Rapid two-step purification of a recombinant mouse Fab fragment expressed in Escherichia coli

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 325-329

  Wlad, Hanna ^a   Ballagi, Andras ^a   Bouakaz, Lamine ^a   Gu, Zhenyu ^b   Janson, Jan Christer ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b INSTITUTE OF CHEMISTRY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; BETA LACTAM; COAT PROTEIN; CONCENTRATION (PARAMETERS); HOMOGENIZATION; IMMUNOGLOBULIN F(AB) FRAGMENT; PH; PROTEIN INDUCTION; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; SEPHAROSE; TOBACCO MOSAIC VIRUS;

ESCHERICHIA COLI; NICOTIANA TABACUM; TOBACCO MOSAIC VIRUS;

EID: 0034963168     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1444     Document Type: Article

References (19)

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6. Producing antibodies in Escherichia coli: From PCR to fermentation
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8. Comparative interaction kinetics of two recombinant Fab fragments and of the corresponding antibodies directed to the coat protein of tobacco mosaic virus
9. Theoretical analysis of protein concentration determination using biosensor technology under conditions of partial mass transport limitation
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11. Measurement of protein using bicinchoninic acid
12. High level production of soluble single chain antibodies in small-scale Escherichia coli cultures
13. Growth at sub-optimal temperatures allows the production of functional, antigen-binding Fab fragments in Escherichia coli
14. Bacterial expression and purification of the Fab fragment of a monoclonal antibody specific for the low-density lipoprotein receptor-binding site of human apolipoprotein E
15. Use of thiophilic adsorption chromatography for the one-step purification of a bacterially produced antibody Fab fragment without the need for an affinity tag
16. pSKAP/S: An expression vector for the production of single-chain fv alkaline phosphatase fusion proteins
17. Expression of a recombinant Fab antibody fragment against cruzipain, the major cysteine proteinase of Trypanosoma cruzi
18. Expression and purification of hexahistidine-tagged human glutathione S-transferase P1-1 in Escherichia coli
19. Preparation and uses of Fab' fragments from Escherichia coli