Porous-CLEAs of papain: Application to enzymatic hydrolysis of macromolecules

Bioresource Technology

Volumn 102, Issue 3, 2011, Pages 3541-3545

  Wang, Mengfan ^a   Jia, Chenxi ^a   Qi, Wei ^a   Yu, Qingxin ^a   Peng, Xin ^a   Su, Rongxin ^a   He, Zhimin ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

Enzymatic hydrolysis; Enzyme immobilization; Macromolecule; Papain; Porous cross linked enzyme aggregates

Indexed keywords

BOVINE SERUM ALBUMINS; CATALYTIC EFFICIENCIES; CROSS-LINKED ENZYME AGGREGATES; NOVEL STRATEGIES; OVALBUMINS; PAPAIN; POROUS STRUCTURES; SCANNING ELECTRON MICROSCOPES;

BODY FLUIDS; ENZYME IMMOBILIZATION; ENZYMES; MACROMOLECULES; SCANNING ELECTRON MICROSCOPY; STARCH;

ENZYMATIC HYDROLYSIS;

AMYLASE; BOVINE SERUM ALBUMIN; OVALBUMIN; PAPAIN; STARCH;

CATALYSIS; ENZYME ACTIVITY; HYDROLYSIS; IMMOBILIZATION; MOLECULAR ANALYSIS; POLLUTANT REMOVAL; SCANNING ELECTRON MICROSCOPY; SERUM; STARCH;

ARTICLE; CATALYSIS; CHEMICAL STRUCTURE; CROSS LINKED ENZYME AGGREGATE; CROSS LINKING; HYDROLYSIS; IMMOBILIZATION; POROSITY; PRECIPITATION; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY;

ENZYME ACTIVATION; ENZYMES, IMMOBILIZED; HYDROLYSIS; MACROMOLECULAR SUBSTANCES; PAPAIN; POROSITY; STARCH;

BOVINAE;

EID: 78650817240     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.08.120     Document Type: Article

References (19)

1. Aytar B.S., Bakir U. Preparation of cross-linked tyrosinase aggregates. Process Biochemistry 2008, 43:125-131.
2. Cabirol F.L., Hanefeld U., Sheldon R.A. Immobilized hydroxynitrile lyases for enantioselective synthesis of cyanohydrins: sol-gels and cross-linked enzyme aggregates. Advanced Synthesis and Catalysis 2006, 348:1645-1654.
3. Cao L.Q., van Rantwijk F., Sheldon R.A. Cross-linked enzyme aggregates: a simple and effective method for the immobilization of penicillin acylase. Organic Letters 2000, 2:1361-1364.
4. Cao L., van Langen L.M., van Rantwijk F., Sheldon R.A. Cross-linked aggregates of penicillin acylase: robust catalysts for the synthesis of β-lactam antibiotics. Journal of Molecular Catalysis B: Enzymatic 2001, 11:665-670.
5. 2-expanded micellar solutions. Colloids and Surfaces B: Biointerfaces 2006, 48:72-76.
6. Dalal S., Kapoor M., Gupta M.N. Preparation and characterization of combi-CLEAs catalyzing multiple non-cascade reactions. Journal of Molecular Catalysis B: Enzymatic 2007, 44:128-132.
7. Kazuo O., Hidemi K., Takashi A. Biosynthesis and excretion of hydrolases in germinating cereal seeds. Plant and Cell Physiology 1980, 21:201-204.
8. Lopez-Serrano P., Cao L., van Rantwijk F., Sheldon R.A. Cross-linked enzyme aggregates with enhanced activity: application to lipases. Biotechnology Letters 2002, 24:1379-1383.
9. Matijošyte I., Arends I.W.C.E., De Vries S., Sheldon R.A. Preparation and use of cross-linked enzyme aggregates (CLEAs) of laccases. Journal of Molecular Catalysis B: Enzymatic 2010, 62:142-148.
10. Montoro-García S., Gil-Ortiz F., Navarro-Fernández J., Rubio V., García-Carmona F., Sánchez-Ferrer Á. Improved cross-linked enzyme aggregates for the production of desacetyl β-lactam antibiotics intermediates. Bioresource Technology 2010, 101:331-336.
11. Olson, B.J.S.C., Markwell, J., 2007. Assays for determination of protein concentration. Current Protocols in Protein Science. John Wiley, New York.
12. Prabhavathi Devi B.L.A., Guo Z., Xu X. Characterization of cross-linked lipase aggregates. Journal of the American Oil Chemists' Society 2009, 86:637-642.
13. Schoevaart R., Wolbers M.W., Golubovic M., Ottens M., Kieboom A.P.G., van Rantwijk F., van der Wielen L.A.M., Sheldon R.A. Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs). Biotechnology and Bioengineering 2004, 87:754-762.
14. Sheldon R.A., Schoevaart R., Vanlangen L.M. Cross-linked enzyme aggregates (CLEAs): a novel and versatile method for enzyme immobilization. Biocatalysis and Biotransformation 2005, 23:141-147.
15. Stellmach, B., Gottschick, W., 1988. Bestimmungsmethoden Enzyme, Steinkopff Verlag, Darmstadt.
16. Vafiadi C., Topakas E., Christakopoulos P. Preparation of multipurpose cross-linked enzyme aggregates and their application to production of alkyl ferulates. Journal of Molecular Catalysis B: Enzymatic 2008, 54:35-41.
17. van Pelt S., Quignard S., Kubac D., Dimitry Y.S.B., van Rantwijk F., Sheldon R.A. Nitrile hydratase CLEAs: the immobilization and stabilization of an industrially important enzyme. Green Chemistry 2008, 10:395-400.
18. Wang M. Carrier-immobilized Cross-linked Enzyme Aggregates and Its Application in Alanyl-glutamine Dipeptide Synthesis 2006, Tianjin University, Tianjin.
19. Youngkyu C., Chulhee K. Synthesis and photophysical characterization of amphiphilic dendritic-linear-dendritic block copolymers. Journal of Polymer Science Part A: Polymer Chemistry 2001, 39:918-926.