An amino acid at position 142 in nitrilase from Rhodococcus rhodochrous ATCC 33 278 determines the substrate specificity for aliphatic and aromatic nitriles

Biochemical Journal

Volumn 415, Issue 3, 2008, Pages 401-407

  Yeom, Soo Jin ^a   Kim, Hye Jung ^a   Lee, Jung Kul ^a,b   Kim, Dong Eun ^a   Oh, Deok Kun ^a  

^a Konkuk University   (South Korea)

^b Konkuk University   (South Korea)

Author keywords

Aliphatic nitrile; Aromatic nitrile; Nitrilase; Rhodococcus rhodochrous; Substrate specificity

Indexed keywords

ALIPHATIC NITRILE; AROMATIC NITRILE; NITRILASE; RHODOCOCCUS RHODOCHROUS; SUBSTRATE SPECIFICITY;

AMINATION; AMINES; AMINO ACIDS; AROMATIC COMPOUNDS; AROMATIZATION; CYANIDES; ENZYME ACTIVITY; ENZYMES; HYDROLYSIS; HYDROPHOBICITY; ORGANIC ACIDS;

SUBSTRATES;

2 CYANOPYRIDINE; 3 TOLUNITRILE; ADIPONITRILE; ALANINE; ARGININE; AROMATIC AMINO ACID; AROMATIC COMPOUND; AROMATIC NITRILE; ASPARAGINE; ASPARTIC ACID; BENZONITRILE; GLUTARONITRILE; LEUCINE; MUTANT PROTEIN; NITRILASE; NITRILE; SEBACONITRILE; SUCCINONITRILE; TYROSINE; UNCLASSIFIED DRUG; VALINE; AMINO ACID; GLUTAMIC ACID; HYDROLASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENE; CHEMICAL STRUCTURE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HYDROLYSIS; MOLECULAR MODEL; MOLECULAR WEIGHT; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RHODOCOCCUS; RHODOCOCCUS RHODOCHROUS ATCC 33 278; SEQUENCE ALIGNMENT; CATALYSIS; CHEMISTRY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; SITE DIRECTED MUTAGENESIS;

RHODOCOCCUS RHODOCHROUS;

AMINO ACID SEQUENCE; AMINO ACIDS; AMINOHYDROLASES; ARGININE; ASPARAGINE; ASPARTIC ACID; CATALYSIS; GLUTAMIC ACID; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; NITRILES; RHODOCOCCUS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

EID: 58149142732     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20080440     Document Type: Article

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