Investigation of the structure and proteolytic activity of papain in aqueous miscible organic media

Process Biochemistry

Volumn 47, Issue 1, 2012, Pages 47-56

  Llerena Suster, Carlos R ^a   José, Carla ^b   Collins, Sebastián E ^c   Briand, Laura E ^b   Morcelle, Susana R ^a  

^a UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^b DEPARTAMENTO DE QUÍMICA   (Argentina)

^c UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

Author keywords

Aqueous organic media; ATR FTIR spectroscopy; Catalytic stability; Fluorescence spectroscopy; Papain; Structural stability; Thermal stability

Indexed keywords

AQUEOUS-ORGANIC MEDIA; ATR-FT-IR SPECTROSCOPY; CATALYTIC STABILITY; PAPAIN; STRUCTURAL STABILITIES;

ACETONITRILE; AMIDES; ENZYME ACTIVITY; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; FOURIER TRANSFORM INFRARED SPECTROSCOPY; METHANOL; ORGANIC SOLVENTS; SPECTROSCOPIC ANALYSIS; THERMODYNAMIC STABILITY;

CATALYST ACTIVITY;

EID: 83655184741     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2011.10.003     Document Type: Article

References (55)

1. D. Choudhury, S. Roy, C. Chakrabarti, S. Biswas, and J.K. Dattagupta Production and recovery of recombinant propapain with high yield Phytochemistry 70 2009 465 472 and references therein
2. M.K. Ramjee, J.R. Petithory, J. McElver, S.C. Weber, and J.F. Kirsch A novel yeast expression/secretion system for the recombinant plant thiol endoprotease propapain Prot Eng 9 1996 1055 1061 and references therein (Pubitemid 26402309)
3. N.D. Rawlings, and Barrett AJ Introduction: the clans and families of cysteine peptidases A. Barrett, N.D. Rawlings, J.F. Woessner, Handbook of proteolytic enzymes 2004 Academic Press London 1051 1071
4. K. Sangeetha, and T.E. Abraham Chemical modification of papain for use in alkaline medium J Mol Catal B: Enzym 38 2006 171 177
5. J. Chamani, and M. Heshmati Mechanism for stabilization of the molten globule state of papain by sodium n-alkyl sulfates: spectroscopic and calorimetric approaches Colloid Interface Sci 322 2008 119 127
6. H.A. Sathish, P.R. Kumar, and V. Prakash Mechanism of solvent induced thermal stabilization of papain Int J Biol Macromol 41 2007 383 390 (Pubitemid 47331626)
7. H.A. Sathish, P.R. Kumar, and V. Prakash The differential stability of the left and right domains of papain Process Biochem 44 2009 710 716
8. F. Edwin, and M.V. Jagannadham Sequential unfolding of papain in molten globule state Biochem Biophys Res Commun 252 1998 654 660 (Pubitemid 28556832)
9. J.A. Feliu, C. de Mas, and J. Lopez-Santin Studies on papain action in the synthesis of Gly-Phe in 2-liquid-phase media Enzyme Microb Technol 17 1995 882 887
10. T. Theppakorn, P. Kanasawud, and P.J. Halling Effect of solid-state buffers on the catalytic activity of papain in low water media Enzyme Microb Technol 32 2003 828 836 (Pubitemid 36589025)
11. S. Ghosh Conformational study of papain in the presence of sodium dodecyl sulfate in aqueous medium Colloid Surf B: Biointerfaces 41 2005 209 216 (Pubitemid 40298836)
12. L. Panella, J. Broos, J. Jin, M.W. Fraaije, D.B. Janssen, M. Jeronimus-Stratingh, B.L. Feringa, A.J. Minnaard, and J.G. de Vries Merging homogeneous catalysis with biocatalysis; papain as hydrogenation catalyst Chem Commun 45 2005 5656 5658 (Pubitemid 41785188)
13. A. Szabó, M. Kotórman, I. Laczkó, and L.M. Simon Spectroscopic studies of stability of papain in aqueous organic solvents J Mol Catal B: Enzym 41 2006 43 48 (Pubitemid 43903351)
14. W.-Y. Lou, M.-H. Zong, T.J. Smith, H. Wu, and J.-F. Wang Impact of ionic liquids on papain: an investigation of structure-function relationships Green Chem 8 2006 509 512 (Pubitemid 43856601)
