Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography

Separation and Purification Technology

Volumn 90, Issue , 2012, Pages 133-139

  Santos, Maria João ^a   Teixeira, José A ^a   Rodrigues, Lígia R ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Anion exchange chromatography; Isolation; Separation; Whey proteins

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; BOVINE SERUM ALBUMINS; FAST PROTEIN LIQUID CHROMATOGRAPHY; ISOLATION; LACTOGLOBULIN; SALT GRADIENT; WHEY PROTEIN CONCENTRATE; WHEY PROTEINS;

FRACTIONATION; IONIC STRENGTH; IONS; LIQUID CHROMATOGRAPHY; SEPARATION; SODIUM CHLORIDE;

PROTEINS;

BOVINAE;

EID: 84858727228     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2012.02.030     Document Type: Article

References (52)

1. K. Marshall Therapeutic applications of whey proteins Altern. Med. Rev. 9 2 2004 136 156
2. S. Jovanovic, M. Barac, O. Macej, T. Vucic, and C. Lacnjevac SDS-PAGE analysis of soluble proteins in reconstituted milk exposed to different heat treatments Sensors 7 2007 371 383
3. R. Hahn, P.M. Schulz, C. Schaupp, and A. Jungbauer Bovine whey fractionation based on cation-exchange chromatography J. Chromatogr. A 795 1998 277 287 (Pubitemid 28080534)
4. H.M. Farrel, R. Jimenez-Flores Jr., G.T. Bleck, J.E. Butler, L.K. Creamer, C.M. Hicks, K.F. Ng-Kwai-Hang, and H.E. Swaisgood Nomenclature of the proteins of cows' milk - sixth revision J. Dairy Sci. 87 2004 1641 1674 (Pubitemid 38893295)
5. S.J. Geberding, and C.H. Byers Preparative ion-exchange chromatography of proteins from dairy whey J. Chromatogr. A 808 1998 141 151 (Pubitemid 28273228)
6. D.R. Flower The lipocalin protein family: structure and function - review article Biochem. J. 318 1996 1 14 (Pubitemid 26283618)
7. L.H. Greene, J.A. Grobler, V.A. Malinovskii, J. Tian, K.R. Azharya, and K. Brew Stability, activity and flexibility in α-Lactalbumin Protein Eng. 12 7 1999 581 587
8. M.M. El-Loly Bovine milk immunoglobulins in relation to human health Int. J. Dairy Sci. 2 39 2007 183 195
9. L.M. Huffman, and W.J. Harper Maximizing the value of milk through separation technologies J. Dairy Sci. 82 1999 2238 2244 (Pubitemid 129552466)
10. A.L. Zydney Protein separations using membrane filtration: new opportunities for whey fractionation Int. Dairy J. 8 1998 243 250 (Pubitemid 28436837)
11. G.I. Imafidon, N.Y. Farkye, and A. Spanier Isolation, purification, and alteration of some functional groups of major milk proteins: a review Crit. Rev. Food Sci. Nutr. 37 7 1997 663 689 (Pubitemid 127348672)
12. J.N. Wit Nutritional and functional characteristics of whey proteins in food products J. Dairy Sci. 81 1998 597 608
13. J.M. Lozano, G.I. Giraldo, and C.M. Romero An improved method for isolation of β-lactoglobulin Int. Dairy J. 18 2008 55 63
14. M. Liang, V.Y.T. Chen, H. Chen, and W. Chen A simple and direct isolation of whey components from raw milk by gel filtration chromatography and structural characterization by Fourier transform Raman spectroscopy Talanta 69 2006 1269 1277 (Pubitemid 43866184)
15. R. Rossano, A. D'Elia, and P. Riccio One-step separation from lactose: recovery and purification of major cheese-whey proteins by hydroxyapatite - a flexible procedure suitable for small- and medium-scale preparations Protein Expr. Purif. 21 2001 165 169 (Pubitemid 32614510)
