L-Aspartate dehydrogenase: Features and applications

Applied Microbiology and Biotechnology

Volumn 93, Issue 2, 2012, Pages 503-516

  Li, Yinxia ^a   Ogola, Henry Joseph Oduor ^a,b   Sawa, Yoshihiro ^a  

^a SHIMANE UNIVERSITY   (Japan)

^b Bondo University College   (Kenya)

Author keywords

Applications; Characteristics; Functions; l Amino acid dehydrogenase; l Aspartate dehydrogenase; NAD biosynthesis

Indexed keywords

BIOTECHNOLOGICAL APPLICATIONS; CATALYTIC PROPERTIES; CHARACTERISTICS; COENZYME REGENERATION SYSTEMS; COFACTORS; DIAGNOSTIC KIT; IN-VIVO; INDUSTRIAL PROCESSS; L-AMINO ACID DEHYDROGENASE; L-AMINO ACIDS; L-ASPARTATE DEHYDROGENASE; MESOPHILIC; NEW MEMBERS; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATES; NICOTINAMIDE ADENINE DINUCLEOTIDES; OXIDATIVE DEAMINATION; PHARMACEUTICAL PEPTIDES; POTENTIAL APPLICATIONS; ROOM TEMPERATURE;

AMIDES; APPLICATIONS; BIOCHEMISTRY; BIOINFORMATICS; BIOSENSORS; BIOSYNTHESIS; ENZYMES; FUNCTIONS; HERBICIDES; NUCLEOTIDES; POLYPEPTIDES;

AMINO ACIDS;

ALANINE DEHYDROGENASE; ASPARTATE DEHYDROGENASE; GLUTAMATE DEHYDROGENASE; LEUCINE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; PHENYLALANINE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UNCLASSIFIED DRUG; VALINE DEHYDROGENASE;

AMINO ACID; BIOTECHNOLOGY; CATALYSIS; ENZYME ACTIVITY; INDUSTRIAL PRODUCTION; OXIDATION; TEMPERATURE EFFECT;

AMINO ACID SEQUENCE; BIOCATALYST; CATALYSIS; DIET SUPPLEMENTATION; ENZYME STABILITY; IN VIVO STUDY; MOLECULAR WEIGHT; NONHUMAN; PH; PHYLOGENY; REVIEW; ROOM TEMPERATURE; THERMOSTABILITY;

AMINO ACID OXIDOREDUCTASES; ASPARTIC ACID; BIOTECHNOLOGY; NAD; OXIDATION-REDUCTION;

EID: 84856300030     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3730-4     Document Type: Review

References (103)