15. A. Lang, C. Hatscher, and P. Kuhl Papain-catalysed synthesis of Z-l-aminoacyl-antipyrine amides from Z-protected amino acid esters and 4-aminoantipyrine Tetrahedron Lett 48 2007 3371 3374 (Pubitemid 46574797)
16. A. Szabó, M. Kotormán, I. Laczkó, and L.M. Simon Improved stability and catalytic activity of chemically modified papain in aqueous organic solvents Process Biochem 44 2009 199 204
17. Y. Zhang, G. Shi, and F. Zhao Hydrolysis of casein catalyzed by papain in n-propanol/NaCl two-phase system Enzyme Microb Technol 46 2010 438 449
18. M. Wang, C. Jia, W. Qi, Q. Yu, X. Peng, R. Su, and Z. He Porous-CLEAs of papain: application to enzymatic hydrolysis of macromolecules Bioresour Technol 102 2011 3541 3545
19. M. Jackson, and H.H. Mantsch The use and misuse of FTIR spectroscopy in the determination of protein structure Crit Rev Biochem Mol Biol 30 1995 95 120
20. E. Goormaghtigh, V. Cabiaux, and J.M. Ruysschaert Secondary structure and dosage of soluble and membrane proteins by ATR-FTIR on hydrated films Eur J Biochem 193 1990 409 420
21. S.R. Morcelle, S. Barberis, N. Priolo, N.O. Caffini, and P. Clapés Comparative behaviour of proteinases from the latex of Carica papaya and Funastrum clausum as catalysts for the synthesis of Z-Ala-Phe-OMe J Mol Catal B: Enzym 41 2006 117 124 and references therein (Pubitemid 44160561)
22. C.R. Llerena-Suster, W.D. Obregón, S.A. Trejo, and S.R. Morcelle Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses Anal Lett 44 2011 2124 2137
23. C.R. Llerena-Suster, N.S. Priolo, and S.R. Morcelle Sodium tetrathionate effect on papain purification from different crude extracts obtained from Carica papaya latex Prep Biochem Biotechnol 41 2011 107 121
24. N. Priolo, L.M.I. López, M.C. Arribére, C.L. Natalucci, and N.O. Caffini New purified plant proteinases for the food industry Acta Aliment 20 1991 189 196
25. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1976 248 254
26. I.G. Kamphuis, K.H. Kalk, M.B.A. Swarte, and J. Drenth Structure of papain refined at 1.65 resolution J Mol Biol 179 1984 233 256
27. I. Schechter, and A. Berger On the size of the active site of proteases. I. Papain Biochem Biophys Res Commun 27 1967 157 162
28. R. Ménard, and A. Storer Papain A. Barrett, N.D. Rawlings, J.F. Woessner, Handbook of proteolytic enzymes 2004 Academic Press London 1126
29. M.M. Fernandez, A.O. Margot, C.A. Falender, H.W. Blanch, and D.S. Clark Enzymatic synthesis of peptides containing unnatural amino acids Enzyme Microb Technol 17 1995 964 971
30. H.-Y. Shen, G.-L. Tian, Y.-H. Ye, and J. Wang Non-coded amino acids as acyl donor substrates for peptide bond formation catalyzed by thermoase in toluene J Mol Catal B: Enzym 37 2005 26 29 (Pubitemid 41674644)
31. D. Cantacuzne, F. Pascal, and C. Guerreiro Synthesis of amino acid esters by papain Tetrahedron 43 1987 1823 1826
32. M.M. Fernández, D.S. Clark, and H.W. Blanch Papain kinetics in the presence of a water-miscible organic solvent Biotechnol Bioeng 37 1991 967 972
33. M. Fité, G. Alvaro, P. Clapés, J. López- Santín, M.D. Benaiges, and G. Caminal Reactivity of easily removable protecting groups for glycine in peptide synthesis using papain as catalyst Enzyme Microb Technol 23 1998 199 203 (Pubitemid 28422871)
34. P. Stehle, H.-P. Bahsitta, B. Monter, and P. Fürst Papain-catalysed synthesis of dipeptides: A novel approach using free amino acids as nucleophiles Enzyme Microb Technol 12 1990 56 60 (Pubitemid 20082431)
35. D.E. Stevenson, and A.C. Storer Papain in organic solvents: determination of conditions suitable for biocatalysis and the effect on substrate specificity and inhibition Biotechnol Bioeng 37 1991 519 527
