Recombinant Fab expression and secretion in Escherichia coli continuous culture at medium cell densities: Influence of temperature

Process Biochemistry

Volumn 47, Issue 3, 2012, Pages 446-452

  Rodríguez Carmona, Escarlata ^a,b   Cano Garrido, Olivia ^a,b   Dragosits, Martin ^c   Maurer, Michael ^c,d   Mader, Alexander ^c   Kunert, Renate ^c   Mattanovich, Diethard ^c   Villaverde, Antonio ^a,b   Vázquez, Felícitas ^a,b  

^a BIOMATERIALES Y NANOMEDICINA CIBER BBN   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^d Fachhochschule Campus Wien   (Austria)

Author keywords

Continuous culture; E. coli; Fab production; Process design; Process monitoring; Temperature influence

Indexed keywords

BIOMASS YIELD; CELL DENSITY; CELL PHYSIOLOGY; COMPREHENSIVE STUDIES; CONTINUOUS CULTIVATION; CONTINUOUS CULTURE; CONTROLLED CONDITIONS; CULTURE SUPERNATANT; E. COLI; ENVIRONMENTAL PARAMETER; EXPRESSION SYSTEM; FAB PRODUCTIONS; ITS EFFICIENCIES; PLASMID-BEARING CELLS; PROCESS DEVELOPMENT; PROCESS TEMPERATURE; PROCESS TIME; PRODUCT YIELDS; PROTEIN PRODUCTION; RECOMBINANT ANTIBODY; SELECTIVE PRESSURE; TEMPERATURE INFLUENCE; TEMPERATURE RANGE;

CELLS; CYTOLOGY; DNA; ESCHERICHIA COLI; PHYSIOLOGY; PROCESS DESIGN; PROCESS MONITORING;

CONTINUOUS CELL CULTURE;

ESCHERICHIA COLI;

EID: 84856211526     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2011.11.024     Document Type: Article

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