Co-expression of human protein disulphide isomerase (PDI) can increase the yield of an antibody Fab' fragment expressed in Escherichia coli

FEBS Letters

Volumn 380, Issue 1-2, 1996, Pages 194-197

  Humphreys, David P ^a   Weir, Neil ^b   Lawson, Alastair ^b   Mountain, Andrew ^b   Lund, Peter A ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b UCB PHARMA   (Belgium)

Author keywords

Antibody fragment; E. coli periplasm; Protein disulphide isomerase; Protein folding

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; PROTEIN DISULFIDE ISOMERASE;

ARTICLE; COLON CANCER; CONTROLLED STUDY; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; PRIORITY JOURNAL; TUMOR CELL;

ESCHERICHIA COLI; EUKARYOTA;

EID: 0030041230     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/0014-5793(96)00028-2     Document Type: Article

References (20)

1. Bardwell, J.C.A., McGovern, K. and Beckwith, J. (1991) Cell 67, 581-589.
2. Kamitani, S., Akiyama, Y. and Ito, K. (1992) EMBO J. 11, 57-62.
3. Bardwell, J.C.A., Lee, J.O., Jander, G., Martin, N., Belin, D. and Beckwith, J. (1993) Proc. Natl. Acad. Sci. USA 90, 1038-1042.
4. Missiakas, D., Georgopoulos, C. and Raina, S. (1993) Proc. Natl. Acad. Sci. USA 90, 7084-7088.
5. Missiakas, D., Georgopoulos, C. and Raina, S. (1994) EMBO J. 13, 2013-2020.
6. Missiakas, D., Schwager, F. and Raina, S. (1995) EMBO J. 14, 3415-3424.
7. Bardwell, J.C.A. (1994) Mol. Microbiol. 14, 199-205.
8. Freedman, R.B. (1989) Cell 57, 1069-1072.
9. Mazzarella, R.A., Srinivasan, M., Haugejorden, S.M. and Green, M. (1990) J. Biol. Chem. 265, 1094-1101.
10. Fullekrug, J., Sonnichsen, B., Wunsch, U., Arseven, K., Nguyen Van, P., Soling, H.D. and Mieskes, G. (1994) J. Cell Sci. 107, 2719-2727.
11. Srivastava, S.P., Fuchs, J.A. and Holtzman, J.L. (1993) Biochem. Biophys. Res. Commun. 193, 971-978.
12. Creighton, T.E. and Freedman, R.B. (1993) Curr. Biol. 3, 790-793.
13. Knappik, A., Krebber, C. and Pluckthun, A. (1993) Bio/Technology 11, 77-83.
14. Roth, R.A. and Pierce, S.B. (1987) Biochemistry 26, 4179-4182.
15. Lilie, H., McLaughlin, S., Freedman, R. and Buchner, J. (1994) J. Biol. Chem. 269, 14290-14296.
16. Humphreys, D.P., Weir, N., Mountain, A. and Lund, P.A. (1995) in press.
17. Emtage, J.S., Angal, S., Doel, M.T., Harris, T.J.R., Jenkins, B., Lilley, G. and Lowe, T.J.R. (1983) Proc. Natl. Acad. Sci. USA 80, 3671-3675.
18. Yarranton, G.T. and Mountain, A. (1992) in: Protein Engineering - a Practical Approach (Rees, A.R., Starnberg, M.J.E. and Wetzel, R. eds.) IRL Press, Oxford, pp. 303-326.
19. Leibovitz, A., Stinson, J.C., McCombs III, W.B., McCoy, C.E., Mazur, K.C. and Mabry, N.D. (1976) Cancer Res. 36, 4562-4569.
20. Cabilly, S. (1989) Gene 85, 553-557.