Phenylalanine dehydrogenase mutants: Efficient biocatalysts for synthesis of non-natural phenylalanine derivatives

Journal of Biotechnology

Volumn 128, Issue 2, 2007, Pages 408-411

  Paradisi, Francesca ^a   Collins, Stuart ^b   Maguire, Anita R ^b   Engel, Paul C ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

Biocatalysis; Chiral synthesis; Cofactor recycling; Non natural amino acids; Phenylalanine dehydrogenase; Site directed mutagenesis

Indexed keywords

AMINO ACIDS; BIOCATALYSTS; BIOSYNTHESIS; DERIVATIVES; PARAMETER ESTIMATION; PROTEINS;

CHIRAL SYNTHESIS; COFACTOR RECYCLING; NONNATURAL AMINO ACIDS; PHENYLALANINE DEHYDROGENASE; SITE DIRECTED MUTAGENESIS;

ENZYME KINETICS;

AMINO ACID DERIVATIVE; OXIDOREDUCTASE; PHENYLALANINE DERIVATIVE; PYRUVIC ACID DERIVATIVE;

ARTICLE; BACILLUS SPHAERICUS; BIOCATALYST; COMPARATIVE STUDY; KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; SYNTHESIS; WILD TYPE;

AMINO ACID OXIDOREDUCTASES; BIOTRANSFORMATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; STEREOISOMERISM;

BACILLUS SPHAERICUS;

EID: 33846105845     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.08.008     Document Type: Article

References (6)

1. Asano Y., Yamada A., Kato Y., Yamaguchi K., Hibino Y., Hirai K., and Kondo K. Enantioselective synthesis of (S)-amino acids by phenylalanine dehydrogenase from Bacillus sphaericus: use of natural and recombinant enzymes. J. Org. Chem. 55 (1990) 5567-5571
2. Busca P., Paradisi F., Moynihan E., Maguire A.R., and Engel P.C. Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis. Org. Biomol. Chem. 2 (2004) 2684-2691
3. Cainelli G., Engel P.C., Galletti P., Giacomini D., Gualandi A., and Paradisi F. Engineered phenylalanine dehydrogenase in organic solvents: homogeneous and biphasic enzymatic reactions. Org. Biomol. Chem. 3 (2005) 4316-4320
4. Meiwes J., Schudok M., and Kretzschmar G. Asymmetric synthesis of l-thienylalanines. Tetrahedron: Asymmetry 8 (1997) 527-536
5. Seah S.Y., Britton K.L., Baker P.J., Rice D.W., Asano Y., and Engel P.C. Alteration in relative activities of phenylalanine dehydrogenase towards different substrates by site-directed mutagenesis. FEBS Lett. 370 (1995) 93-96
6. Seah S.Y., Britton K.L., Rice D.W., Asano Y., and Engel P.C. Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine. Biochemistry 41 (2002) 11390-11397