Conformational changes at the active site of bovine pancreatic RNase a at low concentrations of guanidine hydrochloride probed by pyridoxal 5′-phosphate

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1294, Issue 1, 1996, Pages 1-7

  Xiao, Gui Shan ^a   Zhou, Jun Mei ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

Author keywords

(bovine pancreas); Active site; Conformational change; Denaturation; Fluorescence lifetime; Polarization; Pyridoxal 5 phosphate; RNase a

Indexed keywords

BOVINAE;

GUANIDINE; PANCREATIC RIBONUCLEASE;

ARTICLE; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION;

ANIMALS; BINDING SITES; CATTLE; FLUORESCENCE POLARIZATION; GUANIDINE; GUANIDINES; KINETICS; MOLECULAR PROBES; PROTEIN CONFORMATION; PROTEIN DENATURATION; PYRIDOXAL PHOSPHATE; RIBONUCLEASE, PANCREATIC; SPECTROMETRY, FLUORESCENCE; TYROSINE;

EID: 0029979161     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/0167-4838(95)00248-0     Document Type: Article

References (28)

1. Tsou, C.L. (1986) Trends Biochem. Sci. 11, 427-429.
2. Tsou, C.L. (1993) Science 262, 380-381.
3. Ismond, M.A.H., Murray, E.D. and Arntfield, S.D. (1988) Food Chem. 29, 189-198.
4. West, S.M., Kelly, S.M. and Prince, N.C. (1990) Biochim. Biophys. Acta 1037, 332-336.
5. Kelly, S.M. and Prince, N.C. (1991) Biochim. J. 275, 745-749.
6. Nozais, M., Bechet, J.J. and Houadjeto, M. (1992) Biochemistry 31, 1210-1215.
7. Creighton, T.E. (1990) Biochem. J. 270, 1-16.
8. Kelly, S.M. Duncan, D. and Prince N.C. (1993) Int. J. Biol. Macromol. 15, 75-79.
9. Lin, Y.Z., Liang, S.J., Zhou, J.M., Tsou, C.L, Wu, P.Q. and Zhou Z.Q. (1990) Biochim. Biophys Acta 1038, 247-252.
10. Zhang Y.L., Zhou, J.M. and Tsou, C. L.(1993) Biochim. Biophys. Acta 1164, 61-67.
11. Xie, G.F. and Tsou, C.L. (1987) Biochim. Biophys. Acta 911, 19-24.
12. Zhou, H.M., Zhang, X.H., Yin Y. and Tsou, C.L. (1993) Biochem. J. 291, 103-107.
13. Liu, W. and Tsou, C.L., (1987) Biochim. Biophys. Acta 916, 455-464.
14. Anfinsen, C.B, Harrington, W.F., Hvidt, A., Lindstrom-Lang, K. and Ottesen, M. (1955) Biochim. Biophys. Acta 17, 141-142.
15. Raetz, C.R.H. and Auld, D.S. (1972) Biochemistry 11, 2229-2236.
16. Sela, M. and Anfinsen, C.B. (1957) Biochim. Biophys. Acta 24, 229-235.
17. Borisova, S.N., Matrosov, V.J., Shliapnikov, S.V. and Karpeisky, M.Ya. (1974) Mol. Biol. (Moscow) 8, 286-295.
18. Crook, E.M. and Mathias, B.R. (1960) Biochem. J. 74, 234-238.
19. Lakowicz, J.R. (1983) Principles of Fluorescence Spectroscopy, pp. 135-136, Plenum Press, New York.
20. Birch, D.J.S. and Imbof, R.E. (1981) Rev. Sci. Instrum. 52, 1206-1212.
21. Grinvald, A. and Steinberg, I.Z. (1974) Anal. Biochem. 59, 583-593.
22. Durbin, J. and Watson, G.S. (1951) Biometrika 38, 159-178.
23. Lampert, R.A., Chewter, L.A., Phillips, D., O'Conner, D.V., Roberts, A.J. and Meech, S.R. (1983) Anal. Chem. 55, 68-73.
24. Yao, Q.Z., Tian, M and Tsou, C.L. (1984) Biochemistry 23, 2740-2744.
25. Liang, S.J., Lin, Y.Z., Zhou, J.M., Tsou, C.L., Wu, P.Q. and Zhou, Z.K. (1990) Biochim. Biophys. Acta 1038, 243-246.
26. Ma, Y.Z. and Tsou, C.L.,(1995) Biochem. J. 277, 207-211.
27. Yang, H.J. and Tsou, C.L. (1995) Biochem. J. 305, 379-384.
28. Yao, Q.Z., Zhou, J.M., Hou, L.X. and Tsou, C.L., (1982) Sci. Sin. 26b, 1296-1302.