1. Ansorge MB, Kula MR (2000) Production of recombinant L-leucine dehydrogenase from Bacillus cereus in pilot scale using the runaway replication system E. coli [pIET98]. Biotechnol Bioeng 68:557-562 (Pubitemid 30307726)
2. Asano Y, Tanetani M (1998) Thermostable phenylalanine dehydrogenase from a mesophilic Microbacterium sp. strain DM 86-1. Arch Microbiol 169:220-224 (Pubitemid 28098693)
3. Asano Y, Nakazawa A, Endo K (1987a) Novel phenylalanine dehydrogenases from Sporosarcina ureae and Bacillus sphaericus. Purification and characterization. J Biol Chem 262:10346-10354
4. Asano Y, Nakazawa A, Endo K, Hibino Y, Ohmori M, Numao N, Kondo K (1987b) Phenylalanine dehydrogenase of Bacillus badius. Purification, characterization and gene cloning. Eur J Biochem 168:153-159
5. Baars JJ, Op den Camp HJ, van Hoek AH, van der Drift C, Van Griensven LJ, Visser J, Vogels GD (1995) Purification and characterization of NADP-dependent glutamate dehydrogenase from the commercial mushroom Agaricus bisporus. Curr Microbiol 30:211-217
6. Baker PJ, Turnbull AP, Sedelnikova SE, Stillman TJ, Rice DW (1995) A role for quaternary structure in the substrate specificity of leucine dehydrogenase. Structure 3:693-705
7. Baker PJ, Sawa Y, Shibata H, Sedelnikova SE, Rice DW (1998) Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase. Nat Struct Biol 5:561-567
8. Barker HA (1981) Amino acid degradation by anaerobic bacteria. Annu Rev Biochem 50:23-40
9. Begley TP, Kinsland C, Mehl RA, Osterman A, Dorrestein P (2001) The biosynthesis of nicotinamide adenine dinucleotides in bacteria. Vitam Horm 61:103-119 (Pubitemid 33805692)
10. Bergmeyer HU, Bernt E, Moellering H, Pfleiderer G (1974) L-Aspartate and L-asparagine. In: Bergmeyer HU (ed) Methods of enzymatic analysis, 2nd edn. Academic, New York, pp 1696-1700
11. Bhuiya MW, Sakuraba H, Ohshima T, Imagawa T, Katunuma N, Tsuge H (2005) The first crystal structure of hyperthermostable NAD-dependent glutamate dehydrogenase from Pyrobaculum islandicum. J Mol Biol 345:325-337 (Pubitemid 39574854)
12. Bommarius AS, Schwarm M, Drauz K (1998) Biocatalysis to amino acid-based chiral pharmaceuticals-examples and perspectives. J Mol Catal B: Enzym 5:1-11 (Pubitemid 28429167)
13. Britton KL, Baker PJ, Engel PC, Rice DW, Stillman TJ (1993) Evolution of substrate diversity in the superfamily of amino acid dehydrogenases. Prospects for rational chiral synthesis. J Mol Biol 234:938-945
14. Britton KL, Yip KS, Sedelnikova SE, Stillman TJ, Adams MW, Ma K, Maeder DL, Robb FT, Tolliday N, Vetriani C, Rice DW, Baker PJ (1999) Structure determination of the glutamate dehydrogenase from the hyperthermophile Thermococcus litoralis and its comparison with that from Pyrococcus furiosus. J Mol Biol 293:1121-1132
15. Brunhuber NM, Blanchard JS (1994) The biochemistry and enzymology of amino acid dehydrogenases. Crit Rev Biochem Mol Biol 29:415-467
16. Busca P, Paradisi F, Moynihan E, Maguirem AR, Engel PC (2004) Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis. Org Biomol Chem 2:2684-2691
17. Cainelli G, Engel PC, Galletti P, Giacomini D, Gualandi A, Paradisi F (2005) Engineered phenylalanine dehydrogenase in organic solvents: homogeneous and biphasic enzymatic reactions. Org Biomol Chem 3:4316-4320 (Pubitemid 43035614)
18. Chao Y, Lo T, Luo N (2000) Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli. Enzyme Microb Technol 27:19-25 (Pubitemid 30345482)