36. P. Clapés, J.L. Torres, and P. Adlercreutz Enzymatic peptide synthesis in low water content systems: preparative enzymatic synthesis of [Leu]- and [Met]-enkephalin derivatives Bioorg Med Chem 3 1995 245 255
37. S.R. Morcelle, C.S. Liggieri, M.A. Bruno, N. Priolo, and P. Clapés Screening of plant peptidases for the synthesis of arginine-based surfactants J Mol Catal B: Enzym 57 2009 177 182
38. 2 Biocatal Biotransform 17 2000 417 429 (Pubitemid 30110354)
39. D.-F. Tai, S.-L. Fu, S.-F. Chuang, and H. Tsai Papain catalyzed esterification in polar organic solvents Biotechnol Lett 11 1989 173 176
40. L.G. Gebhard, F.U. Carrizo, A.L. Stern, N.I. Burgardt, J. Faivovich, E. Lavilla, and M.R. Ermácora A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii Eur J Biochem 271 2004 2117 2126 (Pubitemid 38747981)
41. J.V. Potetinova, T.L. Voyushina, and V.M. Stepanov Enzymatic síntesis of peptidyl amino alcohols and peptidyl amino aldehydes-serine proteinase inhibitors Bioorg Med Chem 7 1999 705 710 (Pubitemid 27121183)
42. A.V. Bacheva, I.Y. Filippova, E.N. Lysogorskaya, and E.S. Oksenoit Stability and catalytic properties of subtilisin in acetonitrile/ dimethylformamide mixtures with low water content J Mol Catal B: Enzym 11 2000 89 96
43. K. Kijima, S. Yamamoto, and H. Kise Study on tryptophan fluorescence and catalytic activity of α-chymotrypsin in aqueous-organic media Enzyme Microb Technol 18 1996 2 6 (Pubitemid 26059404)
44. V. Herlax, and L. Bakás Fatty acids covalently bound to R-hemolysin of Escherichia coli are involved in the molten globule conformation: implication of disordered regions in binding promiscuity Biochemistry 46 2007 5177 5184 (Pubitemid 46683016)
45. A. Barth Infrared spectroscopy of proteins Biochim Biophys Acta 1767 2007 1073 1101 (Pubitemid 47313388)
46. A. Dong, J. Matsuura, J.D. Allison, E. Chrisman, M.C. Manning, and J.F. Carpenter Infrared and circular dicroism spectroscopic characterization of structural differences between β-lactoglobulin A and B Biochemistry 35 1996 1450 1457 (Pubitemid 26055280)
47. J. Kong, and S. Yu Fourier transform infrared spectroscopic analysis of protein secondary structures Acta Biochim Biophys Sin 39 2007 549 559 (Pubitemid 47293710)
48. A. Barth, and C. Zscherp What vibrations tell us about proteins Q Rev Biophys 35 2002 369 430 (Pubitemid 36207229)
49. A. Natalello, D. Ami, S. Brocca, M. Lotti, and Doglia SM Secondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopy Biochem J 385 2005 511 517 (Pubitemid 40165084)
50. P.I. Haris, and F. Severcan FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media J Mol Catal B: Enzym 7 1999 207 221 (Pubitemid 29408531)
51. M.L. Foresti, G. Valle, R. Bonetto, M.L. Ferreira, and Briand LE FTIR, SEM and fractal dimension characterization of lipase B from Candida antarctica immobilized onto titania at selected conditions Appl Surf Sci 256 2010 1624 1635
52. R.M. Lau, M.J. Sorgedrager, G. Carrea, F. van Rantwijk, F. Secundo, and R.A. Sheldon Dissolution of Candida antarctica lipase B in ionic liquids: effects on structure and activity Green Chem 6 2004 483 487 (Pubitemid 39472347)
53. K.A. Oberg, J.M. Ruysschaert, and E. Goormarghtigh Rationally selected bases proteins: a new approach to selecting proteins for spectroscopic secondary structure analysis Protein Sci 12 2003 2015 2031 (Pubitemid 37022823)
54. 13C-Labeled tyrosine residues as local IR probes for monitoring conformational changes in peptides and proteins Angew Chem Int Ed 44 2005 4631 4635 (Pubitemid 41026410)
55. F. Bordusa Proteases in organic synthesis Chem Rev 102 2002 4817 4867