
13. Chan L, Cross HF, She JK, Cavalli G, Martins HF, et al. (2007) Covalent attachment of proteins to solid supports and surfaces via Sortase-mediated ligation. PLoS ONE 2: e1164.
14. Clow F, Fraser JD, Proft T, (2008) Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A. Biotechnol Lett 30: 1603-1607.
15. Wu Z, Guo X, Wang Q, Swarts BM, Guo Z, (2010) Sortase A-catalyzed transpeptidation of glycosylphosphatidylinositol derivatives for chemoenzymatic synthesis of GPI-anchored proteins. J Am Chem Soc 132: 1567-1571.
16. Antos JM, Miller GM, Grotenbreg GM, Ploegh HL, (2008) Lipid Modification of Proteins through Sortase-Catalyzed Transpeptidation. J Am Chem Soc 130: 16338-16343.
17. Wu S, Proft T, (2010) The use of sortase-mediated ligation for the immobilisation of bacterial adhesins onto fluorescence-labelled microspheres: a novel approach to analyse bacterial adhesion to host cells. Biotechnol Lett 32: 1713-1718.
18. Sakamoto T, Sawamoto S, Tanaka T, Fukuda H, Kondo A, (2010) Enzyme-Mediated Site-Specific Antibody-Protein Modification Using a ZZ Domain as a Linker. Bioconjug Chem.
19. Popp MW, Dougan SK, Chuang TY, Spooner E, Ploegh HL, (2011) Sortase-catalyzed transformations that improve the properties of cytokines. Proc Natl Acad Sci U S A.
20. Refaei MA, Combs A, Kojetin DJ, Cavanagh J, Caperelli C, et al. (2011) Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy. J Biomol NMR 49: 3-7.
21. Pritz S, Wolf Y, Kraetke O, Klose J, Bienert M, et al. (2009) Enzymatic ligation of peptides, peptide nucleic acids and proteins by means of sortase A. Adv Exp Med Biol 611: 107-108.
22. Proft T, (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation. Biotechnol Lett 32: 1-10.
23. Popp MW, Antos JM, Ploegh HL, (2009) Site-specific protein labeling via sortase-mediated transpeptidation. Curr Protoc Protein Sci Chapter 15: Unit 15 13.
24. Ton-That H, Mazmanian SK, Alksne L, Schneewind O, (2002) Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Cysteine 184 and histidine 120 of sortase form a thiolate-imidazolium ion pair for catalysis. J Biol Chem 277: 7447-7452.
25. Hackel BJ, Ackerman ME, Howland SW, Wittrup KD, (2010) Stability and CDR composition biases enrich binder functionality landscapes. J Mol Biol 401: 84-96.
26. Orcutt KD, Ackerman ME, Cieslewicz M, Quiroz E, Slusarczyk AL, et al. (2010) A modular IgG-scFv bispecific antibody topology. Protein Eng Des Sel 23: 221-228.
27. Shusta EV, Raines RT, Pluckthun A, Wittrup KD, (1998) Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments. Nat Biotechnol 16: 773-777.
28. Sikorski RS, Hieter P, (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.