19. Chen S, Engel PC (2007) An engineered mutant, L307V of phenylalanine dehydrogenase from Bacillus sphaericus: high activity and stability in organic-aqueous solvent mixtures and utility for synthesis of non-natural l-amino acids. Enzyme Microb Technol 40:1407-1411 (Pubitemid 46356553)
20. Crow VL (1987) Properties of alanine dehydrogenase and aspartase from Propionibacterium freudenreichii subsp. shermanii. Appl Environ Microbiol 53:1885-1892
21. +-dependent glutamate dehydrogenase in the Antarctic psychrotolerant bacterium Psychrobacter sp. TAD1. Characterization, protein and DNA sequence, and relationship to other glutamate dehydrogenases. Eur J Biochem 267:121-131 (Pubitemid 30029745)
22. Diruggiero J, Robb FT (1995) Expression and in vitro assembly of recombinant glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus. Appl Environ Microbiol 61:159-164
23. Dooley KC (1992) Enzymatic method for phenylketonuria screening using phenylalanine dehydrogenase. Clin Biochem 25:271-275
24. Esaki N, Shimoi H, Nakajima N, Ohshima T, Tanaka H, Soda K (1989) Enzymatic in situ determination of stereospecificity of NAD-dependent dehydrogenases. J Biol Chem 264:9750-9752 (Pubitemid 19159235)
25. Esaki N, Nakajima N, Nakamura K, Yonaha K, Tanaka H, Soda K (1990) Preparation of stereoselectively-deuterated NADH and NADPH by coupling of glutamate racemase and glutamate dehydrogenase. Biotechnol Lett 12:105-110 (Pubitemid 20116576)
26. Fukuoka S, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada H, Shibata K (2002) Identification and expression of a cDNA encoding human alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD). A key enzyme for the tryptophan-niacine pathway and "quinolinate hypothesis". J Biol Chem 277:35162-35167
27. Hanson RL, Singh J, Kissick TP, Patel RN, Szarka LJ, Mueller RH (1990) Synthesis of L-hydrohyvaline from keto hydroxyisovalerate using LeuDH from Bacillus species. Bioorg Chem 18:116-130 (Pubitemid 20285506)
28. Hornby DP, Engel PC (1984) Characterization of Peptostreptococcus asaccharolyticus glutamate dehydrogenase purified by dye-ligand chromatography. J Gen Microbiol 130:2385-2394 (Pubitemid 14007215)
29. Hudson RC, Daniel RM (1993) L-Glutamate dehydrogenases: distribution, properties and mechanism. Comp Biochem Physiol 106:767-792
30. Hummel W, Weiss N, Kula MR (1984) Isolation and characterization of a bacterium possessing L-phenylalanine dehydrogenase activity. Arch Microbiol 137:47-52 (Pubitemid 14191495)
31. Hummel W, Schuette H, Schmidt E, Wandrey C, Kula MR (1987) Isolation of L-phenylalanine dehydrogenase from Rhodococcus sp. M4 and its application for the production of L-phenylalanine. Appl Microbiol Biotechnol 26:409-416
32. Hummel W, Kuzu M, Geueke B (2003) An efficient and selective enzymatic oxidation system for the synthesis of enantiomerically pure D-tert-leucine. Org Lett 5:3649-3650 (Pubitemid 37289546)
33. Hyun CG, Kim SS, Park KH, Suh JW (2000) Valine dehydrogenase from Streptomyces albus: gene cloning, heterologous expression and identification of active site by site-directed mutagenesis. FEMS Microbiol Lett 182:29-34 (Pubitemid 30001560)
34. Irwin JA, Lynch SV, Coughlan S, Baker PJ, Gudmundsson HM, Alfredsson GA, Rice DW, Engel PC (2003) Alanine dehydrogenase from the psychrophilic bacterium strain PA-43: overexpression, molecular characterization, and sequence analysis. Extremophiles 7:135-143 (Pubitemid 40924483)
