Sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence

Biochemical Journal

Volumn 413, Issue 2, 2008, Pages 269-280

  Zelent, Bogumil ^a   Odili, Stella ^a   Buettger, Carol ^a   Shiota, Chiyo ^b   Grimsby, Joseph ^c   Taub, Rebecca ^c   Magnuson, Mark A ^b   Vanderkooi, Jane M ^a   Matschinsky, Franz M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Hoffman La Roche Department of Metabolic Diseases   (United States)

Author keywords

Allosteric activation; Equilibrium binding; Glucokinase; Glucokinase activator drugs (GKA drugs); Glucokinase disease (GK disease); Protein conformation; Tryptophan fluorescence

Indexed keywords

ACIDS; AMINES; ENZYME KINETICS; ENZYMES; FLUORESCENCE; FOOD ADDITIVES; GLUCOSE; LIGHT EMISSION; LUMINESCENCE; ORGANIC ACIDS; QUANTUM YIELD; SUGAR (SUCROSE); SUGARS; SYSTEM THEORY;

BINDING CONSTANTS; BINDING STUDIES; D-GLUCOSE; DIABETES MELLITUS (DM); FLUORESCENCE QUANTUM YIELD (FQY); GLUCOKINASE (GCK); GLUTATHIONE TRANSFERASE (GST); HOMOEOSTASIS; HYPOGLYCAEMIA; INDIVIDUAL (PSS 544-7); KINETIC MEASUREMENTS; N ACETYL D GLUCOSAMINE (GLCNAC); SUGAR BINDING; TRYPTOPHAN FLUORESCENCE; TRYPTOPHAN RESIDUES; WILD-TYPE (WT);

AMINO ACIDS;

AMINO ACID DERIVATIVE; DEOXYGLUCOSE; DEXTRO MANNO HEPTULOSE; GLUCOKINASE; GLUCOSE; GLUTATHIONE TRANSFERASE; LEVO GLUCOSE; MANNOHEPTULOSE; MANNOSE; MUTANT PROTEIN; N ACETYLGLUCOSAMINE; RECOMBINANT PROTEIN; SUGAR; TRYPTOPHAN; CARBOHYDRATE; HYBRID PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CATALYSIS; CONTROLLED STUDY; ENZYME KINETICS; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN PURIFICATION; BINDING SITE; CHEMISTRY; CONFORMATION; ESCHERICHIA COLI; GENETICS; KINETICS; METABOLISM; METHODOLOGY; MUTATION; PROTEIN BINDING; SPECTROFLUOROMETRY;

BINDING SITES; CARBOHYDRATES; ESCHERICHIA COLI; GLUCOKINASE; GLUTATHIONE TRANSFERASE; HUMANS; KINETICS; MOLECULAR CONFORMATION; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; TRYPTOPHAN;

EID: 48149093497     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20071718     Document Type: Article

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