Influence of different carboxy-terminal mutations on the substrate-, reaction- and enantiospecificity of the arylacetonitrilase from Pseudomonas fluorescens EBC191

Protein Engineering, Design and Selection

Volumn 20, Issue 8, 2007, Pages 385-396

  Kiziak, Christoph ^a,b   Klein, Joachim ^a,b   Stolz, Andreas ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b LONZA AG   (Switzerland)

Author keywords

Enzyme catalysis; Mandelic acid; Nitrilase

Indexed keywords

ENZYME CATALYSIS; MANDELIC ACID; NITRILASE; PHENYLPROPIONITRILE;

AMINO ACIDS; CARBOXYLIC ACIDS; ENANTIOMERS; ENANTIOSELECTIVITY; REACTION KINETICS;

ENZYME KINETICS;

2 PHENYLPROPIONITRILE; AMIDE; AMINO ACID; BACTERIAL ENZYME; CHIMERIC PROTEIN; ENZYME VARIANT; HISTIDINE; MANDELONITRILE; NITRILASE; NITRILE; UNCLASSIFIED DRUG;

ALCALIGENES FAECALIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPARATIVE STUDY; CONTROLLED STUDY; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME SUBSTRATE; ENZYME SYNTHESIS; GENE DELETION; GENE MUTATION; MUTAGENESIS; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; REACTION ANALYSIS; RHODOCOCCUS; WILD TYPE;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINOHYDROLASES; BASE SEQUENCE; BINDING SITES; CATALYSIS; DIMERIZATION; DNA, BACTERIAL; ENZYME STABILITY; ESCHERICHIA COLI; FREEZING; HYDROGEN BONDING; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PSEUDOMONAS FLUORESCENS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

ALCALIGENES FAECALIS; BACTERIA (MICROORGANISMS); PSEUDOMONAS FLUORESCENS; RHODOCOCCUS RHODOCHROUS;

EID: 34548773501     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm032     Document Type: Article

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