35. Johnson WM, Westlake DWS (1972) Purification and characterization of glutamic acid dehydrogenase and alphaketoglutaric acid reductase from Peptococcus aerogenes. Can J Microbiol 18:881-892
36. Kagan ZS, Poliakov VA, Kretovich VL (1968) Soluble valine dehydrogeaase from roots of plant seedlings. Biokhimiia 33:89-96
37. Kagan ZS, Poliakov VA, Kretovich VL (1969) Purification and properties of valine dehydrogenase. Biokhimiia 34:59-65
38. Kamphuis J, Meijer E, Boesten W, Broxterman Q, Kaptein B, Hermes H, Schoemaker H (1992) Production of natural and synthetic L- and D-amino acids by aminopeptidases and amino amidases. In: Rozzell D, Wagner F (eds) Biocatalytic production of amino acids and derivatives. Hanser, Munich, pp 177-206
39. Kataoka K, Tanizawa K (2003) Alteration of substrate specificity of leucine dehydrogenase by site-directed mutagenesis. J Mol Catal B: Enzym 23:299-309 (Pubitemid 37011205)
40. Katoh A, Hashimoto T (2004) Molecular biology of pyridine nucleotide and nicotine biosynthesis. Front Biosci 9:1577-1586 (Pubitemid 38918616)
41. Katoh R, Ngata S, Ozawa A, Ohshima T, Kamekura M, Misono H (2003) Purification and characterization of leucine dehydrogenase from an alkalophilic halophile, Natronobacterium magadii MS-3. J Mol Catal B: Enzym 23:231-238 (Pubitemid 37011197)
42. Khan IH, Ito K, Kim H, Ashida H, Ishikawa T, Shibata H, Sawa Y (2005a) Molecular properties and enhancement of thermostability by random mutagenesis of glutamate dehydrogenase from Bacillus subtilis. Biosci Biotechnol Biochem 69:1861-1870 (Pubitemid 41558849)
43. Khan IH, Kim H, Ashida H, Ishikawa T, Shibata H, Sawa Y (2005b) Altering the substrate specificity of glutamate dehydrogenase from Bacillus subtilis by site-directed mutagenesis. Biosci Biotechnol Biochem 69:1802-1805 (Pubitemid 41390176)
44. Knapp S, de Vos WM, Rice D, Ladenstein R (1997) Crystal structure of glutamate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima at 3.0A resolution. J Mol Biol 267:916-932 (Pubitemid 27192663)
45. Kragl U, Vasic-Racki D, Wandrey C (1996) Continuous production of L-tert-leucine in series of two enzyme membrane reactors-modelling and computer simulation. Bioprocess Eng 14:291-297
46. Krixa G, Bommariusb AS, Drauzb K, Kottenhahnb M, Schwarmb M, Kulaa MR (1997) Enzymatic reduction of α-keto acids leading to l-amino acids, d- or l-hydroxy acids. J Biotechnol 53:29-39
47. Lee KH, Huh JW, Choi MM, Yoon SY, Yang SJ, Hong HN, Cho SW (2005) Regulation of glutamate level in rat brain through activation of glutamate dehydrogenase by Corydalis ternata. Exp Mol Med 37:371-377 (Pubitemid 41330047)
48. Li H, Zhu D, Hyatt BA, Malik FM, Biehl ER, Hua L (2008) Cloning, protein sequence clarification, and substrate specificity of a leucine dehydrogenase from Bacillus sphaericus ATCC4525. Appl Biochem Biotechnol 158:343-351
49. Li Y, Ishida M, Ashida H, Ishikawa T, Shibata H, Sawa Y (2011a) A non-NadB type L-aspartate dehydrogenase from Ralstonia eutropha strain JMP134: molecular characterization and physiological functions. Biosci Biotechnol Biochem 75:1524-1532
50. Li Y, Kawakami N, Ogola HJO, Ashida H, Ishikawa T, Shibata H, Sawa Y (2011b) A novel L-aspartate dehydrogenase from the mesophilic bacterium Pseudomonas aeruginosa PAO1: molecular characterization and application for L-aspartate production. Appl Microbiol Biotechnol 90:1953-1962
51. Livesey G, Lund P (1988) Determination of branched-chain amino and keto acids with leucine dehydrogenase. Methods Enzymol 166:3-10 (Pubitemid 19048571)
