Molecular properties and enhancement of thermostability by random mutagenesis of glutamate dehydrogenase from Bacillus subtilis

Bioscience, Biotechnology and Biochemistry

Volumn 69, Issue 10, 2005, Pages 1861-1870

  Khan, Md Iqbal Hassan ^a   Ito, Kousuke ^a   Kim, Hyeung ^a   Ashida, Hiroyuki ^a   Ishikawa, Takahiro ^a   Shibata, Hitoshi ^a   Sawa, Yoshihiro ^a  

^a SHIMANE UNIVERSITY   (Japan)

Author keywords

Bacillus subtilis; Catalytic activity; Glutamate dehydrogenase; Random mutagenesis; Thermostability

Indexed keywords

AMINATION; BACTERIA; CATALYST ACTIVITY; CELLS; ENZYMES; ESCHERICHIA COLI; MOLECULAR STRUCTURE; MUTAGENESIS; REACTION KINETICS;

BACILLUS SUBTILIS; CATALYTIC ACTIVITY; GLUTAMATE DEHYDROGENASE; RANDOM MUTAGENESIS; THERMOSTABILITY;

THERMODYNAMIC STABILITY;

2 OXOGLUTARIC ACID; ALPHA-KETOGLUTARIC ACID; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; BACILLUS SUBTILIS; DIMERIZATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HEAT; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MISSENSE MUTATION; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTAGENESIS;

BACILLUS SUBTILIS; CLONING, MOLECULAR; DIMERIZATION; ENZYME STABILITY; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; HEAT; KETOGLUTARIC ACIDS; KINETICS; MOLECULAR WEIGHT; MUTAGENESIS; MUTATION, MISSENSE; NAD; SUBSTRATE SPECIFICITY;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 27644547321     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.69.1861     Document Type: Article

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