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Volumn , Issue 45, 2005, Pages 5656-5658

Merging homogeneous catalysis with biocatalysis; papain as hydrogenation catalyst

Author keywords

[No Author keywords available]

Indexed keywords

ACRYLIC ACID DERIVATIVE; METHYL 2 ACETAMIDOACRYLATE; PAPAIN; UNCLASSIFIED DRUG;

EID: 28944451442     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/b512138h     Document Type: Article
Times cited : (83)

References (57)
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    • For recent overviews see: (a) F. H. Arnold, Nature, 2001, 409, 253;
    • (2001) Nature , vol.409 , pp. 253
    • Arnold, F.H.1
  • 14
    • 28944448011 scopus 로고    scopus 로고
    • (e) N. E. Labrou, Biomol. Eng., 2005, 22, 1-3, thematic issue on directed enzyme evolution.
    • (2005) Biomol. Eng. , vol.22 , pp. 1-3
    • Labrou, N.E.1
  • 15
    • 0035910597 scopus 로고    scopus 로고
    • (a) For a review on high-throughput ee-assays see: M. T. Reetz, Angew. Chem., Int. Ed., 2001, 40, 284;
    • (2001) Angew. Chem., Int. Ed. , vol.40 , pp. 284
    • Reetz, M.T.1
  • 17
    • 0035449011 scopus 로고    scopus 로고
    • (c) For a review on enantioselective miniaturized detectors see: T. J. Edkins and D. R. Bobbitt, Anal. Chem., 2001, 73, 488A.
    • (2001) Anal. Chem. , vol.73
    • Edkins, T.J.1    Bobbitt, D.R.2
  • 36
    • 28944436044 scopus 로고    scopus 로고
    • note
    • We hoped that additional binding sites for Rh(I), if necessary, could be found between the residues in the enzyme pocket, as for example His-159 positioned opposite Cys-25.
  • 48
    • 0035971296 scopus 로고    scopus 로고
    • For a review on decomposition pathways in catalysis see: P. W. N. M. van Leeuwen, Appl. Catal., A, 2001, 212, 61.
    • (2001) Appl. Catal., A , vol.212 , pp. 61
    • Van Leeuwen, P.W.N.M.1
  • 49
    • 28944446554 scopus 로고    scopus 로고
    • note
    • 2O/acetone (δ 137.6).
  • 51
    • 28944454138 scopus 로고    scopus 로고
    • note
    • In the spectrum a peak of residual oxidized papain (as its sulfinic acid) is also visible, together with a peak corresponding to the solely attached 3-hydroxy-phenacyl group resulting from the hydrolysis of the phosphite ligand. The presence of this peak seemed to be partially caused by the presence of formic acid necessary for the ESI-MS analysis.
  • 52
    • 28944447095 scopus 로고    scopus 로고
    • note
    • 4 were used in the complexation step.
  • 54
    • 28944447622 scopus 로고    scopus 로고
    • note
    • An additional peak appears in the ESI-MS, that could be attributed to an adduct between papain in its reduced state and one unit of Rh(I)(COD).
  • 55
    • 28944449434 scopus 로고    scopus 로고
    • note
    • 2 pressure was also obtained when the substrate to catalyst ratio was increased to 800 : 1.
  • 56
    • 28944433302 scopus 로고    scopus 로고
    • note
    • The active site of papain is placed in a hydrophobic pocket with a cleft of about 15 Å and a groove of about 25 Å.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.