52. Marinoni I, Nonnis S, Monteferrante C, Heathcote P, Härtig E, Böttger LH, Trautwein AX, Negri A, Albertini AM, Tedeschi G (2008) Characterization of L-aspartate oxidase and quinolinate synthase from Bacillus subtilis. FEBS J 275:5090-5107
53. Massey LK, Sokatch JR, Conrad RS (1976) Branched-chain amino acid catabolism in bacteria. Bacteriol Rev 40:42-54
54. Mattevi A (2006) A close look at NAD biosynthesis. Nat Struct Mol Biol 13:563-564 (Pubitemid 44036461)
55. Mattevi A, Tedeschi G, Bacchella L, Coda A, Negri A, Ronchi S (1999) Structure of L-aspartate oxidase: implications for the succinate dehydrogenase/fumarate reductase oxidoreductase family. Structure 7:745-756 (Pubitemid 29329846)
56. McClelland M, Sanderson EK, Spieth J, Clifton WS, Latreille P, Sabo A, Pepin K, Bhonagiri V, Porwollik S, Ali J, Wilson RK (2006) Submitted to the EMBL/GenBank/DDBJ databases
57. Merrick MJ, Edwards RA (1995) Nitrogen control in bacteria. Microbiol Rev 59:604-622
58. Murachi T, Tabata M (1987) Use of bio-reactor consisting of sequentially aligned L-glutamate dehydrogenase and L-glutamate oxidase for the determination of ammonia by chemiluminescence. Biotechnol Appl Biochem 9:303-309
59. Muranova TA, Ruzheinikov SN, Sedelnikova SE, Baker PJ, Pasquo A, Galkin A, Esaki N, Ohshima T, Soda K, Rice DW (2002) Crystallization and preliminary X-ray analysis of substrate complexes of leucine dehydrogenase from Thermoactinomyces intermedius. Acta Crystallogr D Biol Crystallogr 58:1059-1062 (Pubitemid 34653044)
60. Mutaguchi Y, Ohmori T, Sakuraba H, Yoneda K, Doi K, Ohshima T (2011) Visible wavelength spectrophotometric assays of L-aspartate and D-aspartate using hyperthermophilic enzyme systems. Anal Biochem 409:1-6
61. +-dependent dehydrogenases. Biochemistry 27(9056):9062
62. Nagata S, Bakthavatsalam S, Galkin AG, Asada H, Sakai S, Esaki N, Soda K, Ohshima T, Nagasaki S, Misono H (1995) Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9. Appl Microbiol Biotechnol 44:432-438 (Pubitemid 26024362)
63. Nasu S, Wicks FD, Gholson RK (1982) L-Aspartate oxidase, a newly discovered enzyme of Escherichia coli, is the B protein of quinolinate synthetase. J Biol Chem 257:626-632 (Pubitemid 12110193)
64. Navarrete RM, Vara JA, Hutchinson CR (1990) Purification of an inducible L-valine dehydrogenase of Streptomyces coelicolor A3(2). J Gen Microbiol 136:273-281 (Pubitemid 20059108)
65. Ohshima T, Soda K (1989) Thermostable amino acid dehydrogenases: applications and gene cloning. Trends Biotechnol 7:210-214 (Pubitemid 19189285)
66. Ohshima T, Soda K (1990) Biochemistry and biotechnology of amino acid dehydrogenases. Adv Biochem Eng Biotechnol 42:187-209
67. Ohshima T, Soda K (2000) Stereoselective biocatalysis: amino acid dehydrogenases and their applications. In: Patel RN (ed) Stereoselective biocatalysis. Marcel Dekker, New York, USA, pp 877-903
68. Ohshima T, Yamamoto T, Misono H, Soda K (1978) Leucine dehydrogenase of Bacillus sphaericus: sulfhydryl groups and catalytic sites. Agric Biol Chem 42:1739-1743 (Pubitemid 9039425)
69. Ohshima T, Nishida N, Bakthavatsalam S, Kataoka K, Takada H, Yoshimura T, Esaki N, Soda K (1994) The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius. Eur J Biochem 222:305-312 (Pubitemid 24204899)
70. Oikawa T, Yamanaka K, Kazuoka T, Kanzawa N, Soda K (2001) Psychrophilic valine dehydrogenase of the antarctic psychrophile, Cytophaga sp. KUC-1: purification, molecular characterization and expression. Eur J Biochem 268:4375-4383 (Pubitemid 32862846)
