Thermal stabilty of glucokinase (GK) as influenced by the substrate glucose, an allosteric glucokinase activator drug (GKA) and the osmolytes glycerol and urea

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1824, Issue 5, 2012, Pages 769-784

  Zelent, B ^a   Buettger, C ^a   Grimsby, J ^b   Sarabu, R ^b   Vanderkooi, J M ^a   Wand, A J ^a   Matschinsky, F M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b Hoffman La Roche Department of Metabolic Diseases   (United States)

Author keywords

Calorimetry; Denaturation; Diabetes; Glucokinase; Protein stability; Tryptophan fluorescence

Indexed keywords

APOENZYME; ENZYME ACTIVATOR; GLUCOKINASE; GLUCOKINASE ACTIVATOR; GLUCOSE; GLYCEROL; SURFACE WATER; UNCLASSIFIED DRUG; UREA; ADENOSINE TRIPHOSPHATE; HYBRID PROTEIN; WATER;

ALLOSTERISM; ARTICLE; CONFORMATION; ENZYME BINDING; ENZYME DENATURATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; HYDROPHOBICITY; PRIORITY JOURNAL; THERMOSTABILITY; WILD TYPE; CHEMICAL STRUCTURE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; KINETICS; MUTATION; OSMOTIC PRESSURE; PROTEIN CONFORMATION; PROTEIN FOLDING; TEMPERATURE; THERMODYNAMICS;

ANIMALIA;

ADENOSINE TRIPHOSPHATE; ALLOSTERIC REGULATION; APOENZYMES; ENZYME ACTIVATORS; ENZYME STABILITY; ESCHERICHIA COLI; GLUCOKINASE; GLUCOSE; GLYCEROL; HUMANS; KINETICS; MODELS, MOLECULAR; MUTATION; OSMOTIC PRESSURE; PROTEIN CONFORMATION; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; TEMPERATURE; THERMODYNAMICS; UREA; WATER;

EID: 84859779953     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2012.03.003     Document Type: Article

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