Cloning, protein sequence clarification, and substrate specificity of a leucine dehydrogenase from bacillus sphaericus ATCC4525

Applied Biochemistry and Biotechnology

Volumn 158, Issue 2, 2009, Pages 343-351

  Li, Hongmei ^a   Zhu, Dunming ^a,b   Hyatt, Brooke A ^a   Malik, Fahad M ^a   Biehl, Edward R ^a   Hua, Ling ^a,c  

^a SOUTHERN METHODIST UNIVERSITY   (United States)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c China Research Center   (China)

Author keywords

Bacillus sphaericus; Gene cloning; Leucine dehydrogenase; Reductive amination; Synthesis of amino acids

Indexed keywords

AMINO ACID RESIDUES; AMINO ACID SEQUENCE; ASYMMETRIC SYNTHESIS; BACILLUS SPHAERICUS; CATALYTIC PROPERTIES; GENE CLONING; HIS-TAGGED PROTEINS; KETOACIDS; LEUCINE DEHYDROGENASE; OXIDATIVE DEAMINATION; PROTEIN SEQUENCES; RECOMBINANT ENZYMES; REDUCTIVE AMINATION; SUBSTRATE SPECIFICITY; X-RAY MODELS;

AMINATION; AMINES; BACTERIOLOGY; CLONING; ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; GENE ENCODING; ORGANIC ACIDS; SUBSTRATES;

AMINO ACIDS;

2 OXOACID; ALPHA AMINO ACID; HISTONE; LEUCINE; LEUCINE DEHYDROGENASE; AMINO ACID; RECOMBINANT PROTEIN;

AMINATION; AMINO ACID SEQUENCE; ARTICLE; ASYMMETRIC SYNTHESIS; BACILLUS SPHAERICUS; CATALYST; CLONING; DEAMINATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; NONHUMAN; BACILLUS; CATALYSIS; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY;

BACILLUS SPHAERICUS; ESCHERICHIA COLI;

AMINO ACID SEQUENCE; AMINO ACIDS; BACILLUS; CATALYSIS; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; KINETICS; LEUCINE DEHYDROGENASE; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 70349320054     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-008-8304-2     Document Type: Article

References (9)

1. K. Kataoka K. Tanizawa 2003 Alteration of substrate specificity of leucine dehydrogenase by site-directed mutagenesis Journal of Molecular Catalysis. B, Enzymatic 23 299 309 10.1016/S1381-1177(03)00093-6 1:CAS:528:DC%2BD3sXms12itL4%3D doi:10.1016/S1381-1177(03)00093-6 (Pubitemid 37011205)
2. P. J. Baker A. P. Turnbull S. E. Sedelnikova T. J. Stillman D. W. Rice 1995 A role for quaternary structure in the substrate specificity of leucine dehydrogenase Structure (London) 3 693 705 1:CAS:528:DyaK2MXntF2rsr0%3D
3. +-dependent dehydrogenases Biochemistry 27 9056 9062 10.1021/bi00425a026 1:CAS:528:DyaL1MXhvVWi doi:10.1021/bi00425a026
4. T. Ohshima N. Nishida S. Bakthavatsalam K. Kataoka H. Takada T. Yoshimura, et al. 1994 The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius European Journal of Biochemistry 222 305 312 10.1111/j.1432-1033.1994.tb18869.x 1:CAS:528:DyaK2cXlt1Kmtb0%3D doi:10.1111/j.1432-1033.1994.tb18869.x (Pubitemid 24204899)
5. R. Katoh S. Nagata H. Misono 2003 Cloning and sequencing of the leucine dehydrogenase gene from Bacillus sphaericus IFO 3525 and importance of the C-terminal region for the enzyme activity Journal of Molecular Catalysis. B, Enzymatic 23 239 247 10.1016/S1381-1177(03)00086-9 1:CAS:528: DC%2BD3sXms12it70%3D doi:10.1016/S1381-1177(03)00086-9 (Pubitemid 37011198)
6. H. Lineweaver D. Burk 1934 Determination of enzyme dissociation constants Journal of the American Chemical Society 56 658 666 10.1021/ja01318a036 1:CAS:528:DyaA2cXisF2ltg%3D%3D doi:10.1021/ja01318a036
7. +-dependent leucine dehydrogenase from Bacillus sphaericus Journal of Molecular Biology 236 663 665 10.1006/jmbi.1994.1176 1:CAS:528: DyaK2cXitl2jur0%3D doi:10.1006/jmbi.1994.1176 (Pubitemid 24146310)
8. T. Ohshima H. Misono K. Soda 1978 Properties of crystalline leucine dehydrogenase from Bacillus sphaericus The Journal of Biological Chemistry 253 5719 5725 1:CAS:528:DyaE1cXlsF2hu7k%3D (Pubitemid 8403706)
9. R. Katoh S. Ngata A. Ozawa T. Ohshima M. Kamekura H. Misono 2003 Purification and characterization of leucine dehydrogenase from an alkalophilic halophile, Natronobacterium magadii MS-3 Journal of Molecular Catalysis. B, Enzymatic 23 231 238 10.1016/S1381-1177(03)00085-7 1:CAS:528: DC%2BD3sXms12it7w%3D doi:10.1016/S1381-1177(03)00085-7 (Pubitemid 37011197)