71. Okamura T, Noda H, Fukuda S, Ohsugi M (1998) Aspartate dehydrogenase in vitamin B12-producing Klebsiella pneumoniae IFO 13541. J Nutr Sci Vitaminol 44:483-490 (Pubitemid 28456022)
72. Omidinia E, Samadi A, Taherkhani H, Khatami S, Moazami N, Pouraie RR, Asano Y (2002) Cloning and expression of Bacillus sphaericus phenylalanine dehydrogenase gene in Bacillus subtilis cells: purification and enzyme properties. World J Microbiol Biotechnol 18:593-597
73. Paradisi F, Collins S, Maguire AR, Engel PC (2007) Phenylalanine dehydrogenase mutants: efficient biocatalysts for synthesis of non-natural phenylalanine derivatives. J Biotechnol 128:408-411 (Pubitemid 46074186)
74. Peterson PE, Smith TJ (1999) The structure of bovine glutamate dehydrogenase provides insights into the mechanism of allostery. Structure Fold Des 7:769-782 (Pubitemid 29329848)
75. Priestley ND, Robinson JA (1989) Purification and catalytic properties of L-valine dehydrogenase from Streptomyces cinnamonensis. Biochem J 261:853-861 (Pubitemid 19209526)
76. Qiu X, Xie W, Lian X, Zhang Q (2009) Molecular analyses of the rice glutamate dehydrogenase gene family and their response to nitrogen and phosphorous deprivation. Plant Cell Rep 28:1115-1126
77. Sanwal BD, Zink MW (1961) L-Leucine dehydrogenase of Bacillus cereus. Arch Biochem Biophys 94:430-435
78. Sawa Y, Tani M, Murata K, Shibata H, Ochiai H (1994) Purification and characterization of alanine dehydrogenase from a cyanobacterium, Phormidium lapideum. J Biochem 116:995-1000 (Pubitemid 24354647)
79. Schröder I, Vadas A, Johnson E, Lim S, Monbouquette HG (2004) A novel archaeal alanine dehydrogenase homologous to ornithine cyclodeaminase and mu-crystallin. J Bacteriol 186:7680-7689 (Pubitemid 39463734)
80. Seah SYK (2007) Amino acid dehydrogenases. In: Polaina J, MacCabe AP (eds) Industrial enzymes: structure, function, and applications. Springer, Dordrecht, pp 489-504
81. Seah SYK, Britton KL, Baker PJ, Rice DW, Asano Y, Engel PC (1995) Alteration in relative activities of phenylalanine dehydrogenase towards different substrates by site-directed mutagenesis. FEBS Lett 370:93-96
82. Seah SYK, Britton KL, Rice DW, Asano Y, Engel PC (2002) Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates. Biochemistry 41:11390-11397
83. Shimoi H, Nagata S, Esaki N, Tanaka H, Soda K (1987) Leucine dehydrogenase of a thermophilic anaerobe, Clostridium thermoaceticum: gene cloning, purification and characterization. Agric Biol Chem 51:3375-3381
84. Smith MT, Emerich DW (1993) Alanine dehydrogenase from soybean nodule bacteroids: purification and properties. Arch Biochem Biophys 304:379-385 (Pubitemid 23232951)
85. Smith TJ, Schmidt T, Fang J, Wu J, Siuzdak G, Stanley CA (2002) The structure of apo human glutamate dehydrogenase details subunit communication and allostery. J Mol Biol 318:765-777 (Pubitemid 34729367)
86. Stillman TJ, Baker PJ, Britton KL, Rice DW (1993) Conformational flexibility in glutamate dehydrogenase. Role of water in substrate recognition and catalysis. J Mol Biol 234:1131-1139
87. Strecker HJ (1951) Crystallization of L-glutamic acid dehydrogenase from liver. Arch Biochem Biophys 32:448-449
88. Takada H, Yoshimura T, Ohshima T, Esaki N, Soda K (1991) Thermostable phenylalanine dehydrogenase of Thermoactinomyces intermedius: cloning, expression, and sequencing of its gene. J Biochem 109:371-376
