Improved enantioselective conversion of styrene epoxides and meso-epoxides through epoxide hydrolases with a mutated nucleophile-flanking residue

Enzyme and Microbial Technology

Volumn 44, Issue 3, 2009, Pages 145-153

  van Loo, Bert ^a   Kingma, Jaap ^a   Heyman, Gertjan ^a   Wittenaar, Alex ^a   Lutje Spelberg, Jeffrey H ^a   Sonke, Theo ^b   Janssen, Dick B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b DSM Pharmaceutical Products   (Netherlands)

Author keywords

Biocatalysis; Enantioselectivity; Epoxide hydrolase; Mutagenesis; Screening

Indexed keywords

ACTIVE SITES; AGROBACTERIUM RADIOBACTER; AMINO ACID SUBSTITUTIONS; ASPARTATE; BINDING POCKETS; BIOCATALYSIS; BUTANEDIOL; CATALYTIC EFFICIENCIES; CATALYTIC PROPERTIES; CYCLOHEXENE OXIDES; ENANTIOSELECTIVE; EPOXIDE HYDROLASE; EPOXIDE HYDROLASES; GLYCIDYL ETHERS; HIGH YIELDS; PROGRESS CURVES; SINGLE PRODUCTS; STYRENE EPOXIDES; VICINAL DIOLS;

AMINES; AMINO ACIDS; CELL IMMOBILIZATION; CHIRALITY; ELECTRON TRANSITIONS; ENANTIOMERS; ENZYME ACTIVITY; ENZYMES; ETHERS; MUTAGENESIS; OLEFINS; ORGANIC ACIDS; PLANTS (BOTANY); STYRENE;

ENANTIOSELECTIVITY;

1,2 CYCLOHEXANEDIOL DERIVATIVE; CYCLOHEXANE OXIDE; ENZYME VARIANT; EPOXIDE HYDROLASE; NUCLEOPHILE; PHENYLALANINE; STYRENE OXIDE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ENANTIOSELECTIVITY; ENZYME STRUCTURE; GENE MUTATION; HYDROLYSIS; NONHUMAN; SPECTROPHOTOMETRY; WILD TYPE;

AGROBACTERIUM TUMEFACIENS;

EID: 58149477514     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2008.09.016     Document Type: Article

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