Optimization of extracellular production of recombinant asparaginase in Escherichia coli in shake-flask and bioreactor

Applied Microbiology and Biotechnology

Volumn 68, Issue 2, 2005, Pages 189-197

  Khushoo, Amardeep ^a   Pal, Yogender ^a   Mukherjee, K J ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BIOREACTORS; CELL CULTURE; CELLS; OPTIMIZATION; PROTEINS; PURIFICATION;

FEEDING STRATEGY; GROWTH RATE; PRODUCTION FORMATION;

ESCHERICHIA COLI;

ASPARAGINASE; BETA GALACTOSIDASE; HYBRID PROTEIN; ISOPROPYL BETA THIOGALACTOSIDE; RECOMBINANT ENZYME; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

BACTERIUM;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL DENSITY; CONTROLLED STUDY; DRUG ACTIVITY; DRUG PURIFICATION; DRUG SPECIFICITY; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; EXPRESSION VECTOR; EXTRACELLULAR MATRIX; FED BATCH REACTOR; GROWTH RATE; HOST CELL; NONHUMAN; NONVIRAL GENE THERAPY; OPTICAL DENSITY; PROMOTER REGION; TIME; VOLUMETRY;

ASPARAGINASE; BIOREACTORS; CULTURE MEDIA, CONDITIONED; ESCHERICHIA COLI; ISOPROPYL THIOGALACTOSIDE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 23444452242     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1867-0     Document Type: Article

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