Nitrilase from Pseudomonas fluorescens EBC191: Cloning and heterologous expression of the gene and biochemical characterization of the recombinant enzyme

Microbiology

Volumn 151, Issue 11, 2005, Pages 3639-3648

  Kiziak, Christoph ^a,b   Conradt, Doris ^a   Stolz, Andreas ^a   Mattes, Ralf ^a   Klein, Joachim ^a,b  

^a UNIVERSITY OF STUTTGART   (Germany)

^b LONZA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONITRILE DERIVATIVE; AMIDE; AMMONIUM SULFATE; BACTERIAL DNA; CARBOXYLIC ACID; MANDELIC ACID; NITRILASE; RECOMBINANT ENZYME;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; CHIRALITY; CONTROLLED STUDY; ENANTIOMER; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE ISOLATION; GENE SEQUENCE; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; TEMPERATURE;

AMINOHYDROLASES; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS FLUORESCENS;

EID: 27744507286     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.28246-0     Document Type: Article

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