89. Tamura K, Peterson D, Stecher G, Nei M, Kumar S (2011) MEGA: Molecular evolutionary genetic analysis using maximum likelihood, evolutionary distance and maximum parsimony method. Mol Biol Evol. doi: 10.1093/molbev/msr121
90. Tritz GJ (1987) NAD biosynthesis and recycling. In: Neidhart FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) In Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, pp 557-563
91. Turnbull AP, Baker PJ, Rice DW (1997) Analysis of the quaternary structure, substrate specificity, and catalytic mechanism of valine dehydrogenase. J Biol Chem 272:25105-25111 (Pubitemid 27415693)
92. Vancura A, Vancurová I, Volc J, Fussey SP, Flieger M, Neuzil J, Marsálek J, Běhal V (1988) Valine dehydrogenase from Streptomyces fradiae: purification and properties. J Gen Microbiol 134:3213-3219
93. Vancurová I, Vancura A, Volc J, Neuzil J, Flieger M, Basarová G, Běhal V (1988a) Isolation and characterization of valine dehydrogenase from Streptomyces aureofaciens. J Bacteriol 170:5192-5196 (Pubitemid 18259461)
94. Vancurová I, Vancura A, Volc J, Neuzil J, Flieger M, Basarová G, Běhal V (1988b) Purification and partial characterization of alanine dehydrogenase from Streptomyces aureofaciens. Arch Microbiol 150:438-440
95. Vanhooke JL, Thoden JB, Brunhuber NM, Blanchard JS, Holden HM (1999) Phenylalanine dehydrogenase from Rhodococcus sp. M4: high-resolution X-ray analyses of inhibitory ternary complexes reveal key features in the oxidative deamination mechanism. Biochemistry 38:2326-2339
96. Villalonga R, Tachibana S, Perez Y, Asano Y (2005) Increased conformational and thermal stability properties for phenylalanine dehydrogenase by chemical glycosidation with end-group activated dextran. Biotechnol Lett 27:1311-1317 (Pubitemid 41428182)
97. Wakayama M, Takashima K, Tau Y, Nakashima S, Sakai K, Moriguchi M (1997) Spectrophotometric assay of D-aspartate and D-glutamate using D-aspartate oxidase with malate dehydrogenase and glutamate dehydrogenase. Anal Biochem 250:252-253 (Pubitemid 27319104)
98. Werner C, Stubbs MT, Krauth-Siegel RL, Klebe G (2005) The crystal structure of Plasmodium falciparum glutamate dehydrogenase, a putative target for novel antimalarial drugs. J Mol Biol 349:597-607 (Pubitemid 40724568)
99. Yamaguchi A, Mizushima Y, Fukushi M, Shimizu Y, Kikuchi Y, Takasugi N (1992) Microassay system for newborn screening for phenylketonuria, maple syrup urine disease, homocystinuria, histidinemia and galactosemia with use of a fluorometric microplate reader. Screening 1:49-62
100. Yang Z, Savchenko A, Yakunin A, Zhang R, Edwards A, Arrowsmith C, Tong L (2003) Aspartate dehydrogenase, a novel enzyme identified from structural and functional studies of TM1643. J Biol Chem 278:8804-8808 (Pubitemid 36800638)
101. Yip KS, Stillman TJ, Britton KL, Artymiuk PJ, Baker PJ, Sedelnikova SE, Engel PC, Pasquo A, Chiaraluce R, Consalvi V (1995) The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures. Structure 3:1147-1158 (Pubitemid 3001432)
102. Yoneda K, Kawakami R, Tagashira Y, Sakuraba H, Goda S, Ohshima T (2006) The first archaeal L-aspartate dehydrogenase from the hyperthermophile Archaeoglobus fulgidus: gene cloning and enzymological characterization. Biochim Biophys Acta 1764:1087-1093
103. Yoneda K, Sakuraba H, Tsuge H, Katunuma N, Ohshima T (2007) Crystal structure of archaeal highly thermostable L-aspartate dehydrogenase/NAD/citrate ternary complex. FEBS J 274:4315-4325 (Pubitemid 47228650)