16. G.W. Smithers Whey and whey proteins - from 'gutter-to-gold' Int. Dairy J. 18 2008 695 704
17. S. Bhattacharjee, C. Bhattacharjee, and S. Datta Studies on the fractionation of β-lactoglobulin from casein whey using ultrafiltration and ion-exchange membrane chromatography J. Membr. Sci. 275 2006 141 150
18. S. Goodall, A.S. Grandison, P.J. Jauregi, and J. Price Selective separation of the major whey proteins using ion exchange membranes J. Dairy Sci. 91 2008 1 10 (Pubitemid 351455812)
19. E. Drioli, and M. Romano Progress and new perspectives on integrated membrane operations for sustainable industrial growth Ind. Eng. Chem. 40 2001 1277 1300
20. R. Ulber, K. Plate, T. Demmer, H. Buchholz, and T. Scheper Downstream processing of bovine lactoferrin from sweet whey Acta Biotechnol. 21 2001 27 34 (Pubitemid 32177816)
21. C. Selkirk Ion-exchange chromatography Methods Mol. Biol. 244 2004 125 131
22. I. Ali, H.Y. Aboul-Enein, P. Singh, R. Singh, and B. Sharma Separation of biological proteins by liquid chromatography: review article Saudi Pharm. J. 18 2010 59 73
23. A.C. Grodzki, and E. Berenstein Antibody purification: ion-exchange chromatography Methods Mol. Biol. 588 2010 27 32
24. D.H.G. Pelegrine, and C.A. Gasparetto Whey proteins solubility as function of temperature and pH Food Sci. Technol. 38 2005 77 80 (Pubitemid 40727789)
25. M.M. Bradford A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
26. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 221 1970 680 685
27. H. Shen, and D.D. Frey Charge regulation in protein ion-exchange chromatography: development and experimental evaluation of a theory based on hydrogen ion Donnan equilibrium J. Chromatogr. A 1034 2004 55 68 (Pubitemid 38379682)
28. T. Ishihara, and S. Yamamoto Separation behavior of proteins near the isoelectric points in electrostatic interaction (ion exchange) chromatography Prog. Biotechnol. 16 2000 93 98
29. Y. Sakakibara, and H. Yanagisawa Techniques for the separation of proteins by isoelectric point column chromatography Nat. Sci. 56 2007 45 49
30. J.H. Song, W. Kim, H. Park, E. Yu, and D.W. Lee Retention characteristics of various proteins in flow field-flow fractionation: effects of pH, ionic strength, and denaturation Bull. Korean Chem. Soc. 20 10 1999 1159 1164
31. L. Chianese, S. Caira, S. Lilla, F. Pizzolongo, P. Ferranti, G. Pugliano, and F. Addeo Primary structure of water buffalo alpha-lactalbumin variants A and B J. Dairy Res. 71 1 2004 14 19 (Pubitemid 38370810)
32. L. Barbosa, M.G. Ortore, F. Spinozzi, P. Mariani, and S. Bernstorff The importance of protein-protein interactions on the pH-induced conformational changes of bovine serum albumin: a small-angle X-ray scattering study Biophys. J. 98 2010 147 157
33. V. Levi, and F.L.G. Flecha Reversible fast-dimerization of bovine serum albumin detected by fluorescence resonance energy transfer Biochim. Biophys. Acta 1599 1-2 2002 141 148 (Pubitemid 38234522)
34. A.K. Hunter, and G. Carta Effects of bovine serum albumin heterogeneity on frontal analysis with anion-exchange media J. Chromatogr. A 93 2001 13 19 (Pubitemid 33079151)
35. W.F. Weinbrenner, and M.R. Etzel Competitive adsorption of α-Lactalbumin and bovine serum albumin to a sulfopropyl ion-exchange membrane J. Chromatogr. A 662 1994 414 419
