Valine dehydrogenase from Streptomyces albus: Gene cloning, heterologous expression and identification of active site by site-directed mutagenesis

FEMS Microbiology Letters

Volumn 182, Issue 1, 2000, Pages 29-34

  Hyun, Chang Gu ^a   Kim, Sang Suk ^a   Park, Kwan Hyung ^a   Suh, Joo Won ^a  

^a Myong Ji University   (South Korea)

Author keywords

Active site; Cloning; Site directed mutagenesis; Streptomyces albus; Valine dehydrogenase

Indexed keywords

2 AMINOBUTYRIC ACID; OXIDOREDUCTASE; VALINE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENE OVEREXPRESSION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; STEADY STATE; STREPTOMYCES ALBUS;

AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; BINDING SITES; CLONING, MOLECULAR; ESCHERICHIA COLI; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE ANALYSIS, DNA; STREPTOMYCES; SUBSTRATE SPECIFICITY; VALINE; VALINE DEHYDROGENASE (NADP+);

ESCHERICHIA COLI; STREPTOMYCES ALBUS;

EID: 0033988539     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00561-3     Document Type: Article

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