Dynamic mechanism of proton transfer in mannitol 2-dehydrogenase from Pseudomonas fluorescens: Mobile glu 292 controls proton relay through a water channel that connects the active site with bulk solvent

Journal of Biological Chemistry

Volumn 287, Issue 9, 2012, Pages 6655-6667

  Klimacek, Mario ^a   Brunsteiner, Michael ^a,b   Nidetzky, Bernd ^a,b  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b RESEARCH CENTER PHARMACEUTICAL ENGINEERING GMBH   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ALCOHOL DEHYDROGENASE; BULK SOLVENTS; CATALYTIC ACIDS; CHANNEL OPENING; CHARGE STATE; CONFORMATIONAL CHANGE; CONFORMATIONAL DYNAMICS; DYNAMIC MECHANISM; ENZYMATIC CATALYSIS; KEY ELEMENTS; MOLECULAR DYNAMICS SIMULATIONS; PHYSIOLOGICAL PH; PROTEIN CHANNELS; PROTON CHANNELS; PROTON PUMP; PROTON TRANSLOCATION; PSEUDOMONAS FLUORESCENS; REACTION PROGRESS; RELAY FUNCTIONS; SITE DIRECTED MUTAGENESIS; WATER CHAINS; WATER CHANNELS; WATER MOLECULE;

BACTERIA; CATALYSIS; CATALYTIC OXIDATION; MOLECULAR DYNAMICS; PH; PROTON TRANSFER; SOLVENTS;

DRUG PRODUCTS;

ALANINE; ALCOHOL DEHYDROGENASE; GLUTAMIC ACID; LYSINE; MANNITOL; MANNITOL DEHYDROGENASE; MUTANT PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; PROTON; SOLVENT; WATER;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CHANNEL GATING; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME CONFORMATION; MOLECULAR DYNAMICS; NONHUMAN; OXIDATION; PH; PRIORITY JOURNAL; PROTEIN TRANSPORT; PROTON TRANSPORT; PSEUDOMONAS FLUORESCENS; SIMULATION; SITE DIRECTED MUTAGENESIS;

BACTERIAL PROTEINS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY; ENZYME ACTIVATION; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; MANNITOL DEHYDROGENASES; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; PROTONS; PSEUDOMONAS FLUORESCENS; WATER;

PSEUDOMONAS FLUORESCENS; PYRONIA TITHONUS;

EID: 84857499524     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.289223     Document Type: Article

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