36. G.B. van der Berg, and J.H. Hanemaaijer Ultrafiltration of protein solutions; the role of protein association in rejection and osmotic pressure J. Membr. Sci. 31 1987 307 320 (Pubitemid 17121603)
37. H.A. McKenzie, and W.H. Sawyer Effect of pH on beta-lactoglobulins Nature 214 93 1967 1101 1104
38. M. Stastna, and K. Slais Two-dimensional gel isoelectric focusing Electrophoresis 26 18 2005 3586 3591 (Pubitemid 41410381)
39. T. Cano, N.D. Offringa, and R.C. Wilson Competitive ion-exchange adsorption of proteins: competitive isotherms with controlled competitor concentration J. Chromatogr. A 1079 1-2 2005 116 126 (Pubitemid 40813193)
40. M.M.H. El-Sayed, H.A. Chase, Separation of the two major whey proteins using cation-exchange adsorption, Proceedings of the World Congress on Engineering and Computer Science, October 22-24, 2008, San Francisco, USA.
41. M.R. Aboudzadeh, N. Aboudzadeh, Z. Jiawen, and W. Bin Binary protein adsorption to DEAE sepharose FF Korean J. Chem. Eng. 24 4 2007 641 647
42. M. Fuentes, B.C. Pessela, C. Mateo, J.M. Palomo, P. Batalla, R. Fernández-Lafuente, and J.M. Guisán Adsorption behavior of bovine serum albumin on lowly activated anionic exchangers suggests a new strategy for solid-phase proteomics Biomacromolecules 7 4 2006 1357 1361 (Pubitemid 43670829)
43. C. Kunz, and B. Lönnerdal Human milk proteins: separation of whey proteins and their analysis by polyacrylamide gel electrophoresis, fast liquid chromatography (FPLC) gel filtration and anion-exchange chromatography Am. J. Clin. Nutr. 49 1989 464 470 (Pubitemid 19107258)
44. P. Mariani, and V. Russo Polmorfismo genetico della -lattalbumina nelle razze bovine Rivista di Zootecnia e Veterinaria 6 1977 603 610
45. K. Baussay, C. Le Bon, T. Nicolai, D. Durand, and J.P. Busnel Influence of the ionic strength on the heat-induced aggregation of the globular protein b-lactoglobulin at pH 7 Int. J. Biol. Macromol. 34 2004 21 28 (Pubitemid 38721181)
46. T.R. Neyestani, M. Djalali, and M. Pezeshki Isolation of a-lactalbumin, b-lactoglobulin, and bovine serum albumin from cow's milk using gel filtration and anion-exchange chromatography including evaluation of their antigenicity Protein Expr. Purif. 29 2003 202 208 (Pubitemid 36720250)
47. M.J. Santos, J.A. Teixeira, and L.R. Rodrigues Fractionation and recovery of whey proteins by hydrophobic interaction chromatography J. Chromatogr. B 879 2011 475 479
48. B. Manji, A. Hill, Y. Kakuda, and D.M. Irvine Rapid separation of milk whey proteins by anion exchange chromatography J. Dairy Sci. 68 1985 3176 3179
49. S. Srikumar, and T.M. Fuchs Ethanolamine utilization contributes to proliferation of Salmonella enterica serovar Typhimurium in food and in nematodes Appl. Environ. Microbiol. 77 1 2011 281 290
50. J.I. Kim, D.Y. Choi, and K.H. Row Separation of whey proteins by anion-exchange membranes Korean J. Chem. Eng. 20 3 2003 538 541 (Pubitemid 36924725)
51. X. Ye, S. Yoshida, and T.B. Ng Isolation of lactoperoxidase, lactoferrin, a-lactalbumin, b-lactoglobulin B and b-lactoglobulin A from bovine rennet whey using ion exchange chromatography Int. J. Biochem. Cell Biol. 32 2000 1143 1150 (Pubitemid 32008302)
52. H. El-Hatmi, J. Girardet, J. Gaillard, T. Khorchani, and H. Attia Therapeutic potential of whey proteins of camel colostrums Microbiologie et Hygine Alimentaire 18 53 2006 70 76