Conversion of sterically demanding α,α-disubstituted phenylacetonitriles by the arylacetonitrilase from Pseudomonas fluorescens EBC191

Applied and Environmental Microbiology

Volumn 78, Issue 1, 2012, Pages 48-57

  Baum, Stefanie ^a   Williamson, Dael S ^b   Sewell, Trevor ^b   Stolz, Andreas ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOPHENONES; AMINO ACID RESIDUES; CHIRAL ANALYSIS; HOMOLOGY MODELS; MANIHOT ESCULENTA; NITRILASE; NITRILE GROUPS; OXYNITRILASES; PSEUDOMONAS FLUORESCENS; QUATERNARY CARBON; RECOMBINANT CELLS; RELATIVE ACTIVITIES; STERIC HINDRANCES; STERICALLY DEMANDING; SUBSTRATE SPECIFICITY;

AMIDES; AMINO ACIDS; BACTERIA; CATALYSTS; CHIRALITY; CYANIDES; ENANTIOMERS; ENZYMES; REACTION RATES; SUBSTRATES; SYNTHESIS (CHEMICAL);

KETONES;

2 PHENYLPROPIONITRILE; 2-PHENYLPROPIONITRILE; ACETONITRILE DERIVATIVE; ACETOPHENONE DERIVATIVE; AMMONIA; ARYLACETONITRILASE, PSEUDOMONAS FLUORESCENS EBC191; ATROLACTIC ACID; HYDROLASE; HYDROXYACID; LACTIC ACID DERIVATIVE; LYASE; MANDELONITRILE LYASE; NITRILASE; NITRILE;

AMINO ACID; BACTERIUM; CASSAVA; CATALYST; ENZYME ACTIVITY; HOMOLOGY; KETONE; MUTATION; STOICHIOMETRY; SUBSTRATE;

ARTICLE; BIOCATALYSIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MANIHOT; METABOLISM; PSEUDOMONAS FLUORESCENS; STEREOISOMERISM;

ACETONITRILES; ACETOPHENONES; ALDEHYDE-LYASES; AMINOHYDROLASES; AMMONIA; BIOCATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HYDROLYSIS; HYDROXY ACIDS; LACTATES; MANIHOT; NITRILES; PSEUDOMONAS FLUORESCENS; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

MANIHOT ESCULENTA; PSEUDOMONAS FLUORESCENS;

EID: 84855654946     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05570-11     Document Type: Article

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