Hyperthermophilic asparaginase mutants with enhanced substrate affinity and antineoplastic activity: Structural insights on their mechanism of action

FASEB Journal

Volumn 26, Issue 3, 2012, Pages 1161-1171

  Bansal, Saurabh ^a   Srivastava, Ankit ^a   Mukherjee, Goutam ^a   Pandey, Ramendra ^b   Verma, Anita Kamra ^b   Mishra, Prashant ^a   Kundu, Bishwajit ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b UNIVERSITY OF DELHI   (India)

Author keywords

Catalytic triad; Leukemia; MD simulation; Pyroccocus furiosus; Stability

Indexed keywords

ANTINEOPLASTIC AGENT; ASPARAGINASE; GLUTAMINASE; PYROCOCCUS FURIOSUS ASPARAGINASE; UNCLASSIFIED DRUG;

ANTINEOPLASTIC ACTIVITY; ARTICLE; CELL STRAIN HL 60; CELL STRAIN MCF 7; CYTOTOXICITY; DRUG STRUCTURE; ENZYME ACTIVITY; ENZYME STABILITY; HUMAN; HUMAN CELL; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS; WILD TYPE;

AMINO ACID SEQUENCE; ANTINEOPLASTIC AGENTS; ARCHAEAL PROTEINS; ASPARAGINASE; CATALYTIC DOMAIN; CELL LINE, TUMOR; CELL SURVIVAL; CIRCULAR DICHROISM; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME STABILITY; HL-60 CELLS; HOT TEMPERATURE; HUMANS; K562 CELLS; KINETICS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; MOLECULAR SEQUENCE DATA; MUTATION; PYROCOCCUS FURIOSUS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; THERMODYNAMICS; TYROSINE;

ESCHERICHIA COLI; PYROCOCCUS FURIOSUS;

EID: 84857749058     PISSN: 08926638     EISSN: 15306860     Source Type: Journal    
DOI: 10.1096/fj.11-191254     Document Type: Article

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