Identification of amino acid residues responsible for the enantioselectivity and amide formation capacity of the arylacetonitrilase from Pseudomonas fluorescens EBC191

Applied and Environmental Microbiology

Volumn 75, Issue 17, 2009, Pages 5592-5599

  Kiziak, Christoph ^a,b   Stolz, Andreas ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b LONZA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE RESIDUES; ALCALIGENES FAECALIS; AMIDE FORMATION; AMINO ACID RESIDUES; AROMATIC RESIDUES; CHIMERIC ENZYMES; CYSTEINE RESIDUES; ENANTIOSPECIFICITY; MANDELIC ACID; NITRILASE; POINT MUTATIONS; PSEUDOMONAS FLUORESCENS; RELATIVE ACTIVITIES; TRYPTOPHAN RESIDUES;

AMIDES; AMINATION; AMINES; AROMATIC COMPOUNDS; ELECTRON TRANSITIONS; ENANTIOSELECTIVITY; ENZYMES; ORGANIC ACIDS;

AMINO ACIDS;

ACETONITRILE DERIVATIVE; ALANINE; AMIDE; AMINO ACID; CYSTEINE; ENZYME VARIANT; HISTIDINE; MANDELIC ACID; NITRILASE; PHENYLALANINE; TRYPTOPHAN; TYROSINE;

AMINO ACID; BACTERIUM; CATALYSIS; ENZYME ACTIVITY; HYDROLYSIS; MICROBIAL ACTIVITY; MUTATION;

ALCALIGENES FAECALIS; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME ACTIVITY; HYDROLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PROTEIN ANALYSIS; PSEUDOMONAS FLUORESCENS;

ACETONITRILES; ALCALIGENES FAECALIS; AMINO ACID SUBSTITUTION; AMINOHYDROLASES; CATALYTIC DOMAIN; MANDELIC ACIDS; MUTAGENESIS, SITE-DIRECTED; PSEUDOMONAS FLUORESCENS; RECOMBINANT FUSION PROTEINS; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

ALCALIGENES FAECALIS; BACTERIA (MICROORGANISMS); PSEUDOMONAS FLUORESCENS;

EID: 69449088689     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00301-09     Document Type: Article

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